Human NCAM-1/CD56 Antibody Summary
Leu20-Pro603 and Glu636-Asn741
Accession # NP_001070150
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human NCAM‑1/CD56 by Western Blot. Western blot shows lysates of human brain (cerebellum and hypothalamus). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB24081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NCAM‑1/CD56 at approximately 105-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of NCAM-1/CD56 in Human PBMC by Flow Cytometry. Human PBMCs were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NKp46 PE-conjugated Monoclonal Antibody (FAB1850P). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of NCAM-1/CD56 in Human NK cells by Flow Cytometry. Human NK cells were expanded from PBMCs using Cloudz Human NK Cell Expansion Kit (CLD004) and were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-HumanCD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol.
NCAM‑1/CD56 in Human Brain. NCAM-1/CD56 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) at 25 µg/mL over-night at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).
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- Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
- Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
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- Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
- Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
- Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
- Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
- Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
- Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
- Doherty, P. et al. (1995) J. Neurobiol. 26:437.
- Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
- Muller, D. et al. (1996) Neuron 17:413.
Citations for Human NCAM-1/CD56 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey
Authors: X Wang, M Lu, X Tian, Y Ren, Y Li, M Xiang, S Chen
Stem Cell Res Ther, 2020;11(1):334.
Sample Types: Protein
Radiotherapy and chemotherapy change vessel tree geometry and metastatic spread in a small cell lung cancer xenograft mouse tumor model
Authors: T Frenzel, B Hoffmann, R Schmitz, A Bethge, U Schumacher, G Wedemann
PLoS ONE, 2017;12(11):e0187144.
Sample Types: Whole Tissue
Selectins mediate small cell lung cancer systemic metastasis.
Authors: Heidemann F, Schildt A, Schmid K, Bruns O, Riecken K, Jung C, Ittrich H, Wicklein D, Reimer R, Fehse B, Heeren J, Luers G, Schumacher U, Heine M
PLoS ONE, 2014;9(4):e92327.
Sample Types: Cell Culture Supernates
Applications: Flow Cytometry
Satellite cells senescence in limb muscle of severe patients with COPD.
Authors: Theriault ME, Pare MÃˆ, Maltais F, Debigare R
PLoS ONE, 2012;7(6):e39124.
Sample Types: Whole Tissue
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