Cytokine Immunoassay Kits

Immunoassays for Cytokine Secretion Analysis Products and Resources

Cytokine profiling and quantification are essential for characterizing immune responses, detecting disease-associated biomarkers, and monitoring responses to therapy. To support these studies, R&D Systems offers a wide selection of enzyme-linked immunosorbent assays (ELISAs) for cytokine analysis.

R&D Systems ELISA Kits provide the highest levels of performance and consistency in analyte quantification. Built using our own in-house antibodies, proteins, and diluents, each kit component and complete kit are carefully tested throughout development and manufacturing. This allows us to ensure that every R&D Systems ELISA meets our rigorous standards for specificity, recovery, linearity, and precision, so you can have confidence in your results from one experiment to the next.

Benefits of R&D Systems ELISAs

  • Most Referenced ELISA Manufacturer
  • Rigorous Validation Testing
  • Long-term Consistency
  • Exceptional Analyte Selection
  • Extensive Quality Control Testing
  • Bulk Packaging Available

Learn more about R&D Systems ELISA Quality Manufacturing and Validation Testing.

ELISA Kit Formats for Cytokine Profiling

We offer a broad range of single analyte immunoassay formats for cytokine quantification. These include our complete, ready-to-use Quantikine ELISA Kits, our 90-minute QuicKitELISAs, and the more versatile DuoSet ELISA Development Systems that provide all the components necessary for customers to develop their own working assays.

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Quantikine™ ELISA Kits

  • Extensively tested for long-term consistency and reproducibility
  • Fully validated sample types
  • Optimized reagents and diluents for accurate and sensitive sample values
Quantikine QuicKit™ ELISA Kits icon

Quantikine QuicKit ELISA Kits

  • Data generated in 90 minutes
  • Quantikine quality with lot-to-lot consistency and reproducibility
  • Simplified protocol – only one wash step
DuoSet™ ELISA Development Systems icon

DuoSet ELISA Development Systems

  • Carefully selected and validated matched antibody pairs for optimal performance
  • Large menu – over 1,000 targets with novel targets across multiple species
  • Adaptable for use across multiple platforms and 384 well

Select Single Analyte ELISAs for Chemokine Targets

 

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Select Single Analyte ELISAs for Interleukins/Interferons and their Soluble Receptors
TNF superfamily ligands, TNF-alpha, Fas Ligand, and TRAIL binding to their respective receptor complexes in the cell membrane

* Quantikine QuicKit ELISAs are available for these molecules.

R&D Systems Simple Reader Compact ELISA Plate Reader banner

ELISpot Kits and ELISpot Development Modules for Detecting Cytokine-Secreting Cells

ELISpot Kits are highly sensitive, microplate-based assays for the detection of cytokine-secreting cells. Complete ELISpot Kits are ready-to-run and require no assay development or refinement. As an alternative to our complete kits, we also offer ELISpot Development Modules, which provide a flexible, do-it-yourself format for ELISpot development.

  • Accurate quantitation – Small, crisp spots allow for simple and more quantitation and the largest dynamic range (number of detectable spots/well) on the market
  • High sensitivity – ELISpot Assays can measure responses with frequencies below 1 in 100,000 cells – up to 20% more sensitive than our competition
  • Assurance in the assay – We are the only company that provides recombinant protein positive controls

                        

IFN-gammaIgGIgMGranzyme BIL-2IL-4
IL-5IL-6IL-10IL-13IL-17TNF-alpha

Learn more about ELISpot Assays.

Multiplex Assays for Cytokine Analysis

Proteome Profiler Cytokine Antibody Arrays

Proteome Profiler Antibody Arrays are membrane-based arrays designed to simultaneous measure up to 119 proteins in a single sample. They provide a simple, economical, semi-quantitative multiplex assay for early stage discovery research. The arrays utilize chemiluminescence for detection, so membranes can be assessed for protein levels in the same manner as traditional Western blots.

