Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 8607
View all TGF-beta 2 Primary Antibodies »

Product Specifications

Immunogen

Porcine TGF-beta 1.2

Specificity

Detects TGF-beta 2 from multiple species in ELISAs and Western blots. In ELISAs, no cross-reactivity or interfereence with recombinant human (rh) TGF-alpha, recombinant porcineTGF-beta 1, rhTGF-beta 1, recombinant canine TGF-beta 3, recombinant amphibian TGF-beta 5, rhTGF-beta RII, or rhTGF-beta RIII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TGF‑ beta 2 Antibody

TGF-beta 2 antibody in Human Prostate Cancer by Immunohistochemistry (IHC-P).

TGF‑ beta 2 in Human Prostate Cancer.

TGF-beta 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-TGF-beta 2 Monoclonal Antibody (Catalog # MAB612) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of TGF-beta 2 by Western Blot

Detection of TGF-beta 2 by Western Blot

Effects of transforming growth factor (TGF)-beta 2 silencing on metastatic phenotypes of sonic-hedgehog medulloblastoma cells with high LOXL1-AS1 expression. A RT-qPCR analysis of TGF-beta 2 expression in Daoy-LOXL1-AS1 and Daoy-MYCN cells. B RT-qPCR analysis of four stemness markers in Daoy-LOXL1-AS1 and Daoy-MYCN cells relative to the parental group. C Western blot analysis of four stemness markers in Daoy-LOXL1-AS1 and Daoy-MYCN cells. Quantification values below each band are relative to the parental group. D, G Sphere-formation assay of (D) Daoy-LOXL1-AS1 and (G) Daoy-MYCN cells, including microscopic images (left images), and the number and sizes of spheres of ≥150 μm in diameter (right bar chart). Scale bars, 500 μm in 4× and 100 μm in 20× magnification. E, H Wound-healing assay of (E) Daoy-LOXL1-AS1 and (H) Daoy-MYCN cells. F, I Transwell migration and invasion assays of (F) Daoy-LOXL1-AS1 and (I) Daoy-MYCN cells including quantification of cell migration (left bar chart) and invasion (right bar chart). Quantitative data are presented as mean ± SD of replicates from representative of three independent experiments. NC, negative control; ns, non-significant. * p < 0.05, ** p < 0.01, *** p < 0.001 Image collected and cropped by CiteAb from the following open publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-024-03057-0), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for TGF‑ beta 2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample:  Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta 2 (Catalog # 302-B2)
under non-reducing conditions only

Human TGF-beta 2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: TGF‑ beta 2 Biotinylated Antibody (Catalog # BAF302)
  • Standard: Recombinant Human TGF-beta 2 Protein (Catalog # 302-B2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using MAB612 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 2

The active form of TGF-beta 2 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 2 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.

Long Name

Transforming Growth Factor beta 2

Alternate Names

TGFB2, TGFbeta 2

Entrez Gene IDs

7042 (Human); 21808 (Mouse); 397084 (Porcine)

Gene Symbol

TGFB2

Additional TGF-beta 2 Products

Product Documents for TGF‑ beta 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for TGF‑ beta 2 Antibody

For research use only

Citations for TGF‑ beta 2 Antibody

Customer Reviews for TGF‑ beta 2 Antibody (3)

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3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • TGF-beta 2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 11/07/2021
    TGF‑ beta 2 Antibody MAB612
  • TGF-beta 2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 03/28/2019
    I used this antibody as a capture antibody to generate an in-house ELISA for TGF beta. I coated the plate overnight with the antibody at 2ug/mL in PBS 100uL/well
    TGF‑ beta 2 Antibody MAB612
  • TGF-beta 2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 Cell Lysate
    Species: Human
    Verified Customer | Posted 04/25/2017
    MDA-MB-231 cell extract probed with Mouse anti-Human TGF-beta 2 antibody (#MAB612) at 1 ug/mL, followed by Goat anti-Mouse secondary antibody at 1:5,000 dilution. PVDF membrane blocked with 5% BSA-PBS.
    TGF‑ beta 2 Antibody MAB612

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Protocols

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