Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Mouse, Rat, Porcine, Bovine, Complex Species Category, Primate, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Array Development, Bioassay, ELISA Detection, ELISA Development, ELISA Microarray Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 26503
View all VEGF Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165
Ala27-Arg191
Accession # NP_001165097.1

Specificity

Detects human VEGF

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 µg of the antibody by the LAL method.

Scientific Data Images for Human/Primate VEGF Antibody

Detection of Recombinant Human VEGF antibody by Western Blot.

Detection of Recombinant Human VEGF by Western Blot.

Western blot shows 25 ng of Recombinant Human VEGF165 (293-VE), Recombinant Human VEGF111 (5336-VE), Recombinant Human VEGF121, aa 207-327 (4644-VS), Recombinant Human VEGF145 (aa 27-171) (7626-VE), Recombinant Human VEGF162 (2347-VE), Recombinant Human VEGF165b (3045-VE), Recombinant Human VEGF189 (aa 27-215) (8147-VE), Recombinant Human VEGF165 Extended Isoform (9018-VE), Recombinant Human VEGF-B167 (751-VE), Recombinant Mouse VEGF164 (493-MV), and Recombinant Rat VEGF164 (564-RV). PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for VEGF at approximately 15-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by VEGF165and Neutralization by Human VEGF Antibody.

Cell Proliferation Induced by VEGF165and Neutralization by Human VEGF Antibody.

Recombinant Human VEGF165 (293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293). The ND50 is typically 10-60 ng/mL.
Detection of Rat VEGF by Western Blot

Detection of Rat VEGF by Western Blot

Protein levels of VEGF (A), KDR (VEGF receptor 2, B), Angiopoietin 1 (C) and Tie2 (D) from retinal endothelial cells cultured in normoxia or hypoxia only and cells in each condition treated with Compound 49b. Hypoxia increased protein levels of all proteins, which were significantly reduced after treatment with Compound 49b. Data is mean ± SEM. *P<0.05 vs. normoxia. #P<0.05 vs. hypoxia. N = 4. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27439004), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Primate VEGF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human VEGF165 (Catalog # 293-VE)

Neutralization

Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-60 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165.

Human/Primate VEGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate VEGF165 Biotinylated Antibody (Catalog # BAF293)
  • Standard: Recombinant Human VEGF 165 Protein (Catalog # 293-VE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 8 reviews rated 4.8 using MAB293 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

NP_001165097.1

Additional VEGF Products

Product Documents for Human/Primate VEGF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Primate VEGF Antibody

For research use only

Citations for Human/Primate VEGF Antibody

Customer Reviews for Human/Primate VEGF Antibody (8)

4.8 out of 5
8 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • Human/Primate VEGF Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 02/14/2022
    Human/Primate VEGF Antibody MAB293
  • Human/Primate VEGF Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Metastatic breast cancer
    Species: Human
    Verified Customer | Posted 08/09/2021
    Human/Primate VEGF Antibody MAB293
  • Human/Primate VEGF Antibody
    Name: Karina Kinghorn
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 07/16/2021
    IF of HUVEC cells with exogenous VEGF internalized (red) and colocalizing with VEGF receptor (green)
    Human/Primate VEGF Antibody MAB293
  • Human/Primate VEGF Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Serum-free Cell Culture Media and Breast cancer cells
    Species: Human
    Verified Customer | Posted 05/27/2019
  • Human/Primate VEGF Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 03/13/2019
    We used this VEGF-A neutralizing antibody (10 ng/mL) as part of an antibody cocktail containing several other antibodies to neutralize VEGF-A present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II CM (+/- VEGF-A Neutralizing ab Cocktail) was used to further look at Endothelial (HUVEC) Cell migration using Incucyte Chemotaxis Assay. Cancer Res; 78(5) March 1, 2018 (Fig 6F)
    Human/Primate VEGF Antibody MAB293
  • Human/Primate VEGF Antibody
    Name: Zoia Levashova
    Application: Meso Scale Discovery
    Sample Tested: Retina/choroid, Vitreous humor and Aqueous humor
    Species: Porcine
    Verified Customer | Posted 06/22/2018
    After biotinylation, used as a capture reagent in MSD assay to detect ocular VEGF level in a swine model of laser-induced choroidal neovascularization. A standard curve with pig VEGF from Bio-Rad (Cat# PPP030) is shown (2-8,000 pg/ml).
    Human/Primate VEGF Antibody MAB293
  • Human/Primate VEGF Antibody
    Name: Alisha Freeman
    Application: ELISA
    Sample Tested: CHO Chinese hamster ovary cell line
    Species: Hamster
    Verified Customer | Posted 11/04/2017
  • Human/Primate VEGF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 08/18/2017
    Human/Primate VEGF Antibody MAB293

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FAQs for Human/Primate VEGF Antibody

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  • Q: What are the differences between MAB293 and MAB293R?

    A: MAB293 is produced by culturing a hybridoma cell line, whereas MAB293R is produced via recombinant DNA technology, based on the known, proprietary sequence of MAB293.  The benefit of recombinant antibodies is the ability to offer a more consistent antibody, with an indefinite supply.  As far as research applications, both products will work the same.

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