LI-COR Data Examples for Human Kidney Biomarker Array Kit

Data Examples

Figure 1. The Human Kidney Biomarker Array detects multiple analytes in cell culture supernates.Cells were either untreated or treated as indicated below. Each array was incubated with 500 µL of cell culture supernate. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
  1. 786-O human renal cell adenocarcinoma cells, untreated
  2. HK-2 human proximal tubule epithelial cells, untreated
  3. Peripheral blood mononuclear cells were either untreated or treated with 10 µg/mL of PHA for 24 h.
  Integrated Intensity
HK-2 Kidney Cortex Urine (ARI) Urine (RCC)
1
ANPEP
745 26075 7701 2089
2
Angiotensinogen
1039 1884 11223 3316
3
beta 2-Microglobulin
14935 35032 46606 68270
4
Cyr61
13191 547 2228 1245
5
Cystatin C
3172 5316 58281 8194
6
DPPIV
7107 49503 5279 1310
7
EGF
3058 1492 45762 8163
8
EGF R
17183 2045 7433 1421
9
FABP1
792 1091 40978 1665
10
GRO alpha
749 573 18450 1176
11
TIM-1
18960 1071 3158 1202
12
Lipocalin-2
2453 8488 63866 20930
13
MMP-9
782 764 50390 2562
14
RAGE
740 617 8905 2521
15
RBP4
1153 12745 51945 18536
16
Resistin
1263 1784 52208 4337
17
Serpin A3
710 3450 7649 17241
18
TNF RI
2526 1182 66429 13216
19
TFF3
785 853 55535 61720
20
uPA
9140 637 60493 3947
21
VCAM-1
964 3084 62586 10968
22
VEGF
1243 728 39223 1833
Figure 2. The Human Kidney Biomarker Array detects multiple analytes in cell culture lysates, tissue lysates, and urine. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
  1. HK-2 human proximal tubule epithelial cells, untreated; 150 µg of cell culture lysate per array.
  2. Kidney cortex; 20 µg of tissue lysate per array.
  3. Urine from a patient with Acute Renal Injury (ARI); 500 µL per array.
  4. Urine from a patient with Renal Cell Carcinoma (RCC);500 µL per array.

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