Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are diluted and incubated with the Human Ubiquitin Array overnight. The array is washed to remove unbound proteins, followed by incubation with a biotinylated detection antibody. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the level of ubiquitinylation of the bound protein. Chemiluminescence is detected in the same manner as a Western blot.
4 Array Membranes
Detection Antibody Cocktail
Chemiluminescent Detection Reagents
Transparency Overlay Template
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8° C.
Simultaneously detect the Ubiquitination of these proteins in a single sample.
F-box protein 15/FBXO15
I kappa B alpha
I kappa B eplison
PDGF R alpha
PDGF R beta
Assays and antibodies for Analytes represented in the Human Ubiquitin Array Kit
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