TGF-beta 2 Antibody Summary
Applications
under non-reducing conditions only
Human TGF-beta 2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TGF‑ beta 2 in Human Prostate Cancer. TGF-beta 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-TGF-beta 2 Monoclonal Antibody (Catalog # MAB612) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of TGF-beta 2 by Western Blot Effects of transforming growth factor (TGF)-beta 2 silencing on metastatic phenotypes of sonic-hedgehog medulloblastoma cells with high LOXL1-AS1 expression. A RT-qPCR analysis of TGF-beta 2 expression in Daoy-LOXL1-AS1 and Daoy-MYCN cells. B RT-qPCR analysis of four stemness markers in Daoy-LOXL1-AS1 and Daoy-MYCN cells relative to the parental group. C Western blot analysis of four stemness markers in Daoy-LOXL1-AS1 and Daoy-MYCN cells. Quantification values below each band are relative to the parental group. D, G Sphere-formation assay of (D) Daoy-LOXL1-AS1 and (G) Daoy-MYCN cells, including microscopic images (left images), and the number and sizes of spheres of ≥150 μm in diameter (right bar chart). Scale bars, 500 μm in 4× and 100 μm in 20× magnification. E, H Wound-healing assay of (E) Daoy-LOXL1-AS1 and (H) Daoy-MYCN cells. F, I Transwell migration and invasion assays of (F) Daoy-LOXL1-AS1 and (I) Daoy-MYCN cells including quantification of cell migration (left bar chart) and invasion (right bar chart). Quantitative data are presented as mean ± SD of replicates from representative of three independent experiments. NC, negative control; ns, non-significant. * p < 0.05, ** p < 0.01, *** p < 0.001 Image collected and cropped by CiteAb from the following open publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-024-03057-0), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 2
The active form of TGF-beta 2 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 2 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.
Product Datasheets
Citations for TGF-beta 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
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TGF-beta isoforms in cancer: Immunohistochemical expression and Smad-pathway-activity-analysis in thirteen major tumor types with a critical appraisal of antibody specificity and immunohistochemistry assay validity
Authors: Markus J. Riemenschneider, Maria Hirblinger, Arabel Vollmann-Zwerenz, Peter Hau, Martin A. Proescholdt, Frank Jaschinski et al.
Oncotarget
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Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype
Authors: N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh
Anticancer Res., 2018-04-01;38(4):2007-2014.
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Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells
Authors: Oana Tudoran, Olga Soritau, Ovidiu Balacescu, Loredana Balacescu, Cornelia Braicu, Meda Rus et al.
Journal of Cellular and Molecular Medicine
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Insights into homeobox B9: a propeller for metastasis in dormant prostate cancer progenitor cells
Authors: Y Sui, W Hu, W Zhang, D Li, H Zhu, Q You, R Zhu, Q Yi, T Tang, L Gao, S Zhu, T Yang
British Journal of Cancer, 2021-07-10;0(0):.
Species: Xenograft
Sample Types: Whole Cells
Applications: Western Blot -
Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis.
Authors: Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J
Nat Commun, 2016-03-18;7(0):11012.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins.
Authors: Kawahara S, Hata Y, Kita T, Arita R, Miura M, Nakao S, Mochizuki Y, Enaida H, Kagimoto T, Goto Y, Hafezi-Moghadam A, Ishibashi T
Diabetes, 2008-07-03;57(10):2784-93.
Species: Human
Sample Types: Vitreous Humor
Applications: Neutralization -
Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads.
Authors: Wen FQ, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, Ertl R, Terasaki Y, Manouilova L, Rennard SI
Am. J. Respir. Cell Mol. Biol., 2004-01-12;30(6):816-22.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56.
Species: Human
Sample Types: Cell Culture Supernates
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TGF beta 1 and TGF beta 2 proteins in corneas with and without stromal fibrosis: Delayed regeneration of apical epithelial growth factor barrier and the epithelial basement membrane in corneas with stromal fibrosis
Authors: Carlos de Oliveira R, Tye G, Sampaio L et al.
Experimental Eye Research
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Blood pressure homeostasis is maintained by a P311–TGF-beta axis
Authors: Kameswara Rao Badri, Ming Yue, Oscar A. Carretero, Sree Latha Aramgam, Jun Cao, Stephen Sharkady et al.
Journal of Clinical Investigation
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I used this antibody as a capture antibody to generate an in-house ELISA for TGF beta. I coated the plate overnight with the antibody at 2ug/mL in PBS 100uL/well
