Human NKp46/NCR1 Antibody

Clone 195314 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1850-SP
MAB1850-100
MAB1850-500
NKp46/NCR1 in NK-92 Human Cell Line and Human PBMCs.
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Product Details
Citations (27)
FAQs
Supplemental Products
Reviews (6)

Human NKp46/NCR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKp46/NCR1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 195314
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse T cell hybridoma transfected with human NKp46/NCR1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human NKp46/NCR1 Fc Chimera (Catalog # 1850-NK)
Flow Cytometry
0.25 µg/106 cells
Human whole blood CD56+ natural killer cells
Immunohistochemistry
5-25 µg/mL
See below
Agonist Activity
Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells.
The ED50 for this effect is typically ≤ 1 μg/mL.
 
CyTOF-reported
Horowitz, A. et al. Sci. Transl. Med. (2013) 208ra145. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry NKp46/NCR1 in NK-92 Human Cell Line and Human PBMCs. View Larger

NKp46/NCR1 in NK-92 Human Cell Line and Human PBMCs. NKp46/NCR1 was detected in immersion fixed NK-92 human natural killer lymphoma cell line (left panel, positive stain) and human peripheral blood mononuclear cells (PBMCs; right panel, negative stain) using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry NKp46/NCR1 in Human Tonsil. View Larger

NKp46/NCR1 in Human Tonsil. NKp46/NCR1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to natural killer cells in germinal center. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow Cytometry Detection of NKp46/NCR1 in Human PBMCs by Flow Cytometry. View Larger

Detection of NKp46/NCR1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Rabbit Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

References
  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255. 
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. 
  4. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  5. Arnon, T. et al. (2004) Blood 103:664.
  6. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  7. Mandelboim, O. et al. (2001) Nature 409:1055.
  8. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.
Entrez Gene IDs
9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)
Alternate Names
CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