R&D Systems Proteome Profiler Antibody Array assay principle
Human Chemokine Antibody Array31-plexCCL1 · CCL2 · CCL3/CCL4 · CCL5 · CCL7 · CCL14 · CCL15 · CCL17 · CCL18 · CCL19 · CCL20 · CCL21 · CCL22 · CCL26 · CCL28 · Chemerin · CX3CL1 · CXCL1 · CXCL4 · CXCL5 · CXCL7 · CXCL8 · CXCL9 · CXCL10 · CXCL11 · CXCL12 · CXCL16 · CXCL17 · IL-16 · Midkine · XCL1
Human Cytokine Antibody Array 36-plexC5a · CCL1 · CCL2 · CCL5 · CXCL1 · CXCL10 · CXCL11 · CXCL12 · CD40 Ligand · G-CSF · GM-CSF · ICAM-1 · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-4 · IL-5 · IL-6 · IL-8 · IL-10 · IL-12 p70 · IL-13 · IL-16 · IL-17 · IL-17E · IL-18 · IL-21 · IL-27 · IL-32alpha · MIF ·
MIP-1alpha/MIP-1beta · Serpin E1 · TNF-alpha · TREM-1
Human XL Cytokine Antbody Array105-plexAdiponectin · Angiogenin · Angiopoietin-1 · Angiopoietin-2 · Apolipoprotien A1 · BAFF · BDNF · C5a · CCL2 · CCL3/CCL4/MIP-1alpha/beta · CCL5 · CCL7 · CCL17 · CCL19 · CCL20 · CD14 · CD30 · CD31 · CD40 Ligand · Chitinase 3-like · Complement Factor D · C-Reactive Protein · Cripto-1 · CXCL1 · CXCL4 · CXCL5 · CXCL9 · CXCL10 · CXCL11 · CXCL12 · Cystatin C · Dkk-1 · DPPIV · EGF · Endoglin · EMMPRIN ·  Fas Ligand · FGF-basic · FGF-7/KGF ·FGF-19 · Flt-3 Ligand · G-CSF · GDF-15 · GM-CSF · Growth Hormone · HGF · ICAM-1 · IFN-gamma · IGFBP-2 · IGFBP-3 · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-3 · IL-4 · IL-5 · IL-6 · IL-8 · IL-10 · IL-11· IL-12 p70 · IL-13 · IL-15 · IL-16 · IL-17A · IL-18 BPa · IL-19 · IL-22 · IL-23 · IL-24 · IL-27 · IL-31 · IL-32alpha/beta/gamma · IL-33 · IL-34 · Kallikrein 3 · Leptin · LIF · Lipocalin-2 ·
M-CSF · MIF · MMP-9 · Myeloperoxidase · Osteopontin · PDGF-AA · PDGF-AB/BB · Pentraxin 3 · RAGE · RBP4 · Relaxin-2 · Resistin · Serpin E1 · SHBG · ST2 · TFF3 ·TfR · TGF-alpha · Thrombospondin-1 · TIM-1 · TNF-alpha · uPAR · VCAM-1 · VEGF · Vitamin D BP
Mouse Chemokine Antibody Array25-plexC5a · CCL2 · CCL3/CCL4 · CCL5 · CCL6/C10 · CCL8 · CCL9/10/MIP-1gamma · CCL11 · CCL12 · CCL21 · CCL22 · CCL27 · CCL28 · Chemerin · CX3CL1 · CXCL1 · CXCL2  · CXCL9 · CXCL10 · CXCL11 · CXCL12 · CXCL13 · CXCL16 · IL-16 · LIX
Mouse Cytokine Antibody Array40-plexC5a · CCL1 · CCL2 · CCL3 · CCL4 · CCL5 · CCL11 · CCL12 · CCL17 · CXCL1 · CXCL2 · CXCL9 · CXCL10 · CXCL11 · CXCL12 · CXCL13 · G-CSF · GM-CSF · ICAM-1 · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-3 · IL-4 · IL-5 · IL-6 · IL-7 · IL-10 · IL-12 p70 · IL-13 · IL-16 · IL-17 · IL-23 · IL-27 · M-CSF · TIMP-1 · TNF-alpha · TREM-1
Mouse XL Cytokine Antibody Array111-plexAdiponectin · Amphiregulin · Angiopoietin-1 · Angiopoietin-2 · Angiopoietin-like 3 · BAFF · C1q R1/CD93 · C5a · CCL2 · CCL3/CCL4/MIP-1alpha/beta · CCL5 · CCL6/C10 · CCL11 · CCL12 · CCL17 · CCL19 · CCL20 · CCL21 · CCL22 · CD14 · CD40 · CD160 · Chemerin · Chitinase 3-like · Coagulation Factor III/TF ·  Complement Factor D · C-Reactive Protein · CX3CL1 · CXCL1 · CXCL2 · CXCL9 · CXCL10 · CXCL11 · CXCL13 · CXCL16 ·Cystatin C · Dkk-1 · DPPIV · EGF · Endoglin · Endostatin · Fetuin A · FGF acidic · FGF-21 · Flt-3 Ligand · Gas6 · G-CSF · GDF-15 · GM-CSF · HGF · ICAM-1 · IFN-gamma · IGFBP-1 · IGFBP-2 · IGFBP-3 · IGFBP-5 · IGFBP-6 · IL-1alpha ·
IL-1beta · IL-1ra · IL-2 · IL-3 · IL-4 · IL-5 · IL-6 · IL-7 · IL-10 · IL-11· IL-12 p40 · IL-13 · IL-15 · IL-17A · IL-22 · IL-23 · IL-27 · IL-28 · IL-33 ·
LDL R · Leptin · LIF · Lipocalin-2 · LIX · M-CSF · MMP-2 · MMP-3 · MMP-9 · Myeloperoxidase · Osteopontin · Osteoprotegerin · PD-ECGF · PDGF-BB · Pentraxin 2 · Pentraxin 3 · Periostin · Pref-1 · Proliferin · Proprotein Convertase 9 · RAGE · RBP4 · Reg3G · Resistin ·
E-Selectin/CD62E · P-Selectin/CD62P · Serpin E1 · Serpin F1 · Thrombopoietin · TIM-1 · TNF-alpha · VCAM-1 · VEGF · WISP-1 

