Human/Primate VEGF Antibody

  (39 citations)
(2 Reviews)
    
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Product Details
Citations (39)
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  • Species Reactivity
    Human, Primate
  • Specificity
    Detects human VEGF165 and human VEGF121 in ELISAs and Western blots. In ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and recombinant rat (rr)VEGF and no cross-reactivity with recombinant human (rh) VEGF-D is obseved.
  • Source
    Monoclonal Mouse IgG2B Clone # 26503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165
    Ala27-Arg191
    Accession # NP_001165097.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
    • Human/Primate VEGF Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Primate VEGF Antibody (Catalog # MAB293 )
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate VEGF165 Biotinylated Antibody (Catalog # BAF293 )
  • ELISA Standard
     
    Recombinant Human VEGF 165 Protein (Catalog # 293-VE )
  • Neutralization
    Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-60 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human VEGF by Western Blot. Western blot shows 25 ng of Recombinant Human VEGF165 (Catalog # 293‑VE), Recombinant Human VEGF111 (Catalog # 5336-VE), Recombinant Human VEGF121, aa 207-327 (Catalog # 4644-VS), Recombinant Human VEGF145 (aa 27-171) (Catalog # 7626‑VE), Recombinant Human VEGF162 (Catalog # 2347-VE), Recombinant Human VEGF165b (Catalog # 3045‑VE), Recombinant Human VEGF189 (aa 27-215) (Catalog # 8147‑VE), Recombinant Human VEGF165 Extended Isoform (Catalog # 9018‑VE), Recombinant Human VEGF‑B167 (Catalog # 751‑VE), Recombinant Mouse VEGF164 (Catalog # 493‑MV), and Recombinant Rat VEGF164 (Catalog # 564‑RV). PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VEGF at approximately 15-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293). The ND50 is typically 10-60 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF
VEGF is a soluble protein secreted by a wide variety of cell types. It binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (Flk-1). VEGF stimulates vascular endothelial cell proliferation and is a potent inducer of angiogenesis. Several VEGF isoforms occur resulting from alternative mRNA splicing.
  • Long Name:
    Vascular Endothelial Growth Factor
  • Entrez Gene IDs:
    7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
  • Alternate Names:
    MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells
    Authors: W Katagiri, T Kawai, M Osugi, Y Sugimura-W, K Sakaguchi, T Kojima, T Kobayashi
    Maxillofac Plast Reconstr Surg, 2017;39(1):8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Vessel morphometric parameters-correlation with histologic grade and VEGF expression in oligodendroglioma
    Authors: LB Strickland, S Brem, AM Rojiani, MV Rojiani
    Am J Cancer Res, 2017;7(4):973-981.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  4. Development of a surrogate potency assay to determine the angiogenic activity of Stempeucel�, a pooled, ex-vivo expanded, allogeneic human bone marrow mesenchymal stromal cell product
    Authors: C Thej, B Ramadasse, A Walvekar, AS Majumdar, S Balasubram
    Stem Cell Res Ther, 2017;8(1):47.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms
    Authors: Lopa Leach
    Placenta, 2016;48(0):87-98.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. VEGF-A acts via neuropilin-1 to enhance epidermal cancer stem cell survival and formation of aggressive and highly vascularized tumors
    Authors: D Grun, G Adhikary, RL Eckert
    Oncogene, 2016;35(33):4379-87.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer
    Authors: Z Jing, H Xu, X Chen, Q Zhong, J Huang, Y Zhang, W Guo, Z Yang, S Ding, P Chen, Z Huang
    PLoS ONE, 2016;11(4):e0152789.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
    Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T
    J Biol Chem, 2015;290(36):22212-24.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  9. Pericytes contribute to the disruption of the cerebral endothelial barrier via increasing VEGF expression: implications for stroke.
    Authors: Bai Y, Zhu X, Chao J, Zhang Y, Qian C, Li P, Liu D, Han B, Zhao L, Zhang J, Buch S, Teng G, Hu G, Yao H
    PLoS ONE, 2015;10(4):e0124362.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1alpha.
    Authors: Tan J, Prosser H, Vanags L, Monger S, Ng M, Bursill C
    FASEB J, 2014;28(1):206-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Control of angiogenesis by galectins involves the release of platelet-derived proangiogenic factors.
    Authors: Etulain J, Negrotto S, Tribulatti M, Croci D, Carabelli J, Campetella O, Rabinovich G, Schattner M
    PLoS ONE, 2014;9(4):e96402.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Heat shock protein 27 attenuates neointima formation and accelerates reendothelialization after arterial injury and stent implantation: importance of vascular endothelial growth factor up-regulation.
    Authors: Ma X, Hibbert B, McNulty M, Hu T, Zhao X, Ramirez F, Simard T, de Belleroche J, O'Brien E
    FASEB J, 2014;28(2):594-602.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
    Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
    Nat Commun, 2014;5(0):4715.
    Species: Human
    Sample Type: In Vivo
    Application: Neut
  14. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013;8(12):e85147.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Markers of T cell infiltration and function associate with favorable outcome in vascularized high-grade serous ovarian carcinoma.
    Authors: Townsend, Katelin, Spowart, Jaeline, Huwait, Hassan, Eshragh, Sima, West, Nathan R, Elrick, Mary A, Kalloger, Steve E, Anglesio, Michael, Watson, Peter H, Huntsman, David G, Lum, Julian J
    PLoS ONE, 2013;8(12):e82406.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo.
    Authors: Merdzhanova G, Gout S, Keramidas M, Edmond V, Coll JL, Brambilla C, Brambilla E, Gazzeri S, Eymin B
