The Proteome Profiler Human Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
|Simultaneously detect the levels of these chemokines in a single sample.|
|CCL3/CCL4 (MIP-1 alpha/MIP-1 beta)||CCL26/Eotaxin-3||CXCL10/IP-10|
|CCL15/MIP-1 delta/LKN-1||CXCL1/GRO alpha||CXCL17/VCC-1|
|Analyte||Quantikine® ELISA Kits||DuoSet® ELISA Development Systems|
|CCL3/CCL4 (MIP-1 alpha/MIP-1 beta)||DMA00/DMB00||DY270/DY271|
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The Human Chemokine Array detects multiple analytes in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 2 minute exposure to X-ray film.
Figure 1A: MG-63 human osteosarcoma cells were untreated or treated with 1 ng/mL recombinant human IL-1ß (R&D Systems, Catalog # 201-LB) for 72 hours.
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Figure 1B: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, followed by the addition of 1 µg/mL LPS for 16 hours.
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