hh Human VEGF165 Antibody (AF-293-NA): R&D Systems

Human VEGF165 Antibody

(34 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant canine VEGF is observed, and less then 20% cross-reactivity with recombinant mouse VEGF165 and recombinant rat VEGF164 is observed, and less than 5% cross-reactivity with recombinant zebrafish VEGF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165
    Ala27-Arg191
    Accession # NP_001165097.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    5-10 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The Neutralization Dose (ND50) is typically 0.02‑0.12 µg/mL in the presence of 10 ng/mL Recombinant Human VEGF165.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human VEGF by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, and human liver tissue. PVDF membrane was probed with 5-10 µg/mL of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VEGF at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
VEGF in HUVEC Cells. VEGF was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑293‑NA). The ND50 is typically 0.02-0.12 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF
VEGF is a soluble protein secreted by a wide variety of cell types. It binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (Flk-1). VEGF stimulates vascular endothelial cell proliferation and is a potent inducer of angiogenesis. Several VEGF isoforms occur resulting from alternative mRNA splicing.
  • Long Name:
    Vascular Endothelial Growth Factor
  • Entrez Gene IDs:
    7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
  • Alternate Names:
    MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression
    Authors: WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung
    Nat Commun, 2017;8(0):14399.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells
    Authors: S Yang, F Qi, C Tang, H Wang, H Qin, X Li, J Li, W Wang, C Zhao, H Gao
    Oncol Lett, 2017;13(2):898-904.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
    Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
    Oncotarget, 2016;7(47):77902-77915.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
    Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J
    Mol Ther, 2014;22(7):1243-53.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
    Authors: Orecchia A, Mettouchi A, Uva P, Simon G, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger K, Zambruno G, Failla C
    FASEB J, 2014;28(2):692-704.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Detection of VEGF-A(xxx)b isoforms in human tissues.
    Authors: Bates, David O, Mavrou, Athina, Qiu, Yan, Carter, James G, Hamdollah-Zadeh, Maryam, Barratt, Shaney, Gammons, Melissa, Millar, Ann B, Salmon, Andrew H, Oltean, Sebastia, Harper, Steven J
    PLoS ONE, 2013;8(7):e68399.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  7. Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.
    Authors: Ruffini F, Tentori L, Dorio A, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal P
    Oncol Rep, 2013;30(6):2887-96.
  8. Simple patterned nanofiber scaffolds and its enhanced performance in immunoassay.
    Authors: Wang, Jing, Kang, Qin-shu, Lv, Xiao-gua, Song, Jia, Zhan, Na, Dong, Wei-guo, Huang, Wei-hua
    PLoS ONE, 2013;8(12):e82888.
    Species: Human
    Sample Type: Recombinant Protein
    Application: micro-immunoassay development
  9. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  10. Chronic exposure of colorectal cancer cells to bevacizumab promotes compensatory pathways that mediate tumour cell migration.
    Authors: Fan F, Samuel S, Gaur P
    Br. J. Cancer, 2011;104(8):1270-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
    Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
    Angiogenesis, 2010;13(4):337-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. The hypoxia-inducible factor 1/NOR-1 axis regulates the survival response of endothelial cells to hypoxia.
    Authors: Martorell L, Gentile M, Rius J, Rodriguez C, Crespo J, Badimon L, Martinez-Gonzalez J
    Mol. Cell. Biol., 2009;29(21):5828-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo.
    Authors: Rennel ES, Varey AH, Churchill AJ, Wheatley ER, Stewart L, Mather S, Bates DO, Harper SJ
    Br. J. Cancer, 2009;101(7):1183-93.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Effect of chemotherapeutic stress on induction of vascular endothelial growth factor family members and receptors in human colorectal cancer cells.
    Authors: Fan F, Gray MJ, Dallas NA, Yang AD, Van Buren G, Camp ER, Ellis LM
    Mol. Cancer Ther., 2008;7(9):3064-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
    Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
    J. Biol. Chem., 2008;283(49):34250-9.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  16. Comparison of bevacizumab, ranibizumab, and pegaptanib in vitro: efficiency and possible additional pathways.
    Authors: Klettner A, Roider J
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4523-7.
    Species: Porcine
    Sample Type: Cell Lysates
    Application: WB
  17. Mammary alveolar development during lactation is inhibited by the endogenous antiangiogenic growth factor isoform, VEGF165b.
