Proteome Profiler Human Chemokine Array Kit

Contains 4 membranes - each spotted in duplicate with 31 different chemokine antibodies
Catalog # Availability Size / Price Qty
Detection of Multiple Analytes in Cell Culture Supernate with the Human Chemokine Array.
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Product Details
Citations (23)
Supplemental Products
Reviews (1)

Proteome Profiler Human Chemokine Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, urine and saliva.


Troubleshooting Guide

Key Benefits

  • Detects 31 human chemokines simultaneously
  • Requires no specialized equipment

Principle of the Array

The Proteome Profiler Human Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Chemokines?

Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.


Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.


Simultaneously detect the levels of these chemokines in a single sample.
CCL1/I-309 CCL21/6Ckine CXCL8/IL-8
CCL3/CCL4 (MIP-1 alpha/MIP-1 beta) CCL26/Eotaxin-3 CXCL10/IP-10
CCL7/MCP-3 Chemerin CXCL12/SDF-1
CCL14/HCC-1/HCC-3 CX3CL1/Fractalkine CXCL16
CCL15/MIP-1 delta/LKN-1 CXCL1/GRO alpha CXCL17/VCC-1
CCL18/PARC CXCL5/ENA-78 Midkine
CCL19/MIP-3 beta CXCL7/NAP-2 XCL1/Lymphotactin
CCL20/MIP-3 alpha    


Assays for analytes represented in the Human Chemokine Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CCL1/I-309   DY272
CCL2/MCP-1 DCP00 DY279
CCL3/CCL4 (MIP-1 alpha/MIP-1 beta) DMA00/DMB00 DY270/DY271
CCL7/MCP-3 DCC700 DY282
CCL14/HCC-1/HCC-3   DY1578
CCL15/MIP-1 delta/LKN-1   DY363
CCL19/MIP-3 beta   DY361
CCL20/MIP-3 alpha DM3A00 DY360
CCL21/6Ckine   DY366
CCL26/Eotaxin-3   DY346
CCL28   DY717
Chemerin DCHM00 DY2324
CX3CL1/Fractalkine DCX310 DY365
CXCL1/GRO alpha DGR00 DY275
CXCL4/PF4   DY795
CXCL5/ENA-78 DX000 DY254
CXCL7/NAP-2   DY393
CXCL8/IL-8 D8000C, HS800 DY208
CXCL10/IP-10 DIP100 DY266
CXCL12/SDF-1 DSA00 DY350
CXCL16 DCX160 DY1164
IL-16 D1600 DY316
Midkine   DY258
XCL1/Lymphotactin   DY695


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Scientific Data

Detection of Multiple Analytes in Cell Culture Supernate with the Human Chemokine Array. Cells were untreated or treated as indicated. 500 µL of cell culture supernate was run on each array. Data shown are from a 2 minute exposure to X-ray film. MG-63 human osteosarcoma cells were untreated or treated with 1 ng/mL recombinant human IL-1 beta (R&D Systems, Catalog # 201-LB) for 72 hours.

Detection of Multiple Analytes in Cell Culture Supernate with the Human Chemokine Array. Cells were untreated or treated as indicated. 500 µL of cell culture supernate was run on each array. Data shown are from a 2 minute exposure to X-ray film. THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, followed by the addition of 1 µg/mL LPS for 16 hours.

