Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 612312
View all Bad Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bad
Met1-Gln168
Accession # Q92934

Specificity

Detects human Bad in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Bad (aa 1-130), rmBcl-2 alpha, recombinant human (rh) Bcl-2, or rhBCL2L12 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Bad Antibody

Detection of Human, Mouse, and Rat Bad antibody by Western Blot.

Detection of Human, Mouse, and Rat Bad by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Bad Monoclonal Antibody (Catalog # MAB6405) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bad at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Bad antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

Bad in Jurkat Human Cell Line.

Bad was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human Bad Monoclonal Antibody (Catalog # MAB6405) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Bad antibody in Human Kidney by Immunohistochemistry (IHC-P).

Bad in Human Kidney.

Bad was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Bad Monoclonal Antibody (Catalog # MAB6405) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot Shows Human Bad Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Bad Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bad knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bad Monoclonal Antibody (Catalog # MAB6405) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Bad at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Bad Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Knockout Validated

Bad is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Bad knockout HeLa cell line.

Western Blot

0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB6405 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bad

Bcl-2 antagonist of cell death (Bad) is a 23 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Human Bad shares 75% aa sequence identity with mouse and rat Bad.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Entrez Gene IDs

572 (Human); 12015 (Mouse); 64639 (Rat)

Gene Symbol

BAD

UniProt

Q92934 (Human)

Additional Bad Products

Product Documents for Human Bad Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Bad Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human Bad Antibody

Customer Reviews for Human Bad Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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Customer Images

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  • Human Bad Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Bad Antibody MAB6405
  • Human Bad Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 03/31/2018

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Protocols

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