Human VEGF165 Antibody

Catalog # Availability Size / Price Qty
AF-293-NA
AF-293-SP
Detection of Human VEGF by Western Blot.
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Product Details
Citations (39)
FAQs
Supplemental Products
Reviews (2)

Human VEGF165 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant canine VEGF is observed, and less then 20% cross-reactivity with recombinant mouse VEGF165 and recombinant rat VEGF164 is observed, and less than 5% cross-reactivity with recombinant zebrafish VEGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165
Ala27-Arg191
Accession # NP_001165097.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
5-10 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The Neutralization Dose (ND50) is typically 0.02‑0.12 µg/mL in the presence of 10 ng/mL Recombinant Human VEGF165.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human VEGF by Western Blot. View Larger

Detection of Human VEGF by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, and human liver tissue. PVDF membrane was probed with 5-10 µg/mL of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VEGF at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry VEGF in HUVEC Cells. View Larger

VEGF in HUVEC Cells. VEGF was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. View Larger

Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑293‑NA). The ND50 is typically 0.02-0.12 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

VEGF is a soluble protein secreted by a wide variety of cell types. It binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (Flk-1). VEGF stimulates vascular endothelial cell proliferation and is a potent inducer of angiogenesis. Several VEGF isoforms occur resulting from alternative mRNA splicing.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Product Datasheets