    Authors: Qiu Y, Bevan H, Weeraperuma S, Wratting D, Murphy D, Neal CR, Bates DO, Harper SJ
    FASEB J., 2007;22(4):1104-12.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  18. Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment.
    Authors: Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA
    Blood, 2007;109(1):122-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: ICC
  19. Bactericidal/permeability-increasing protein's signaling pathways and its retinal trophic and anti-angiogenic effects.
    Authors: Yamagata M, Rook SL, Sassa Y, Ma RC, Geraldes P, Goddard L, Clermont A, Gao B, Salti H, Gundel R, White M, Feener EP, Aiello LP, King GL
    FASEB J., 2006;20(12):2058-67.
    Species: Bovine
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  20. The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
    Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
    Clin. Sci., 2006;110(5):575-85.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  21. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
    Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
    Glia, 2006;53(8):845-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  22. Inhibition of cyclooxygenase-2 disrupts tumor vascular mural cell recruitment and survival signaling.
    Authors: Lee A, Frischer J, Serur A, Huang J, Bae JO, Kornfield ZN, Eljuga L, Shawber CJ, Feirt N, Mansukhani M, Stempak D, Baruchel S, Bender JG, Kandel JJ, Yamashiro DJ
    Cancer Res., 2006;66(8):4378-84.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  23. Age-associated aortic stenosis in apolipoprotein E-deficient mice.
    Authors: Tanaka K, Sata M, Fukuda D, Suematsu Y, Motomura N, Takamoto S, Hirata Y, Nagai R
    J. Am. Coll. Cardiol., 2005;46(1):134-41.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  24. The proline-rich homeodomain (PRH/HEX) protein is down-regulated in liver during infection with lymphocytic choriomeningitis virus.
    Authors: Djavani M, Topisirovic I, Zapata JC, Sadowska M, Yang Y, Rodas J, Lukashevich IS, Bogue CW, Pauza CD, Borden KL, Salvato MS
    J. Virol., 2005;79(4):2461-73.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  25. Human peripheral blood eosinophils induce angiogenesis.
    Authors: Puxeddu I, Alian A, Piliponsky AM, Ribatti D, Panet A, Levi-Schaffer F
    Int. J. Biochem. Cell Biol., 2005;37(3):628-36.
    Species: Chicken
    Sample Type: Whole Tissue
  26. Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath.
    Authors: Vijayan V, Shukla N, Johnson JL, Gadsdon P, Angelini GD, Smith FC, Baird R, Jeremy JY
    J. Vasc. Surg., 2004;40(5):1011-9.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  27. Alterations in angiogenic growth factors and neuronal nitric oxide synthase expression in chronic cavernosal ischemia.
    Authors: Wang T, Soker S, Atala A, Siroky MB, Azadzoi KM
    Int. J. Impot. Res., 2004;16(5):403-11.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC
  28. Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.
    Authors: Wright JL, Tai H, Churg A
    Am. J. Respir. Cell Mol. Biol., 2004;31(5):501-9.
    Species: Guinea Pig
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  29. Estrogen enhances angiogenesis through a pathway involving platelet-activating factor-mediated nuclear factor-kappaB activation.
    Authors: Seo KH, Lee HS, Jung B, Ko HM, Choi JH, Park SJ, Choi IH, Lee HK, Im SY
    Cancer Res., 2004;64(18):6482-8.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Neut
  30. Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
    Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
    Int. J. Cancer, 2003;104(5):603-10.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  31. Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
    Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
    Blood, 2003;102(2):638-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  32. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: Human
    Sample Type: BALF
    Application: ELISA Development
  33. VEGF signaling is required for the assembly but not the maintenance of embryonic blood vessels.
    Authors: Argraves WS, Larue AC, Fleming PA, Drake CJ
    Dev. Dyn., 2002;225(3):298-304.
    Species: Avian - Quail
    Sample Type: In Vivo
    Application: In vivo
  34. VEGF in the lung: a role for novel isoforms.
    Authors: Varet, Julia, Varet J, Douglas SK, Douglas, Samantha, Gilmartin, Laura, Gilmartin L, Medford, Andrew R, Bates, David O, Harper, Steven J, Millar, Ann B
    Am J Physiol Lung Cell Mol Physiol, 0;298(6):L768-74.
    Species: Human
    Sample Type: BALF
    Application: ELISA Development
Expand to show all 34 Citations
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Block/Neutralize.

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 Block/Neutralize Human Anonymous 11/23/2015
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Summary

ApplicationBlock/Neutralize
Sample TestedPrimary pumonary arterial endothelial cell lysate
SpeciesHuman

Other Experimental Details

Other Experimental DetailsNeutralization of recombinant VEGF-induced VEGFR2 phosphorylation.

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