Learn more about Proteome Profiler Antibody Arrays.

Luminex® High Performance Cytokine Panels

Luminex panels are bead-based multiplex assays that enable you to multiplex up to 50 analytes, conserving on sample, time, and cost. These fully validated panels are our most accurate and precise bead-based multiplex assays for measuring target analytes in qualified complex sample types. They are our most accurate and precise bead-based multiplex assays for measuring target analytes in qualified complex sample types. All analytes in a given panel are optimized and tested together to ensure maximum performance and accuracy for the entire panel. Additionally, kits undergo similar validation testing as our single analyte Quantikine ELISA Kits and are correlated with our Quantikine ELISAs, when available.

Luminex High Performance Panels - Fixed

Fixed Luminex High Performance Panels provide a stocked and ready to ship option with shorter shipping times so you can begin your research more quickly.

Luminex® assay principle
Luminex High Performance Panels - FixedAnalytes
Human Chemokine Panel 8-plexEotaxin · GROalpha · IL-8 · IP-10 · MCP-1 · MIP-1alpha · MIP-1beta · RANTES
Human Cytokine Panel 15-plexIFN-alpha2 · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-3 · IL-4 · IL-6 · IL-7 · IL-9 · IL-10 · IL-15 · IL-33 · VEGF
Human Immunotherapy Panel 25-plexCD40 Ligand · GM-CSF · Granzyme B · IFN-alpha2 · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-4 · IL-6 · IL-8 · IL-9 · IL-10 · IL-12 p70 ·
IL-13 · IL-15 · IL-17A · IL-33 · IP-10 · MCP-1 · MIP-1alpha · MIP-1beta · PD-L1 · TNF-alpha
Human XL Cytokine Panel 46-plexCD40 Ligand · EGF · Eotaxin · FGF basic · Flt-3 Ligand · G-CSF · GM-CSF · Granzyme B · GRO alpha · GRO beta · IFN-alpha2 · IFN-beta · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-3 · IL-4 · IL-5 · IL-6 · IL-7 · IL-8 · IL-9 · IL-10 · IL-12 p70 · IL-13 · IL-15 · IL-17A · IL-17E · IL-33 · IP-10 · MCP-1 · MIP-1alpha · MIP-1beta · MIP-3alpha · MIP-3beta · PDGF-AA · PDGF-AB/BB · PD-L1 · RANTES · TGF-alpha · TNF-alpha · TNF-beta · TRAIL · VEGF
Human Th1/Th2 Panel 13-plexGM-CSF ·  IFN-gamma · IL-1beta · IL-2 · IL-4 · IL-5 · IL-6 · IL-9 · IL-10 · IL-12 p70 · IL-13 · TNF-alpha · TNF-beta
Human Th9/Th17/Th22 Panel 18-plexCD40 Ligand · GM-CSF · IFN-gamma· IL-1beta · IL-2 · IL-4 · IL-5 · IL-6 · IL-9 · IL-10 · IL-12 p70 · IL-13 · IL-15 · IL-17A · IL-17E · IL-33 · MIP-3alpha · TNF-alpha
Mouse XL Cytokine Panel 45-plexBAFF · CCL2 · CCL3 · CCL4 · CCL5 · CCL11 · CCL19 · Chitinase 3-like 1 · CXCL1 · CXCL10 · EGF · FGF basic · Flt-3 Ligand · G-CSF · GDF-15 ·
GM-CSF · ICAM-1 · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-3 · IL-4 · IL-5 · IL-6 · IL-7 · IL-9 · IL-10 · IL-11 · IL-12 p70 · IL-13 · IL-16 · IL-17A · IL-18 · IL-21 · IL-27 · IL-31 · LDL R · LIF · LIX · M-CSF · TIMP-1 · TNF-alpha · VEGF

