Rat IL-1 beta /IL-1F2 Antibody

Catalog # Availability Size / Price Qty
AF-501-NA
AF-501-SP
Rat IL-1 beta /IL-1F2 Antibody in Data
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Product Details
Citations (38)
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Rat IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL‑1 beta is observed and less than 0.5% cross-reactivity with recombinant human IL-1 beta and recombinant porcine IL-1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat IL-1 beta /IL-1F2 (R&D Systems, Catalog # 501-RL)
Val117-Ser268
Accession # Q63264
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL)
Immunocytochemistry
5-15 µg/mL
See below

Rat IL-1 beta /IL-1F2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Rat IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF501)

Standard: Recombinant Rat IL-1 beta/IL-1F2 Protein (Catalog # 501-RL)

Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND50is typically 2-10 µg/mL.

Immunocytochemistry IL‑1 beta /IL‑1F2 in Rat Splenocytes. View Larger

IL‑1 beta /IL‑1F2 in Rat Splenocytes. IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

Citations for Rat IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Of Mice and Monkeys: Neuroprotective Efficacy of the p38 Inhibitor BIRB 796 Depends on Model Duration in Experimental Glaucoma
    Authors: WS Lambert, S Pasini, JW Collyer, CR Formichell, P Ghose, BJ Carlson, DJ Calkins
    Sci Rep, 2020;10(1):8535.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Microglial activation by microbial neuraminidase through TLR2 and TLR4 receptors
    Authors: MDM Fernández-, JM Grondona, P Fernández-, MD López-Ával
    J Neuroinflammation, 2019;16(1):245.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation
    Authors: M Yamamoto, M Kim, H Imai, Y Itakura, G Ohtsuki
    Cell Rep, 2019;28(11):2923-2938.e8.
    Species: Rat
    Sample Types: Tissue Culture Supernates
    Applications: Western Blot
  4. Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBP? in Alzheimer's Disease
    Authors: ZH Wang, J Xiang, X Liu, SP Yu, FP Manfredsso, IM Sandoval, S Wu, JZ Wang, K Ye
    Cell Rep, 2019;28(3):655-669.e5.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Andrographolide relieved pathological pain generated by spared nerve injury model in mice
    Authors: HC Wang, HS Tsay, HN Shih, YA Chen, KM Chang, DC Agrawal, S Huang, YL Lin, MJ Lee
    Pharm Biol, 2018;56(1):124-131.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  6. Anti-Inflammatory Action of Sitagliptin and Linagliptin in Doxorubicin Nephropathy
    Authors: CH Jo, S Kim, JS Park, GH Kim
    Kidney Blood Press. Res., 2018;43(3):987-999.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  7. Glial interleukin-1? upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats
    Authors: P Zhang, RY Bi, YH Gan
    J Neuroinflammation, 2018;15(1):117.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms
    Authors: L Taoro-Gonz, YM Arenas, A Cabrera-Pa, V Felipo
    J Neuroinflammation, 2018;15(1):36.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection
    Authors: HC Lai, CH Lu, CS Wong, BF Lin, SM Chan, CY Kuo, ZF Wu
    J Chin Med Assoc, 2018;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
  10. Anti-Inflammatory and Antinociceptive Effects of Ethyl Acetate Fraction of an Edible Red Macroalgae Sarcodia ceylanica
    Authors: CC Shih, HR Hwang, CI Chang, HM Su, PC Chen, HM Kuo, PJ Li, HD Wang, KH Tsui, YC Lin, SY Huang, ZH Wen
    Int J Mol Sci, 2017;18(11):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  11. Microglia-derived IL-1? promotes chemokine expression by M�ller cells and RPE in focal retinal degeneration
    Authors: R Natoli, N Fernando, M Madigan, JA Chu-Tan, K Valter, J Provis, M Rutar
    Mol Neurodegener, 2017;12(1):31.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  12. In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues
    Molecules, 2016;21(8):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. Inhibition of phagocytosis and pyroptosis of macrophages promotes Bartonella invasion into the bloodstream through lymphatic circulation
    Authors: Congli Yuan
    J. Infect. Dis., 2016;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia
    Authors: V Hernandez-, A Cabrera-Pa, L Taoro-Gonz, A Gonzalez-U, A Agusti, T Balzano, M Llansola, V Felipo
    J Neuroinflammation, 2016;13(1):83.
  15. The spinal anti-inflammatory mechanism of motor cortex stimulation: cause of success and refractoriness in neuropathic pain?
    Authors: Silva G, Lopes P, Fonoff E, Pagano R
    J Neuroinflammation, 2015;12(0):10.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  16. Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure.
    Authors: Madeira M, Elvas F, Boia R, Goncalves F, Cunha R, Ambrosio A, Santiago A
