Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates or serum.

Key Benefits

  • Detects 53 mouse angiogenesis-related proteins simultaneously
  • Requires no specialized equipment
 

 

Principle of the Array

The Proteome Profiler Mouse Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.

Why Use an Antibody Array to Detect Angiogenesis-Related Proteins?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2°C to 8°C. Do not use past kit expiration date.

 

Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample.
ADAMTS1 Endothelin-1 NOV/CCN3/IGFBP-9
Amphiregulin FGF acidic Osteopontin
Angiogenin FGF basic PD-ECGF
Angiopoietin-1 FGF-7/KGF PDGF-AA
Angiopoietin-3 Fractalkine PDGF-AB/BB
CCL2/JE/MCP-1 GM-CSF Pentraxin-3
CCL3/MIP-1 alpha HB-EGF PlGF-2
CXCL1/KC HGF Prolactin
CXCL10/IP-10/CRG-2 IGFBP-1 Proliferin
CXCL12/SDF-1 IGFBP-2 Serpin E1/PAI-1
CXCL16 IGFBP-3 Serpin F1/PEDF
CXCL4/PF4 IL-1 alpha Thrombospondin-2
Cyr61/CNN1/IGFBP-10 IL-1 beta TIMP-1
DLL4 IL-10 TIMP-4
DPPIV/CD26 Leptin Coagulation Factor III
EGF MMP-3 (pro and mature) VEGF
Endoglin/CD105 MMP-8 (pro) VEGF-B
Endostatin/Collagen XVIII MMP-9 (pro and active)  

 

Assays for analytes represented in the Mouse Angiogenesis Antibody Array Kit

 

  Quantkine® ELISA kit DuoSet® ELISA Development Reagents
ADAMTS1    
Amphiregulin   DY989
Angiogenin    
Angiopoietin-1    
Angiopoietin-3 MANL30 DY738
CCL2/JE/MCP-1 MJE00 DY479
CCL3/MIP-1 alpha MMA00 DY450
CXCL1/KC MKC00B DY453
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL12/SDF-1 MCX120 DY460
CXCL16   DY503
CXCL4/PF4 MCX400 DY595
Cyr61/CNN1/IGFBP-10    
DLL4   DY1389
DPPIV/CD26   DY954
EGF MEG00 DY2028
Endoglin/CD105 MNDG00 DY1320
Endostatin/Collagen XVIII    
Endothelin-1 DET100  
FGF acidic    
FGF basic    
FGF-7/KGF    
Fractalkine MCX310 DY472
GM-CSF MGM00 DY415
HB-EGF    
HGF MHG00 DY2207
IGFBP-1    
IGFBP-2   DY797
IGFBP-3 MGB300 DY775
IL-1 alpha MLA00 DY400
IL-1 beta MLB00C DY401
IL-10 M1000B DY417
Leptin MOB00 DY498
MMP-3 (pro and mature) MMP300  
MMP-8 (pro)    
MMP-9 (pro and active) MMPT90  
NOV/CCN3/IGFBP-9   DY1976
Osteopontin MOST00 DY441
PD-ECGF    
PDGF-AA DAA00B  
PDGF-AB/BB MHD00/MBB00  
Pentraxin-3 MPTX30 DY2166
PlGF-2 MP200 DY465
Prolactin   DY1445
Proliferin    
Serpin E1/PAI-1    
Serpin F1/PEDF    
Thrombospondin-2    
TIMP-1 MTM100 DY980
TIMP-4    
Coagulation Factor III    
VEGF MMV00 DY493
VEGF-B   DY469

 

Data Examples

 

Multiple Proteins in TNF-alpha-treated Balb/3T3 Cells were Assessed using the Mouse Angiogenesis Array
View Larger Image