Citations for Proteome Profiler Human Chemokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Dissecting the Therapeutic Mechanisms of Sphingosine-1-Phosphate Receptor Agonism during Ischaemia and Reperfusion
    Authors: Wilkins, GC;Gilmour, J;Giannoudaki, E;Kirby, JA;Sheerin, NS;Ali, S;
    International journal of molecular sciences  2023-07-07
  2. Tspan6 stimulates the chemoattractive potential of breast cancer cells for B cells in an EV- and LXR-dependent manner
    Authors: G Molostvov, M Gachechila, AM Shaaban, S Hayward, I Dean, IHK Dias, N Badr, I Danial, F Mohammed, V Novitskaya, L Paniushkin, V Speirs, A Hanby, I Nazarenko, DR Withers, S van Laere, HM Long, F Berditchev
    Cell Reports, 2023-03-03;42(3):112207.  2023-03-03
  3. Insights into innate immune activation via PS-ASO-protein-TLR9 interactions
    Authors: AJ Pollak, L Zhao, TA Vickers, IJ Huggins, XH Liang, ST Crooke
    Nucleic Acids Research, 2022-08-12;0(0):.  2022-08-12
  4. A comprehensive profile of chemokines in the peripheral blood and vascular tissue of patients with Takayasu arteritis
    Authors: X Kong, S Wu, X Dai, W Yu, J Wang, Y Sun, Z Ji, L Ma, X Dai, H Chen, L Ma, L Jiang
    Arthritis Research & Therapy, 2022-02-16;24(1):49.  2022-02-16
  5. The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner
    Authors: R Schoppmeye, ACI van Steen, L Kempers, AL Timmerman, MA Nolte, P Hombrink, JD van Buul
    Cell Reports, 2022-01-18;38(3):110243.  2022-01-18
  6. TNF-alpha Activating Osteoclasts in Patients with Psoriatic Arthritis Enhances the Recruitment of Osteoclast Precursors: A Plausible Role of WNT5A-MCP-1 in Osteoclast Engagement in Psoriatic Arthritis
    Authors: SH Lin, JC Ho, SC Li, YW Cheng, CY Hsu, WY Chou, CC Hsiao, CH Lee
    International Journal of Molecular Sciences, 2022-01-15;23(2):.  2022-01-15
  7. Efficacy of chitinase-3-like protein 1 as an in�vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells
    Authors: N Komabashir, F Suehiro, M Ishii, M Nishimura
    Regenerative Therapy, 2021-03-27;18(0):38-50.  2021-03-27
  8. Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
    Authors: X Guo, Y Pan, M Xiong, S Sanapala, C Anastasaki, O Cobb, S Dahiya, DH Gutmann
    Nat Commun, 2020-05-01;11(1):2177.  2020-05-01
  9. Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells
    Authors: O Goncharova, N Flinner, J Bein, C Döring, E Donnadieu, S Rikirsch, M Herling, R Küppers, ML Hansmann, S Hartmann
    Cancers (Basel), 2019-10-02;11(10):.  2019-10-02
  10. Obesity in acute alcoholic hepatitis increases morbidity and mortality
    Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
    EBioMedicine, 2019-07-03;45(0):511-518.  2019-07-03
  11. A Novel Gastric Spheroid Co-Culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
    Authors: TA Sebrell, M Hashimi, B Sidar, R Wilkinson, L Kirpotina, MT Quinn, Z Malkoç, PJ Taylor, JN Wilking, D Bimczok
    Cell Mol Gastroenterol Hepatol, 2019-03-13;0(0):.  2019-03-13
  12. Host chemokine signature as a biomarker for the detection of pre-cancerous cervical lesions
    Authors: R Bhatia, K Kavanagh, J Stewart, S Moncur, I Serrano, D Cong, HA Cubie, JG Haas, C Busby-Earl, ARW Williams, SEM Howie, K Cuschieri
    Oncotarget, 2018-04-06;9(26):18548-18558.  2018-04-06
  13. Glycosylated extracellular vesicles released by glioblastoma cells are decorated by CCL18 allowing for cellular uptake via chemokine receptor CCR8
    Authors: J Berenguer, T Lagerweij, XW Zhao, S Dusoswa, P van der St, B Westerman, MC de Gooijer, M Zoetemelk, A Zomer, MHW Crommentui, LE Wedekind, À López-Lópe, A Giovanazzi, M Bruch-Oms, IH van der Me, RM Reijmers, TH van Kuppev, JJ García-Val, Y van Kooyk, BA Tannous, P Wesseling, D Koppers-La, WP Vandertop, DP Noske, VW van Beusec, J van Rheene, DM Pegtel, O van Tellin, T Wurdinger
    J Extracell Vesicles, 2018-03-13;7(1):1446660.  2018-03-13
  14. C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
    Authors: S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami
    Cancer Sci., 2018-02-14;109(3):724-731.  2018-02-14
  15. Papillary renal cell carcinoma-derived chemerin, IL-8, and CXCL16 promote monocyte recruitment and differentiation into foam-cell macrophages
    Authors: KM Krawczyk, H Nilsson, R Allaoui, D Lindgren, M Arvidsson, K Leandersso, ME Johansson
    Lab. Invest., 2017-07-31;97(11):1296-1305.  2017-07-31
  16. Peri-foci adipose-derived stem cells promote chemoresistance in breast cancer
    Authors: WL Yeh, CF Tsai, DR Chen
    Stem Cell Res Ther, 2017-07-27;8(1):177.  2017-07-27
  17. Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release
    Authors: S Giakoumelo, N Wheelhouse, J Brown, J Wade, I Simitsidel, D Gibson, PTK Saunders, P Horner, G Entrican, SEM Howie, AW Horne
    Sci Rep, 2017-05-17;7(1):2001.  2017-05-17
  18. Indicators of responsiveness to immune checkpoint inhibitors
    Authors: BD Shields, F Mahmoud, EM Taylor, SD Byrum, D Sengupta, B Koss, G Baldini, S Ransom, K Cline, SG Mackintosh, RD Edmondson, S Shalin, AJ Tackett
    Sci Rep, 2017-04-11;7(1):807.  2017-04-11
  19. Lysophosphatidic acid alters the expression profiles of angiogenic factors, cytokines, and chemokines in mouse liver sinusoidal endothelial cells.
    Authors: Chou C, Lai S, Ho C, Lin W, Chen C, Lee P, Peng F, Kuo S, Wu S, Lai H
    PLoS ONE, 2015-03-30;10(3):e0122060.  2015-03-30
  20. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
    Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
    Nat Commun, 2014-09-02;5(0):4715.  2014-09-02
  21. Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection.
    Authors: Marti H, Koschwanez M, Pesch T, Blenn C, Borel N
    PLoS ONE, 2014-07-14;9(7):e102239.  2014-07-14
  22. Moraxella catarrhalis adhesin UspA1-derived recombinant fragment rD-7 induces monocyte differentiation to CD14+CD206+ phenotype.
    Authors: Xie, Qi, Brackenbury, Louise S, Hill, Darryl J, Williams, Neil A, Qu, Xun, Virji, Mumtaz
    PLoS ONE, 2014-03-05;9(3):e90999.  2014-03-05
  23. Age-related changes in expression and function of Toll-like receptors in human skin.
    Authors: Iram N, Mildner M, Prior M, Petzelbauer P, Fiala C, Hacker S, Schoppl A, Tschachler E, Elbe-Burger A
    Development, 2012-10-03;139(22):4210-9.  2012-10-03


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Proteome Profiler Human Chemokine Array Kit
By Anonymous on 11/08/2020