Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, ELISA Development, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL-1 beta /IL-1F2 (R&D Systems, Catalog # 501-RL)
Val117-Ser268
Accession # Q63264

Specificity

Detects rat IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL‑1 beta is observed and less than 0.5% cross-reactivity with recombinant human IL-1 beta and recombinant porcine IL-1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat IL‑1 beta /IL‑1F2 Antibody

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody.

Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND50 is typically 2-10 µg/mL.
IL‑1 beta /IL‑1F2 antibody in Rat Splenocytes by Immunocytochemistry (ICC).

IL‑1 beta /IL‑1F2 in Rat Splenocytes.

IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Flow Cytometry

Detection of Porcine IL-1 beta/IL-1F2 by Flow Cytometry

Correlation of CD206 and Il-1 beta immunolabelling within the CD11b+ macrophage population following LD.A: Representative flow cytometry plots examine CD206+ and Il1b+ cell counts within the CD11b population following light damage. For the most part, Il-1 beta and CD206 cells occupied mutually distinct subsets within the population CD11b cells. B: Quantification of Il-1 beta +/CD206- and CD206+/Il-1 beta - cells as percentage of the CD11b+ population following LD. There was a sharp increase in the proportion of Il-1 beta +/CD206- cells immediately following 24hrs LD (P<0.05), though this then decreased dramatically afterward and was similar to control samples by 7 days (P>0.05). For CD206+/Il-1 beta - cells, there was no change in their proportion at 24hrs LD (P>0.05). At 3 days post-exposure however the proportion of CD206+/Il-1 beta - cells had tripled (P<0.05), though this was then reduced to near control proportions by 7 days post-exposure (P<0.05). The trend of both Il-1 beta +/CD206- and CD206+/Il-1 beta - cells across the time course were significant by ANOVA (P < 0.05); N = 5 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Western Blot

Detection of Porcine IL-1 beta/IL-1F2 by Western Blot

The effect of chronic clomipramine treatment on the hippocampal NLRP3 inflammasome level in the CMS-treated rats.The protein levels of A IL-1 beta, B cleaved caspase-1, C NLRP3, D ASC, and E pro-caspase-1 (n = 4/group) were analyzed by western blot. All data are expressed as the mean ± SD. ##p < 0.01, ###p < 0.001 compared to saline-treated rats. *p < 0.05, **p <0.01, ***p < 0.001 compared to non-stressed control rats. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35688836), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Sustained IL-1 beta up-regulation in hippocampal astrocytes.IL-1 beta immunoreactivity was measured in GFAP-positive cells after LPS exposure or saline. Levels of IL-1 beta were found up-regulated from day 1 up to day 30. Higher magnification insets highlight the co-localization of IL-1 beta with GFAP (A). The ratio of IL-1 beta positive astrocytes in total astrocytes (GFAP positive cells) was quantified (B). Pictures show DG area, data are expressed as mean ± standard error of the mean (n = 4) and compared by 1-way analysis of variance followed with Boferroni post hoc analysis, **p<0.01, ***p<0.001 vs Control. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0106331), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Detection of Rat IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence

Sustained IL-1 beta up-regulation in hippocampal astrocytes.IL-1 beta immunoreactivity was measured in GFAP-positive cells after LPS exposure or saline. Levels of IL-1 beta were found up-regulated from day 1 up to day 30. Higher magnification insets highlight the co-localization of IL-1 beta with GFAP (A). The ratio of IL-1 beta positive astrocytes in total astrocytes (GFAP positive cells) was quantified (B). Pictures show DG area, data are expressed as mean ± standard error of the mean (n = 4) and compared by 1-way analysis of variance followed with Boferroni post hoc analysis, **p<0.01, ***p<0.001 vs Control. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0106331), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Rat IL‑1 beta /IL‑1F2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed LPS-stimulated rat splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL)

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2.

Rat IL-1 beta /IL-1F2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Rat IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF501)
  • Standard: Recombinant Rat IL-1 beta/IL-1F2 Protein (Catalog # 501-RL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF-501-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

Q63264

Additional IL-1 beta/IL-1F2 Products

Product Documents for Rat IL‑1 beta /IL‑1F2 Antibody

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  • Rat IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Rat
    Verified Customer | Posted 11/07/2017
    This polyclonal antibody was used as both a capture and detection (the biotinylated version of this antibody, BAF501, was used as the detection) in a sandwich ELISA. Rat IL-1b was successful measured in serum samples.
    Rat IL‑1 beta /IL‑1F2 Antibody AF-501-NA

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