Human NKp46/NCR1 Antibody

Clone 195314 was used by HLDA to establish CD designation.
(13 citations)
(4 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human NKp46/NCR1 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 195314
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse T cell hybridoma transfected with human NKp46/NCR1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human NKp46/NCR1 Fc Chimera (Catalog # 1850-NK)
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood CD56+ natural killer cells
  • Agonist Activity
    Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells.
    The ED50 for this effect is typically 0.1‑0.3 μg/mL.
  • CyTOF-reported
    Horowitz, A. et al. Sci. Transl. Med. (2013) 208ra145. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
NKp46/NCR1 in NK‑92 Human Cell Line. NKp46/NCR1 was detected in immersion fixed NK‑92 human natural killer lymphoma cell line using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255. 
    2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. 
    4. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    5. Arnon, T. et al. (2004) Blood 103:664.
    6. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
    7. Mandelboim, O. et al. (2001) Nature 409:1055.
    8. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.
  • Entrez Gene IDs:
    9437 (Human); 17086 (Mouse)
  • Alternate Names:
    CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
    Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
    Sci Rep, 2017;7(0):44544.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016;197(5):1621-30.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection.
    Authors: Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z
    Immunity, 2016;45(1):131-44.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  4. Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients.
    Authors: Messaoudene M, Perier A, Fregni G, Neves E, Zitvogel L, Cremer I, Chanal J, Sastre-Garau X, Deschamps L, Marinho E, Larousserie F, Maubec E, Avril M, Caignard A
    PLoS ONE, 2015;10(7):e0133363.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  5. A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.
    Authors: Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss S, Kiprijanov S, Little M, Zhukovsky E
    MAbs, 2014;6(3):728-39.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  6. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
    Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
    J Immunol, 2014;192(8):3805-15.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  7. Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1).
    Authors: Durrenberger PF, Ettorre A, Kamel F
    J Neuroinflammation, 2012;9(0):1.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients.
    Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F
    PLoS ONE, 2012;7(11):e49135.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Sequential metastases of colorectal cancer: Immunophenotypes and spatial distributions of infiltrating immune cells in relation to time and treatments.
    Authors: Keim S, Zoernig I, Spille A, Lahrmann B, Brand K, Herpel E, Grabe N, Jager D, Halama N
    Oncoimmunology, 2012;1(5):593-599.
  10. NKp46 identifies an NKT cell subset susceptible to leukemic transformation in mouse and human.
    Authors: Yu J, Mitsui T, Wei M, Mao H, Butchar JP, Shah MV, Zhang J, Mishra A, Alvarez-Breckenridge C, Liu X, Liu S, Yokohama A, Trotta R, Marcucci G, Benson DM, Loughran TP, Tridandapani S, Caligiuri MA
    J. Clin. Invest., 2011;121(4):1456-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  11. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008;181(3):2227-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  12. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007;111(3):1309-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. A novel binding assay to assess specificity of monoclonal antibodies.
    Authors: Warren HS, Warren&lt;/LastName&gt;&lt;ForeNam HS&lt;/Initia, Bettadapura J
    J. Immunol. Methods, 2005;305(1):33-8.
    Species: Human
    Sample Type: Complex Sample Type
    Application: Epitope Mapping
Expand to show all 13 Citations
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Average Rating: 3.8 (Based on 4 reviews)

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We have 4 reviews tested in 1 species: Human.
We have 4 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human NKp46/NCR1 Antibody MAB1850
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Average
 ICC/IF Human Anonymous 10/01/2017
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Immunocytochemistry/Immunofluorescence Human NKp46/NCR1 Antibody MAB1850
Immunocytochemistry/Immunofluorescence: Human NKp46/NCR1 Antibody [MAB1850]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedKidney (cortex) tissue
SpeciesHuman
Immunohistochemistry Human NKp46/NCR1 Antibody MAB1850
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Very Good
 IHC Human Anonymous 10/26/2015
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Immunohistochemistry Human NKp46/NCR1 Antibody MAB1850
Immunohistochemistry: Human NKp46/NCR1 Antibody [MAB1850]

Summary

ApplicationImmunohistochemistry
Sample TestedColon tissue
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAt this concentration there is little backgroud.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:500
Immunohistochemistry Human NKp46/NCR1 Antibody MAB1850
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Very Good
 IHC Human Anonymous 10/26/2015
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Immunohistochemistry Human NKp46/NCR1 Antibody MAB1850
Immunohistochemistry: Human NKp46/NCR1 Antibody [MAB1850]

Summary

ApplicationImmunohistochemistry
Sample TestedColon tissue
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSeems like the immune cells show a little staining. At 1:250 all cells showed staining. Dilutions are important.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000
Immunocytochemistry/Immunofluorescence Human NKp46/NCR1 Antibody MAB1850
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Very Good
 ICC/IF Human Anonymous 10/26/2015
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Image Details
Immunocytochemistry/Immunofluorescence Human NKp46/NCR1 Antibody MAB1850
Immunocytochemistry/Immunofluorescence: Human NKp46/NCR1 Antibody [MAB1850]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedNatural killer cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsHuman NKp46/NCR1 MAb (Clone 195314), works well for detection of NKp46 on surface of NK cells.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:500

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