    J Neuroinflammation, 2015;12(0):115.
    Species: Rat
    Sample Types: Tissue Culture Supernates
    Applications: Neutralization
  17. Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration.
    Authors: Jiao H, Natoli R, Valter K, Provis J, Rutar M
    PLoS ONE, 2015;10(12):e0143952.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Spinal cord injury induces a long-lasting upregulation of interleukin-1beta in astrocytes around the central canal.
    Authors: Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, Molina-Holgado F, Garcia-Ovejero D
    Neuroscience, 2015;284(0):283-9.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  19. Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
    Authors: Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang T
    PLoS ONE, 2014;9(8):e106331.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
    Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K
    J Neurosci, 2014;34(6):2231-43.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration.
    Authors: Liu R, Gao J, Cao S, Sandhu N, Cui J, Chou C, Fang E, Matsubara J
    Invest Ophthalmol Vis Sci, 2013;54(3):2225-37.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
    J Neuroinflammation, 2012;9(0):181.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes.
    Authors: Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F
    J. Neurochem., 2011;117(1):164-74.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke.
    Authors: Faustino JV, Wang X, Johnson CE
    J. Neurosci., 2011;31(36):12992-3001.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  25. Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
    Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
    J. Neurochem., 2011;116(2):217-26.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
    Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J
    J. Clin. Invest., 2009;119(2):295-304.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
    Authors: Summy-Long JY, Hu S
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(5):R1532-45.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  28. Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
    Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
    Infect. Immun., 2008;76(8):3577-86.
    Species: Rat
    Sample Types: BALF
    Applications: ELISA Development
  29. Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland.
    Authors: Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A
    Endocrinology, 2008;149(4):1436-50.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  30. Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
    Authors: Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(3):H1839-46.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  31. IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.
    Authors: Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H
    Biochem. Biophys. Res. Commun., 2007;365(2):375-80.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  32. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
    Authors: Barkho BZ, Song H, Aimone JB
    Stem Cells Dev., 2006;15(3):407-21.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  33. In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
    Authors: Roche M, Diamond M, Finn DP
    J. Neuroimmunol., 2006;181(1):57-67.
    Species: Rat
    Sample Types: Plasma
    Applications: ELISA Development
  34. Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
    Authors: Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S
    Neuroscience, 2005;132(3):625-31.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines.
    Authors: Assmus M, Svechnikov K, von Euler M, Setchell B, Sultana T, Zetterstrom C, Holst M, Soder O
    Pediatr. Res., 2005;57(6):896-901.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  36. Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
    Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H
    J. Physiol. (Lond.), 2004;556(0):193-202.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  37. A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity.
    Authors: Guo L, LaDu MJ, Van Eldik LJ
    J. Mol. Neurosci., 2004;23(3):205-12.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Glucocorticoids play a fundamental role in protecting the brain during innate immune response.
    Authors: Nadeau S, Rivest S
    J. Neurosci., 2003;23(13):5536-44.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization

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Rat IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/07/2017
Application: ELISA Sample Tested: Serum Species: Rat

This polyclonal antibody was used as both a capture and detection (the biotinylated version of this antibody, BAF501, was used as the detection) in a sandwich ELISA. Rat IL-1b was successful measured in serum samples.