Multiple Proteins in TNF-alpha-treated Balb/3T3 Cells were Assessed using the Mouse Angiogenesis Array. A. The Proteome Profiler Mouse Angiogenesis Array Kit (Catalog # ARY015) was used to simultaneously assess the relative levels of 53 mouse angiogenesis-related proteins in cell lysates from Balb/3T3 mouse embryonic fibroblast cells that had been left untreated or treated with recombinant mouse TNF-alpha (Catalog # 410-MT) for 24 hours. B. Histogram profiles for select proteins were generated by quantifying the mean spot pixel density from the arrays using image software analysis. Green bars represent protein levels in lysates from untreated cells, purple bars represent protein levels in lysates from TNF-alpha-treated cells.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiogenesis Assay Kits
Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
  • Alternate Names:
    Angiogenesis Assay Kits
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
    Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
    Autophagy, 2017;0(0):0. 2017
  2. Downregulation of adaptor protein MyD88 compromises the angiogenic potential of B16 murine melanoma
    Authors: LD Trucco, E Roselli, P Araya, NG Nuñez, HA Mena, JL Bocco, S Negrotto, M Maccioni
    PLoS ONE, 2017;12(6):e0179897. 2017
  3. Mesenchymal stem cell carriers enhance antitumor efficacy of oncolytic adenoviruses in an immunocompetent mouse model
    Authors: E Rincón, T Cejalvo, D Kanojia, A Alfranca, MÁ Rodríguez-, RAG Hoyos, Y Han, L Zhang, R Alemany, MS Lesniak, J García-Cas
    Oncotarget, 2017;0(0):. 2017
  4. Mdivi-1 induced acute changes in the angiogenic profile after ischemia-reperfusion injury in female mice
    Authors: S Veeranki, SC Tyagi
    Physiol Rep, 2017;5(11):. 2017
  5. Nuclear FAK and Runx1 cooperate to regulate IGFBP3, cell cycle progression and tumor growth
    Authors: M Canel, A Byron, AH Sims, J Cartier, H Patel, MC Frame, VG Brunton, B Serrels, A Serrels
    Cancer Res., 2017;0(0):. 2017
  6. Integrin ?3/Akt signaling contributes to platelet-induced hemangioendothelioma growth
    Authors: R Gu, X Sun, Y Chi, Q Zhou, H Xiang, DB Bosco, X Lai, C Qin, KF So, Y Ren, XM Chen
    Sci Rep, 2017;7(1):6455. 2017
  7. Regulatory T cells promote myelin regeneration in the central nervous system
    Authors: Y Dombrowski, T O'Hagan, M Dittmer, R Penalva, SR Mayoral, P Bankhead, S Fleville, G Eleftheria, C Zhao, M Naughton, R Hassan, J Moffat, J Falconer, A Boyd, P Hamilton, IV Allen, A Kissenpfen, PN Moynagh, E Evergren, B Perbal, AC Williams, RJ Ingram, JR Chan, RJ Franklin, DC Fitzgerald
    Nat. Neurosci, 2017;0(0):. 2017
  8. Inhibition of PHD3 by salidroside promotes neovascularization through cell-cell communications mediated by muscle-secreted angiogenic factors
    Authors: J Zhang, V Kasim, YD Xie, C Huang, J Sisjayawan, A Dwi Ariyan, XS Yan, XY Wu, CP Liu, L Yang, M Miyagishi, SR Wu
    Sci Rep, 2017;7(0):43935. 2017
  9. Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma
    PLoS ONE, 2016;11(5):e0156151. 2016
  10. Granulocytic myeloid-derived suppressor cells promote angiogenesis in the context of multiple myeloma
    Oncotarget, 2016;7(25):37931-37943. 2016
  11. C-reactive protein can upregulate VEGF expression to promote ADSC-induced angiogenesis by activating HIF-1? via CD64/PI3k/Akt and MAPK/ERK signaling pathways
    Stem Cell Res Ther, 2016;7(1):114. 2016
  12. FAK regulates platelet extravasation and tumor growth after antiangiogenic therapy withdrawal
    Authors: M Haemmerle, J Bottsford-, S Pradeep, ML Taylor, HJ Choi, JM Hansen, HJ Dalton, RL Stone, MS Cho, AM Nick, AS Nagaraja, T Gutschner, KM Gharpure, LS Mangala, R Rupaimoole, HD Han, B Zand, GN Armaiz-Pen, SY Wu, CV Pecot, AR Burns, G Lopez-Bere
    J Clin Invest, 2016;0(0):. 2016
  13. Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells
    Authors: A Spiegel, MW Brooks, S Houshyar, F Reinhardt, M Ardolino, E Fessler, MB Chen, JA Krall, J DeCock, IK Zervantona, A Iannello, Y Iwamoto, V Cortez-Ret, RD Kamm, MJ Pittet, DH Raulet, RA Weinberg
    Cancer Discov, 2016;0(0):. 2016
  14. F-box protein FBXW7 inhibits cancer metastasis in a non-cell-autonomous manner.
    Authors: Yumimoto, Kanae, Akiyoshi, Sayuri, Ueo, Hiroki, Sagara, Yasuaki, Onoyama, Ichiro, Ueo, Hiroaki, Ohno, Shinji, Mori, Masaki, Mimori, Koshi, Nakayama, Keiichi
    J Clin Invest, 2015;125(2):621-35. 2015
  15. Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.
