Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 990033
View all Troponin T Primary Antibodies »

Product Specifications

Immunogen

Human Troponin T (Cardiac) antigen
Accession # P45379

Specificity

Detects human Troponin T in ELISA. Detects human, mouse, and rat Troponin T in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat Troponin T (Cardiac) Antibody

Detection of Human, Mouse, and Rat Troponin T antibody by Western Blot.

Detection of Human, Mouse, and Rat Troponin T by Western Blot.

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Troponin T at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Troponin T antibody in Human Heart by Immunohistochemistry (IHC-P).

Troponin T in Human Heart.

Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Human Troponin T (Cardiac) Antibody in ELISA Standard Curve.

Human Troponin T (Cardiac) ELISA Standard Curve.

Recombinant Human Troponin T protein was serially diluted 2-fold and captured by Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human/Mouse/Rat Troponin T (Cardiac) Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody to detect human Troponin T (Cardiac) when paired with Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18741).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Western Blot

0.1 µg/mL
Sample: Human heart tissue, Mouse heart tissue, and Rat heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Troponin T

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Alternate Names

CMH2, CMPD2, cTnT, TNNT2, TnTC

Entrez Gene IDs

7139 (Human)

Gene Symbol

TNNT2

UniProt

P45379

Additional Troponin T Products

Product Documents for Human/Mouse/Rat Troponin T (Cardiac) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Troponin T (Cardiac) Antibody

For research use only

Related Research Areas

Cardiac Stem Cell Markers

Citations for Human/Mouse/Rat Troponin T (Cardiac) Antibody

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Protocols

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FAQs

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