Citations for Human VEGF165 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages
    Authors: S Donzelli, E Milano, M Pruszko, A Sacconi, S Masciarell, I Iosue, E Melucci, E Gallo, I Terrenato, M Mottolese, M Zylicz, A Zylicz, F Fazi, G Blandino, G Fontemaggi
    Breast Cancer Res., 2018;20(1):59.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
    Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Applications: Affinity Assay
  3. CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells
    Authors: S Yang, F Qi, C Tang, H Wang, H Qin, X Li, J Li, W Wang, C Zhao, H Gao
    Oncol Lett, 2017;13(2):898-904.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression
    Authors: WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung
    Nat Commun, 2017;8(0):14399.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  5. A randomized, single-blind, Phase I trial (INVICTAN-1) assessing the bioequivalence and safety of BI 695502, a bevacizumab biosimilar candidate, in healthy subjects
    Authors: W Hettema, C Wynne, B Lang, M Altendorfe, N Czeloth, R Lohmann, S Athalye, D Schliephak
    Expert Opin Investig Drugs, 2017;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: ELISA detection
  6. Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
    Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
    PLoS ONE, 2017;12(9):e0184039.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  7. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
    Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
    Oncotarget, 2016;7(47):77902-77915.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  8. Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
    Authors: Orecchia A, Mettouchi A, Uva P, Simon G, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger K, Zambruno G, Failla C
    FASEB J, 2014;28(2):692-704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  9. Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
    Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J
    Mol Ther, 2014;22(7):1243-53.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  10. Detection of VEGF-A(xxx)b isoforms in human tissues.
    Authors: Bates, David O, Mavrou, Athina, Qiu, Yan, Carter, James G, Hamdollah-Zadeh, Maryam, Barratt, Shaney, Gammons, Melissa, Millar, Ann B, Salmon, Andrew H, Oltean, Sebastia, Harper, Steven J
    PLoS ONE, 2013;8(7):e68399.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  11. Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.
    Authors: Ruffini F, Tentori L, Dorio A, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal P
    Oncol Rep, 2013;30(6):2887-96.
  12. Simple patterned nanofiber scaffolds and its enhanced performance in immunoassay.
    Authors: Wang, Jing, Kang, Qin-shu, Lv, Xiao-gua, Song, Jia, Zhan, Na, Dong, Wei-guo, Huang, Wei-hua
    PLoS ONE, 2013;8(12):e82888.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: micro-immunoassay development
  13. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  14. Chronic exposure of colorectal cancer cells to bevacizumab promotes compensatory pathways that mediate tumour cell migration.
    Authors: Fan F, Samuel S, Gaur P
    Br. J. Cancer, 2011;104(8):1270-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  15. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
    Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
    Angiogenesis, 2010;13(4):337-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  16. The hypoxia-inducible factor 1/NOR-1 axis regulates the survival response of endothelial cells to hypoxia.
    Authors: Martorell L, Gentile M, Rius J, Rodriguez C, Crespo J, Badimon L, Martinez-Gonzalez J
    Mol. Cell. Biol., 2009;29(21):5828-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  17. VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo.
    Authors: Rennel ES, Varey AH, Churchill AJ, Wheatley ER, Stewart L, Mather S, Bates DO, Harper SJ
    Br. J. Cancer, 2009;101(7):1183-93.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  18. Effect of chemotherapeutic stress on induction of vascular endothelial growth factor family members and receptors in human colorectal cancer cells.
    Authors: Fan F, Gray MJ, Dallas NA, Yang AD, Van Buren G, Camp ER, Ellis LM
    Mol. Cancer Ther., 2008;7(9):3064-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  19. Comparison of bevacizumab, ranibizumab, and pegaptanib in vitro: efficiency and possible additional pathways.
    Authors: Klettner A, Roider J
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4523-7.
    Species: Porcine
    Sample Types: Cell Lysates
    Applications: WB
  20. Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
    Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
    J. Biol. Chem., 2008;283(49):34250-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  21. Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment.
    Authors: Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA
    Blood, 2007;109(1):122-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  22. Mammary alveolar development during lactation is inhibited by the endogenous antiangiogenic growth factor isoform, VEGF165b.
    Authors: Qiu Y, Bevan H, Weeraperuma S, Wratting D, Murphy D, Neal CR, Bates DO, Harper SJ
    FASEB J., 2007;22(4):1104-12.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  23. The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
    Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
    Clin. Sci., 2006;110(5):575-85.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  24. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
    Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
    Glia, 2006;53(8):845-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  25. Inhibition of cyclooxygenase-2 disrupts tumor vascular mural cell recruitment and survival signaling.
    Authors: Lee A, Frischer J, Serur A, Huang J, Bae JO, Kornfield ZN, Eljuga L, Shawber CJ, Feirt N, Mansukhani M, Stempak D, Baruchel S, Bender JG, Kandel JJ, Yamashiro DJ
    Cancer Res., 2006;66(8):4378-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Bactericidal/permeability-increasing protein's signaling pathways and its retinal trophic and anti-angiogenic effects.
    Authors: Yamagata M, Rook SL, Sassa Y, Ma RC, Geraldes P, Goddard L, Clermont A, Gao B, Salti H, Gundel R, White M, Feener EP, Aiello LP, King GL
    FASEB J., 2006;20(12):2058-67.
    Species: Bovine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  27. Human peripheral blood eosinophils induce angiogenesis.
    Authors: Puxeddu I, Alian A, Piliponsky AM, Ribatti D, Panet A, Levi-Schaffer F
    Int. J. Biochem. Cell Biol., 2005;37(3):628-36.
    Species: Chicken
    Sample Types: Whole Tissue
  28. The proline-rich homeodomain (PRH/HEX) protein is down-regulated in liver during infection with lymphocytic choriomeningitis virus.
    Authors: Djavani M, Topisirovic I, Zapata JC, Sadowska M, Yang Y, Rodas J, Lukashevich IS, Bogue CW, Pauza CD, Borden KL, Salvato MS
    J. Virol., 2005;79(4):2461-73.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  29. Age-associated aortic stenosis in apolipoprotein E-deficient mice.
    Authors: Tanaka K, Sata M, Fukuda D, Suematsu Y, Motomura N, Takamoto S, Hirata Y, Nagai R
    J. Am. Coll. Cardiol., 2005;46(1):134-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Estrogen enhances angiogenesis through a pathway involving platelet-activating factor-mediated nuclear factor-kappaB activation.
    Authors: Seo KH, Lee HS, Jung B, Ko HM, Choi JH, Park SJ, Choi IH, Lee HK, Im SY
    Cancer Res., 2004;64(18):6482-8.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Neut
  31. Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath.
    Authors: Vijayan V, Shukla N, Johnson JL, Gadsdon P, Angelini GD, Smith FC, Baird R, Jeremy JY
    J. Vasc. Surg., 2004;40(5):1011-9.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  32. Alterations in angiogenic growth factors and neuronal nitric oxide synthase expression in chronic cavernosal ischemia.
    Authors: Wang T, Soker S, Atala A, Siroky MB, Azadzoi KM
    Int. J. Impot. Res., 2004;16(5):403-11.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  33. Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.
    Authors: Wright JL, Tai H, Churg A
    Am. J. Respir. Cell Mol. Biol., 2004;31(5):501-9.
    Species: Guinea Pig
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  34. Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
    Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
    Int. J. Cancer, 2003;104(5):603-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  35. Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
    Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
    Blood, 2003;102(2):638-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  36. VEGF signaling is required for the assembly but not the maintenance of embryonic blood vessels.
    Authors: Argraves WS, Larue AC, Fleming PA, Drake CJ
    Dev. Dyn., 2002;225(3):298-304.
    Species: Avian - Quail
    Sample Types: In Vivo
    Applications: In vivo
  37. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  38. VEGF in the lung: a role for novel isoforms.
    Authors: Varet, Julia, Varet J, Douglas SK, Douglas, Samantha, Gilmartin, Laura, Gilmartin L, Medford, Andrew R, Bates, David O, Harper, Steven J, Millar, Ann B
    Am J Physiol Lung Cell Mol Physiol, 0;298(6):L768-74.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  39. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
    Authors: Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
    Stem Cells, 0;34(4):1112-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut

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Reviews for Human VEGF165 Antibody

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Human VEGF165 Antibody
By Anonymous on 06/22/2018
Application: MSD &#40;Meso Scale Discovery&#41; assay Sample Tested: Vitreous humor,Aqueous humor,Retina/choroid Species: Porcine

After labeling with Sulfo-Tag, used as a detection reagent in MSD assay to detect ocular VEGF level in a swine model of laser-induced choroidal neovascularization. A standard curve with pig VEGF from Bio-Rad (Cat# PPP030) is shown (2-8,000 pg/ml).


Human VEGF165 Antibody
By Anonymous on 11/23/2015
Application: B/N Sample Tested: Primary pumonary arterial endothelial cell lysate Species: Human

Neutralization of recombinant VEGF-induced VEGFR2 phosphorylation.