 

Luminex High Performance Panels – Configurable

Configurable Luminex High Performance Panels allow users to choose from a selection of the analytes in the panel or the entire panel. They are supplied as either premixed or user-mixed panels as indicated on the kit specific product pages.                       

Luminex High Performance Panels - ConfigurableAnalytes
Human Cytokine Panel ACCL2 · CCL3 · CCL4 · CCL5 · CXCL5 · FGF basic · G-CSF · GM-CSF · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-4 · IL-5 · IL-6 · IL-8 ·
IL-10 · IL-17A · Thrombopoietin · TNF-alpha · VEGF
Human High Sensitivity Cytokine Panel AGM-CSF · IFN-gamma · IL-1beta · IL-2 · IL-4 · IL-5 · IL-6 · IL-8 · IL-10 · IL-12 · TNF-alpha · VEGF
Human High Sensitivity Cytokine Panel BGM-CSF · IFN-gamma · IL-1beta · IL-2  · IL-5 · IL-6 · IL-7 · IL-13 · IL-15 · IL-17A · IL-17F · IL-22 · IL-23 ·IL-31 · IL-33 · IL-36 beta · TNF-alpha 
Human XL Cytokine PanelCCL2 · CCL3 · CCL4 · CCL5 · CCL11 · CCL19 · CCL20 · CD40 Ligand · CXCL1 · CXCL2 · CXCL10 · EGF · FGF basic · Flt-3 Ligand · G-CSF ·
GM-CSF · Granzyme B · IFN-alpha2 · IFN-beta · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-3 · IL-4 · IL-5 · IL-6 · IL-7 · IL-8 · IL-9 · IL-10 · IL-12 p70 · IL-13 · IL-15 · IL-17A · IL-17E · IL-33 · PD-L1 · PDGF-AA · PDGF-AB/BB · TGF-alpha · TNF-alpha · TNF-beta · TRAIL · VEGF
Mouse XL Cytokine PanelBAFF · CCL2 · CCL3 · CCL4 · CCL5 · CCL11 · CCL19 · Chitinase 3-like 1 · CXCL1 · CXCL10 · EGF · FGF basic · Flt-3 Ligand · G-CSF ·
GDF-15 · GM-CSF · ICAM-1 · IFN-gamma · IL-1alpha · IL-1beta · IL-1ra · IL-2 · IL-3 · IL-4 · IL-5 · IL-6 · IL-7 · IL-9 · IL-10 · IL-11 · IL-12 p70 ·
IL-13 · IL-16 · IL-17A · IL-18 · IL-21 · IL-27 · IL-31 · LDL R · LIF · LIX · M-CSF · TIMP-1 · TNF-alpha · VEGF

Learn more about Luminex Assays.

Featured Content

R&D Systems ELISA Tips and Tricks poster

ELISA Tips and Tricks Poster

Learn tips on how to obtain consistent and reproducible data, ensure assay accuracy, and increase efficiency and speed.

R&D Systems ELISA Guide – A guide to running successful ELISAs

ELISA Guide

Explore our comprehensive guide to running a successful ELISA, including best practices, controls and troubleshooting.

R&D Systems Periodic Table of Cytokine and Chemokine Families poster

Periodic Table of Human Cytokine Families Poster

We love to make and measure cytokines and put them together in interesting ways. Check out our latest poster, it’s sure to brighten up your lab.

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