Rat IL-10 Antibody
Rat IL-10 Antibody Summary
Ser19-Asn178
Accession # P29456
Applications
Rat IL-10 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Mouse and Rat IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Rat IL-10 (Catalog # 522-RLB), Recombinant Human IL-10 (Catalog # 217-IL) and Recombinant Mouse IL-10 (Catalog # 417-ML). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Rat IL-10 Monoclonal Antibody (Catalog # MAB519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10
Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
Product Datasheets
Citations for Rat IL-10 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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The role of cyclosporine and mycophenolate in an orthotopic porcine-to-rat corneal xenotransplantation.
Authors: Lee HI, Kim MK, Oh JY, Ko JH, Lee HJ, Wee WR, Lee JH
J. Korean Med. Sci., 2008;23(3):492-501.
Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development -
The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
Stem Cells, 2008;26(4):1047-55.
Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development -
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Authors: Roche M, Diamond M, Finn DP
J. Neuroimmunol., 2006;181(1):57-67.
Species: Rat
Sample Types: Plasma
Applications: ELISA Development
FAQs
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Reconstitution Buffers
Secondary Antibodies
Reviews for Rat IL-10 Antibody
Average Rating: 5 (Based on 1 Review)
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This antibody was used to build an ELISA to measure rat IL-10. This antibody worked well as a capture antibody and BAF519 as the detection antibody.