    Oncogene, 2010;29(39):5392-403.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  17. Complementary actions of inhibitors of angiopoietin-2 and VEGF on tumor angiogenesis and growth.
    Authors: Hashizume H, Falcon BL, Kuroda T, Baluk P, Coxon A, Yu D, Bready JV, Oliner JD, McDonald DM
    Cancer Res., 2010;70(6):2213-23.
    Species: Human
    Sample Type: In Vivo
    Application: In vivo
  18. A small-molecule triptolide suppresses angiogenesis and invasion of human anaplastic thyroid carcinoma cells via down-regulation of the nuclear factor-kappa B pathway.
    Authors: Zhu W, Ou Y, Li Y, Xiao R, Shu M, Zhou Y, Xie J, He S, Qiu P, Yan G
    Mol. Pharmacol., 2009;75(4):812-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  19. FGF5 as an oncogenic factor in human glioblastoma multiforme: autocrine and paracrine activities.
    Authors: Allerstorfer S, Sonvilla G, Fischer H, Spiegl-Kreinecker S, Gauglhofer C, Setinek U, Czech T, Marosi C, Buchroithner J, Pichler J, Silye R, Mohr T, Holzmann K, Grasl-Kraupp B, Marian B, Grusch M, Fischer J, Micksche M, Berger W
    Oncogene, 2008;27(30):4180-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  20. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Type: Serum
    Application: ELISA Microarray Development
  21. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  22. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  23. Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment.
    Authors: Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA
    Blood, 2007;109(1):122-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  24. Increased vascular endothelial growth factor and receptors: relationship to angiogenesis in asthma.
    Authors: Feltis BN, Wignarajah D, Zheng L, Ward C, Reid D, Harding R, Walters EH
    Am. J. Respir. Crit. Care Med., 2006;173(11):1201-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  25. The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
    Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
    Clin. Sci., 2006;110(5):575-85.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  26. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006;119(10):2313-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  27. Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice.
    Authors: Rofstad EK, Mathiesen B, Kindem K, Galappathi K
    Cancer Res., 2006;66(13):6699-707.
    Species: Complex Species Category
    Sample Type: In Vivo
    Application: In vivo
  28. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006;169(4):1167-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  29. Evaluating hypoxia-inducible factor-1alpha as a cancer therapeutic target via inducible RNA interference in vivo.
    Authors: Li L, Lin X, Staver M, Shoemaker A, Semizarov D, Fesik SW, Shen Y
    Cancer Res., 2005;65(16):7249-58.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  30. Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension.
    Authors: Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO
    Am. J. Physiol. Heart Circ. Physiol., 2005;290(3):H978-84.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Frozen
  31. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  32. Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
    Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
    Cancer Res., 2005;65(16):7241-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  33. Possible mechanisms inducing glomerulations in interstitial cystitis: relationship between endoscopic findings and expression of angiogenic growth factors.
    Authors: Tamaki M, Saito R, Ogawa O, Yoshimura N, Ueda T
    J. Urol., 2004;172(3):945-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  34. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003;33(10):883-90.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  35. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
    Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
    Cancer Res., 2003;63(4):887-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  36. Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
    Authors: Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
    Am. J. Pathol., 2002;160(4):1405-23.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  37. Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts.
    Authors: Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, Endoh M, Takamoto S, Amano J, Isobe M
    Transplant. Proc., 2000;32(5):987-9.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  38. Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
    Authors: Gu JW, Adair TH
    Am. J. Physiol., 1997;273(2):H628-33.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  39. VEGF in the lung: a role for novel isoforms.
    Authors: Varet, Julia, Varet J, Douglas SK, Douglas, Samantha, Gilmartin, Laura, Gilmartin L, Medford, Andrew R, Bates, David O, Harper, Steven J, Millar, Ann B
    Am J Physiol Lung Cell Mol Physiol, 0;298(6):L768-74.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
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Isotype Controls
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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Primary Antibodies
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Human/Primate VEGF Antibody

WB , IHC , Neut MAB293R  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 2 species: Hamster, Human.
We have 2 review tested in 1 application: ELISA.

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  Very Good
 ELISA Hamster Anonymous 11/04/2017
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Summary

Application ELISA
Sample Tested CHO Chinese hamster ovary cell line
Species Hamster
ELISA Human/Primate VEGF Antibody MAB293 Array MAB293
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Excellent
 ELISA Human Anonymous 08/18/2017
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ELISA Human/Primate VEGF Antibody MAB293
ELISA: Human/Primate VEGF Antibody [MAB293].

Summary

Application ELISA
Sample Tested THP-1 human acute monocytic leukemia cell line
Species Human

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