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Citations for Human NKp46/NCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Establishment of an efficient ex�vivo expansion strategy for human natural killer cells stimulated by defined cytokine cocktail and antibodies against natural killer cell activating receptors
    Authors: T Nakazawa, T Morimoto, R Maeoka, R Matsuda, M Nakamura, F Nishimura, S Yamada, I Nakagawa, YS Park, H Nakase, T Tsujimura
    Regenerative Therapy, 2022;21(0):185-191.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture
  2. Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion
    Authors: JA Myers, D Schirm, L Bendzick, R Hopps, C Selleck, P Hinderlie, M Felices, JS Miller
    JCI Insight, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  3. Flow cytometric single cell-based assay to simultaneously detect cell death, cell cycling, DNA content and cell senescence
    Authors: E Lieschke, Z Wang, C Chang, CE Weeden, GL Kelly, A Strasser
    Cell Death and Differentiation, 2022;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8
    Authors: T Walle, JA Kraske, B Liao, B Lenoir, C Timke, E von Bohlen, F Tran, P Griebel, D Albrecht, A Ahmed, M Suarez-Car, A Jiménez-Sá, T Beikert, A Tietz-Dahl, AN Menevse, G Schmidt, M Brom, JHW Pahl, W Antonopoul, M Miller, RL Perez, F Bestvater, NA Giese, P Beckhove, P Rosenstiel, D Jäger, O Strobel, D Pe'er, N Halama, J Debus, A Cerwenka, PE Huber
    Science Advances, 2022;8(12):eabh4050.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Wilms' Tumor Primary Cells Display Potent Immunoregulatory Properties on NK Cells and Macrophages
    Authors: PF Fiore, P Vacca, N Tumino, F Besi, A Pelosi, E Munari, M Marconi, I Caruana, V Pistoia, L Moretta, B Azzarone
    Cancers, 2021;13(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma
    Authors: O Melaiu, M Chierici, V Lucarini, G Jurman, LA Conti, R De Vito, R Boldrini, L Cifaldi, A Castellano, C Furlanello, V Barnaba, F Locatelli, D Fruci
    Nat Commun, 2020;11(1):5992.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
    Authors: JW Choi, S Lim, JH Kang, SH Hwang, KC Hwang, SW Kim, S Lee
    Molecules, 2020;25(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer
    Authors: K Yamashita, M Iwatsuki, N Yasuda-Yos, T Morinaga, Y Nakao, K Harada, K Eto, J Kurashige, Y Hiyoshi, T Ishimoto, Y Nagai, S Iwagami, Y Baba, Y Miyamoto, N Yoshida, JA Ajani, H Baba
    Br J Cancer, 2020;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition
    Authors: WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu
    Nature Neuroscience, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Mutated Von Hippel-Lindau-renal cell carcinoma (RCC) promotes patients specific natural killer (NK) cytotoxicity
    Authors: AM Trotta, S Santagata, S Zanotta, C D'Alterio, M Napolitano, G Rea, R Camerlingo, F Esposito, E Lamantia, A Anniciello, G Botti, N Longo, G Botti, S Pignata, S Perdonà, S Scala
    J. Exp. Clin. Cancer Res., 2018;37(1):297.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Targeted therapies for targeted populations: Anti-EGFR treatment for EGFR amplified gastroesophageal adenocarcinoma
    Authors: SB Maron, L Alpert, HA Kwak, S Lomnicki, L Chase, D Xu, E O'Day, RJ Nagy, RB Lanman, F Cecchi, T Hembrough, A Schrock, J Hart, SY Xiao, N Setia, DVT Catenacci
    Cancer Discov, 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models
    Authors: Y Feng, S Liao, C Wei, D Jia, K Wood, Q Liu, X Wang, FD Shi, WN Jin
    J Neuroinflammation, 2017;14(1):248.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
    Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
    Sci Rep, 2017;7(0):44544.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection.
    Authors: Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z
    Immunity, 2016;45(1):131-44.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. NKp30 isoforms and NKp46 transcripts in metastatic melanoma patients: Unique NKp30 pattern in rare melanoma patients with favorable evolution
    Authors: M Messaouden, G Fregni, D Enot, N Jacquelot, E Neves, N Germaud, HJ Garchon, W Boukouaci, R Tamouza, J Chanal, MF Avril, A Toubert, L Zitvogel, S Rusakiewic, A Caignard
    Oncoimmunology, 2016;5(12):e1154251.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  16. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016;197(5):1621-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients.
    Authors: Messaoudene M, Perier A, Fregni G, Neves E, Zitvogel L, Cremer I, Chanal J, Sastre-Garau X, Deschamps L, Marinho E, Larousserie F, Maubec E, Avril M, Caignard A
    PLoS ONE, 2015;10(7):e0133363.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.
    Authors: Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss S, Kiprijanov S, Little M, Zhukovsky E
    MAbs, 2014;6(3):728-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  19. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
    Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
    J Immunol, 2014;192(8):3805-15.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1).
    Authors: Durrenberger PF, Ettorre A, Kamel F
    J Neuroinflammation, 2012;9(0):1.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients.
    Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F
    PLoS ONE, 2012;7(11):e49135.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Sequential metastases of colorectal cancer: Immunophenotypes and spatial distributions of infiltrating immune cells in relation to time and treatments.
    Authors: Keim S, Zoernig I, Spille A, Lahrmann B, Brand K, Herpel E, Grabe N, Jager D, Halama N
    Oncoimmunology, 2012;1(5):593-599.
  23. NKp46 identifies an NKT cell subset susceptible to leukemic transformation in mouse and human.
    Authors: Yu J, Mitsui T, Wei M, Mao H, Butchar JP, Shah MV, Zhang J, Mishra A, Alvarez-Breckenridge C, Liu X, Liu S, Yokohama A, Trotta R, Marcucci G, Benson DM, Loughran TP, Tridandapani S, Caligiuri MA
    J. Clin. Invest., 2011;121(4):1456-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  24. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008;181(3):2227-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  25. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007;111(3):1309-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. A novel binding assay to assess specificity of monoclonal antibodies.
    Authors: Warren HS, Warren&lt;/LastName&gt;&lt;ForeNam HS&lt;/Initia, Bettadapura J
    J. Immunol. Methods, 2005;305(1):33-8.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Epitope Mapping
  27. Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways.
    Authors: Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, Hong M, Bertoletti A, Bicciato S, Invernizzi P, Lugli E, Torzilli G, Gershwin M, Mavilio D
    J Autoimmun, 0;66(0):40-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human NKp46/NCR1 Antibody

Average Rating: 4 (Based on 6 Reviews)

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Human NKp46/NCR1 Antibody
By Anonymous on 08/09/2021
Application: ICC/IF Sample Tested: Natural killer cells Species: Human

NKp46/NCR1 in NK-92 human natural killer lymphoma cell line


Human NKp46/NCR1 Antibody
By Anonymous on 05/18/2018
Application: IHC Sample Tested: Lymph node tissue Species: Human

Paraffin embedded lymph node metastasis samples
Antigen retrieval: Citrat buffer
Antibody Dilution: 1:50
Buffer: TBS-T

At this concentration, the isotype showed no specific staining, whereas MAB1850 showed clear surface staining of cells.


Human NKp46/NCR1 Antibody
By Becker Law on 10/01/2017
Application: ICC/IF Sample Tested: Kidney (cortex) tissue Species: Human

Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

At this concentration there is little backgroud.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:500


Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

Seems like the immune cells show a little staining. At 1:250 all cells showed staining. Dilutions are important.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000


Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: Natural killer cells Species: Human

Human NKp46/NCR1 MAb (Clone 195314), works well for detection of NKp46 on surface of NK cells.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:500