The Proteome Profiler Mouse Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Store the unopened kit at 2°C to 8°C. Do not use past kit expiration date.
|Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample.|
|DPPIV/CD26||Leptin||Coagulation Factor III|
|EGF||MMP-3 (pro and mature)||VEGF|
|Endostatin/Collagen XVIII||MMP-9 (pro and active)|
|Quantkine® ELISA kit||DuoSet® ELISA Development Reagents|
|MMP-3 (pro and mature)||MMP300|
|MMP-9 (pro and active)||MMPT90|
|Coagulation Factor III|
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Multiple Proteins in TNF-alpha-treated Balb/3T3 Cells were Assessed using the Mouse Angiogenesis Array. A. The Proteome Profiler Mouse Angiogenesis Array Kit (Catalog # ARY015) was used to simultaneously assess the relative levels of 53 mouse angiogenesis-related proteins in cell lysates from Balb/3T3 mouse embryonic fibroblast cells that had been left untreated or treated with recombinant mouse TNF-alpha (Catalog # 410-MT) for 24 hours. B. Histogram profiles for select proteins were generated by quantifying the mean spot pixel density from the arrays using image software analysis. Green bars represent protein levels in lysates from untreated cells, purple bars represent protein levels in lysates from TNF-alpha-treated cells.
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