Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, urine and saliva.

Key Benefits

  • Detects 31 human chemokines simultaneously
  • Requires no specialized equipment
 

 

Principle of the Array

The Proteome Profiler Human Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Chemokines?

Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these chemokines in a single sample.
CCL1/I-309 CCL21/6Ckine CXCL8/IL-8
CCL2/MCP-1 CCL22/MDC CXCL9/MIG
CCL3/CCL4 (MIP-1 alpha/MIP-1 beta) CCL26/Eotaxin-3 CXCL10/IP-10
CCL5/RANTES CCL28 CXCL11/I-TAC
CCL7/MCP-3 Chemerin CXCL12/SDF-1
CCL14/HCC-1/HCC-3 CX3CL1/Fractalkine CXCL16
CCL15/MIP-1 delta/LKN-1 CXCL1/GRO alpha CXCL17/VCC-1
CCL17/TARC CXCL4/PF4 IL-16
CCL18/PARC CXCL5/ENA-78 Midkine
CCL19/MIP-3 beta CXCL7/NAP-2 XCL1/Lymphotactin
CCL20/MIP-3 alpha    

 

Assays for analytes represented in the Human Chemokine Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CCL1/I-309   DY272
CCL2/MCP-1 DCP00 DY279
CCL3/CCL4 (MIP-1 alpha/MIP-1 beta) DMA00/DMB00 DY270/DY271
CCL5/RANTES DRN00B DY278
CCL7/MCP-3 DCC700 DY282
CCL14/HCC-1/HCC-3   DY1578
CCL15/MIP-1 delta/LKN-1   DY363
CCL17/TARC DDN00 DY364
CCL18/PARC DCL180 DY394
CCL19/MIP-3 beta   DY361
CCL20/MIP-3 alpha DM3A00 DY360
CCL21/6Ckine   DY366
CCL22/MDC DMD00 DY336
CCL26/Eotaxin-3   DY346
CCL28   DY717
Chemerin DCHM00 DY2324
CX3CL1/Fractalkine DCX310 DY365
CXCL1/GRO alpha DGR00 DY275
CXCL4/PF4   DY795
CXCL5/ENA-78 DX000 DY254
CXCL7/NAP-2   DY393
CXCL8/IL-8 D8000C, HS800 DY208
CXCL9/MIG DCX900 DY392
CXCL10/IP-10 DIP100 DY266
CXCL11/I-TAC DCX110 DY672
CXCL12/SDF-1 DSA00 DY350
CXCL16 DCX160 DY1164
CXCL17/VCC-1    
IL-16 D1600 DY316
Midkine   DY258
XCL1/Lymphotactin   DY695

 

Data Examples
The Human Chemokine Array detects multiple analytes in cell culture supernates.
View Larger Image

The Human Chemokine Array detects multiple analytes in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 2 minute exposure to X-ray film.

Figure 1A: MG-63 human osteosarcoma cells were untreated or treated with 1 ng/mL recombinant human IL-1ß (R&D Systems, Catalog # 201-LB) for 72 hours.

THP-1 human acute monocytic leukemia cells
View Larger Image

Figure 1B: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, followed by the addition of 1 µg/mL LPS for 16 hours.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Species
Sample Type
  1. Indicators of responsiveness to immune checkpoint inhibitors
    Authors: BD Shields, F Mahmoud, EM Taylor, SD Byrum, D Sengupta, B Koss, G Baldini, S Ransom, K Cline, SG Mackintosh, RD Edmondson, S Shalin, AJ Tackett
    Sci Rep, 2017;7(1):807. 2017
  2. Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release
    Authors: S Giakoumelo, N Wheelhouse, J Brown, J Wade, I Simitsidel, D Gibson, PTK Saunders, P Horner, G Entrican, SEM Howie, AW Horne
    Sci Rep, 2017;7(1):2001. 2017
  3. Peri-foci adipose-derived stem cells promote chemoresistance in breast cancer
    Authors: WL Yeh, CF Tsai, DR Chen
    Stem Cell Res Ther, 2017;8(1):177. 2017
  4. Lysophosphatidic acid alters the expression profiles of angiogenic factors, cytokines, and chemokines in mouse liver sinusoidal endothelial cells.
    Authors: Chou C, Lai S, Ho C, Lin W, Chen C, Lee P, Peng F, Kuo S, Wu S, Lai H
    PLoS ONE, 2015;10(3):e0122060. 2015
  5. Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection.
    Authors: Marti H, Koschwanez M, Pesch T, Blenn C, Borel N
    PLoS ONE, 2014;9(7):e102239. 2014
  6. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
    Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
    Nat Commun, 2014;5(0):4715. 2014
  7. Age-related changes in expression and function of Toll-like receptors in human skin.
    Authors: Iram N, Mildner M, Prior M, Petzelbauer P, Fiala C, Hacker S, Schoppl A, Tschachler E, Elbe-Burger A
    Development, 2012;139(22):4210-9. 2012
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