Rat IL-1 beta /IL-1F2 Antibody

(27 citations)
(1 Review)
  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL‑1 beta is observed and less than 0.5% cross-reactivity with recombinant human IL-1 beta and recombinant porcine IL-1 beta is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant rat IL-1 beta /IL-1F2 (R&D Systems, Catalog # 501-RL)
    Val117-Ser268
    Accession # Q63264
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Rat IL-1 beta /IL-1F2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Rat IL‑1 beta /IL‑1F2 Antibody (Catalog # AF-501-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Rat IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF501)
  • ELISA Standard
     
    Recombinant Rat IL-1 beta/IL-1F2 Protein (Catalog # 501-RL)
  • Neutralization
    Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑1 beta /IL‑1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND50 is typically 2-10 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Immunocytochemistry
IL‑1 beta /IL‑1F2 in Rat Splenocytes. IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65-77% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.

  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Microglia-derived IL-1? promotes chemokine expression by M�ller cells and RPE in focal retinal degeneration
    Authors: R Natoli, N Fernando, M Madigan, JA Chu-Tan, K Valter, J Provis, M Rutar
    Mol Neurodegener, 2017;12(1):31.
    Species: Rat
    Sample Type: In Vivo
    Application: Neut
  2. Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia
    Authors: V Hernandez-, A Cabrera-Pa, L Taoro-Gonz, A Gonzalez-U, A Agusti, T Balzano, M Llansola, V Felipo
    J Neuroinflammation, 2016;13(1):83.
  3. In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues
    Molecules, 2016;21(8):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  4. Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration.
    Authors: Jiao H, Natoli R, Valter K, Provis J, Rutar M
    PLoS ONE, 2015;10(12):e0143952.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. The spinal anti-inflammatory mechanism of motor cortex stimulation: cause of success and refractoriness in neuropathic pain?
    Authors: Silva G, Lopes P, Fonoff E, Pagano R
    J Neuroinflammation, 2015;12(0):10.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  6. Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure.
    Authors: Madeira M, Elvas F, Boia R, Goncalves F, Cunha R, Ambrosio A, Santiago A
    J Neuroinflammation, 2015;12(0):115.
    Species: Rat
    Sample Type: Tissue Culture Supernates
    Application: Neut
  7. Spinal cord injury induces a long-lasting upregulation of interleukin-1beta in astrocytes around the central canal.
    Authors: Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, Molina-Holgado F, Garcia-Ovejero D
    Neuroscience, 2015;284(0):283-9.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  8. Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
    Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K
    J Neurosci, 2014;34(6):2231-43.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  9. Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
    Authors: Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang T
    PLoS ONE, 2014;9(8):e106331.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration.
    Authors: Liu R, Gao J, Cao S, Sandhu N, Cui J, Chou C, Fang E, Matsubara J
    Invest Ophthalmol Vis Sci, 2013;54(3):2225-37.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
    J Neuroinflammation, 2012;9(0):181.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  12. Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes.
    Authors: Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F
    J. Neurochem., 2011;117(1):164-74.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  13. Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke.
    Authors: Faustino JV, Wang X, Johnson CE
    J. Neurosci., 2011;31(36):12992-3001.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  14. Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
    Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
    J. Neurochem., 2011;116(2):217-26.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  15. Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
    Authors: Summy-Long JY, Hu S
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(5):R1532-45.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  16. Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
    Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J
    J. Clin. Invest., 2009;119(2):295-304.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  17. Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland.
    Authors: Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A
    Endocrinology, 2008;149(4):1436-50.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  18. Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
    Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
    Infect. Immun., 2008;76(8):3577-86.
    Species: Rat
    Sample Type: BALF
    Application: ELISA Development
  19. Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
    Authors: Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(3):H1839-46.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  20. IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.
    Authors: Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H
    Biochem. Biophys. Res. Commun., 2007;365(2):375-80.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  21. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
    Authors: Barkho BZ, Song H, Aimone JB
    Stem Cells Dev., 2006;15(3):407-21.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  22. In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
    Authors: Roche M, Diamond M, Finn DP
    J. Neuroimmunol., 2006;181(1):57-67.
    Species: Rat
    Sample Type: Plasma
    Application: ELISA Development
  23. Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines.
    Authors: Assmus M, Svechnikov K, von Euler M, Setchell B, Sultana T, Zetterstrom C, Holst M, Soder O
    Pediatr. Res., 2005;57(6):896-901.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  24. Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
    Authors: Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S
    Neuroscience, 2005;132(3):625-31.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  25. A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity.
    Authors: Guo L, LaDu MJ, Van Eldik LJ
    J. Mol. Neurosci., 2004;23(3):205-12.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  26. Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
    Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H
    J. Physiol. (Lond.), 2004;556(0):193-202.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  27. Glucocorticoids play a fundamental role in protecting the brain during innate immune response.
    Authors: Nadeau S, Rivest S
    J. Neurosci., 2003;23(13):5536-44.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
Expand to show all 27 Citations
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Average Rating: 5 (Based on 1 review)

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ELISA Rat IL-1 beta /IL-1F2 Antibody AF-501-NA
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ELISA Rat IL-1 beta /IL-1F2 Antibody AF-501-NA
ELISA: Rat IL-1 beta /IL-1F2 Antibody [AF-501-NA]

Summary

ApplicationELISA
Sample TestedSerum

Other Experimental Details

Other Experimental DetailsThis polyclonal antibody was used as both a capture and detection (the biotinylated version of this antibody, BAF501, was used as the detection) in a sandwich ELISA. Rat IL-1b was successful measured in serum samples.

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