Proteome Profiler Human Chemokine Array Kit
Proteome Profiler Human Chemokine Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, urine and saliva.
- Detects 31 human chemokines simultaneously
- Requires no specialized equipment
Principle of the Array
The Proteome Profiler Human Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Chemokines?
Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
|Simultaneously detect the levels of these chemokines in a single sample.|
|CCL3/CCL4 (MIP-1 alpha/MIP-1 beta)||CCL26/Eotaxin-3||CXCL10/IP-10|
|CCL15/MIP-1 delta/LKN-1||CXCL1/GRO alpha||CXCL17/VCC-1|
Assays for analytes represented in the Human Chemokine Array Kit
|Analyte||Quantikine® ELISA Kits||DuoSet® ELISA Development Systems|
|CCL3/CCL4 (MIP-1 alpha/MIP-1 beta)||DMA00/DMB00||DY270/DY271|
Detection of Multiple Analytes in Cell Culture Supernate with the Human Chemokine Array. Cells were untreated or treated as indicated. 500 µL of cell culture supernate was run on each array. Data shown are from a 2 minute exposure to X-ray film. MG-63 human osteosarcoma cells were untreated or treated with 1 ng/mL recombinant human IL-1 beta (R&D Systems, Catalog # 201-LB) for 72 hours.
Detection of Multiple Analytes in Cell Culture Supernate with the Human Chemokine Array. Cells were untreated or treated as indicated. 500 µL of cell culture supernate was run on each array. Data shown are from a 2 minute exposure to X-ray film. THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, followed by the addition of 1 µg/mL LPS for 16 hours.
Citations for Proteome Profiler Human Chemokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
Authors: X Guo, Y Pan, M Xiong, S Sanapala, C Anastasaki, O Cobb, S Dahiya, DH Gutmann
Nat Commun, 2020;11(1):2177. 2020
A Novel Gastric Spheroid Co-Culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
Authors: TA Sebrell, M Hashimi, B Sidar, R Wilkinson, L Kirpotina, MT Quinn, Z Malkoç, PJ Taylor, JN Wilking, D Bimczok
Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019
Obesity in acute alcoholic hepatitis increases morbidity and mortality
Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
EBioMedicine, 2019;45(0):511-518. 2019
Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells
Authors: O Goncharova, N Flinner, J Bein, C Döring, E Donnadieu, S Rikirsch, M Herling, R Küppers, ML Hansmann, S Hartmann
Cancers (Basel), 2019;11(10):. 2019
Host chemokine signature as a biomarker for the detection of pre-cancerous cervical lesions
Authors: R Bhatia, K Kavanagh, J Stewart, S Moncur, I Serrano, D Cong, HA Cubie, JG Haas, C Busby-Earl, ARW Williams, SEM Howie, K Cuschieri
Oncotarget, 2018;9(26):18548-18558. 2018
C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
Authors: S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami
Cancer Sci., 2018;109(3):724-731. 2018
Glycosylated extracellular vesicles released by glioblastoma cells are decorated by CCL18 allowing for cellular uptake via chemokine receptor CCR8
Authors: J Berenguer, T Lagerweij, XW Zhao, S Dusoswa, P van der St, B Westerman, MC de Gooijer, M Zoetemelk, A Zomer, MHW Crommentui, LE Wedekind, À López-Lópe, A Giovanazzi, M Bruch-Oms, IH van der Me, RM Reijmers, TH van Kuppev, JJ García-Val, Y van Kooyk, BA Tannous, P Wesseling, D Koppers-La, WP Vandertop, DP Noske, VW van Beusec, J van Rheene, DM Pegtel, O van Tellin, T Wurdinger
J Extracell Vesicles, 2018;7(1):1446660. 2018
Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release
Authors: S Giakoumelo, N Wheelhouse, J Brown, J Wade, I Simitsidel, D Gibson, PTK Saunders, P Horner, G Entrican, SEM Howie, AW Horne
Sci Rep, 2017;7(1):2001. 2017
Peri-foci adipose-derived stem cells promote chemoresistance in breast cancer
Authors: WL Yeh, CF Tsai, DR Chen
Stem Cell Res Ther, 2017;8(1):177. 2017
Papillary renal cell carcinoma-derived chemerin, IL-8, and CXCL16 promote monocyte recruitment and differentiation into foam-cell macrophages
Authors: KM Krawczyk, H Nilsson, R Allaoui, D Lindgren, M Arvidsson, K Leandersso, ME Johansson
Lab. Invest., 2017;97(11):1296-1305. 2017
Indicators of responsiveness to immune checkpoint inhibitors
Authors: BD Shields, F Mahmoud, EM Taylor, SD Byrum, D Sengupta, B Koss, G Baldini, S Ransom, K Cline, SG Mackintosh, RD Edmondson, S Shalin, AJ Tackett
Sci Rep, 2017;7(1):807. 2017
Lysophosphatidic acid alters the expression profiles of angiogenic factors, cytokines, and chemokines in mouse liver sinusoidal endothelial cells.
Authors: Chou C, Lai S, Ho C, Lin W, Chen C, Lee P, Peng F, Kuo S, Wu S, Lai H
PLoS ONE, 2015;10(3):e0122060. 2015
Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
Nat Commun, 2014;5(0):4715. 2014
Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection.
Authors: Marti H, Koschwanez M, Pesch T, Blenn C, Borel N
PLoS ONE, 2014;9(7):e102239. 2014
Age-related changes in expression and function of Toll-like receptors in human skin.
Authors: Iram N, Mildner M, Prior M, Petzelbauer P, Fiala C, Hacker S, Schoppl A, Tschachler E, Elbe-Burger A
Development, 2012;139(22):4210-9. 2012
Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?
The proteinase inhibitors are recommended for cell and tissue lysates only. You do not need them for cell culture supernates.
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