    Authors: Crouch E, Liu C, Silva-Vargas V, Doetsch F
    J Neurosci, 2015;35(11):4528-39. 2015
  16. Monocyte Chemoattractant Protein-Induced Protein 1 (MCPIP1) Enhances Angiogenic and Cardiomyogenic Potential of Murine Bone Marrow-Derived Mesenchymal Stem Cells.
    Authors: Labedz-Maslowska A, Lipert B, Berdecka D, Kedracka-Krok S, Jankowska U, Kamycka E, Sekula M, Madeja Z, Dawn B, Jura J, Zuba-Surma E
    PLoS ONE, 2015;10(7):e0133746. 2015
  17. Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
    Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M
    Stem Cell Reports, 2015;4(3):419-30. 2015
  18. Lysophosphatidic acid alters the expression profiles of angiogenic factors, cytokines, and chemokines in mouse liver sinusoidal endothelial cells.
    Authors: Chou C, Lai S, Ho C, Lin W, Chen C, Lee P, Peng F, Kuo S, Wu S, Lai H
    PLoS ONE, 2015;10(3):e0122060. 2015
  19. Lysyl oxidase-like 2 (LOXL2) and E47 EMT factor: novel partners in E-cadherin repression and early metastasis colonization.
    Authors: Canesin G, Cuevas E, Santos V, Lopez-Menendez C, Moreno-Bueno G, Huang Y, Csiszar K, Portillo F, Peinado H, Lyden D, Cano A
    Oncogene, 2015;34(8):951-64. 2015
  20. Application of VEGFA and FGF-9 enhances angiogenesis, osteogenesis and bone remodeling in type 2 diabetic long bone regeneration.
    Authors: Wallner C, Schira J, Wagner J, Schulte M, Fischer S, Hirsch T, Richter W, Abraham S, Kneser U, Lehnhardt M, Behr B
    PLoS ONE, 2015;10(3):e0118823. 2015
  21. Pathophysiology of lung injury induced by common bile duct ligation in mice.
    Authors: Shikata F, Sakaue T, Nakashiro K, Okazaki M, Kurata M, Okamura T, Okura M, Ryugo M, Nakamura Y, Yasugi T, Higashiyama S, Izutani H
    PLoS ONE, 2014;9(4):e94550. 2014
  22. TLR9 agonist regulates angiogenesis and inhibits corneal neovascularization.
    Authors: Wu J, Cui H, Dick A, Liu L
    Am J Pathol, 2014;184(6):1900-10. 2014
  23. c-Met inhibition in a HOXA9/Meis1 model of CN-AML.
    Authors: Mulgrew N, Kettyle L, Ramsey J, Cull S, Smyth L, Mervyn D, Bijl J, Thompson A
    Dev Dyn, 2014;243(1):172-81. 2014
  24. Platelet secretion and hemostasis require syntaxin-binding protein STXBP5.
    Authors: Ye S, Huang Y, Joshi S, Zhang J, Yang F, Zhang G, Smyth S, Li Z, Takai Y, Whiteheart S
    J Clin Invest, 2014;124(10):4517-28. 2014
  25. Capillary force seeding of hydrogels for adipose-derived stem cell delivery in wounds.
    Authors: Garg R, Rennert R, Duscher D, Sorkin M, Kosaraju R, Auerbach L, Lennon J, Chung M, Paik K, Nimpf J, Rajadas J, Longaker M, Gurtner G
    Stem Cells Transl Med, 2014;3(9):1079-89. 2014
  26. ADAMTS4 and its proteolytic fragments differentially affect melanoma growth and angiogenesis in mice.
    Authors: Rao N, Ke Z, Liu H, Ho C, Kumar S, Xiang W, Zhu Y, Ge R
    Int J Cancer, 2013;133(2):294-306. 2013
  27. Exosomes from retinal astrocytes contain antiangiogenic components that inhibit laser-induced choroidal neovascularization.
    Authors: Hajrasouliha, Amir Rez, Jiang, Guomin, Lu, Qingxian, Lu, Huayi, Kaplan, Henry J, Zhang, Huang-Ge, Shao, Hui
    J Biol Chem, 2013;288(39):28058-67. 2013
  28. Vascular normalization by loss of Siah2 results in increased chemotherapeutic efficacy.
    Authors: Wong CS, Sceneay J, House CM, Halse HM, Liu MC, George J, Hunnam TC, Parker BS, Haviv I, Ronai Z, Cullinane C, Bowtell DD, Moller A
    Cancer Res., 2012;72(7):1694-704. 2012
  29. Differentiation state determines neural effects on microvascular endothelial cells.
    Authors: Muffley L, Pan S, Smith A, Ga M, Hocking A, Gibran N
    Exp Cell Res, 2012;318(16):2085-93. 2012
  30. The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
    Authors: Tran K, Risingsong R, Royce D
    Cancer Prev Res (Phila), 2012;5(5):726-34. 2012
  31. ADAMTS5 functions as an anti-angiogenic and anti-tumorigenic protein independent of its proteoglycanase activity.
    Authors: Kumar S, Sharghi-Namini S, Rao N, Ge R
    Am J Pathol, 2012;181(3):1056-68. 2012
  32. Antimycotic ciclopirox olamine in the diabetic environment promotes angiogenesis and enhances wound healing.
    Authors: Ko SH, Nauta A, Morrison SD, Zhou H, Zimmermann A, Gurtner GC, Ding S, Longaker MT
    PLoS ONE, 2011;6(11):e27844. 2011
Expand to show all 32 Citations
Tocris Peptides
Description Application Cat# Citations Images  

Leupeptin hemisulfate

1167 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Pepstatin A

1190 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Proteome Profiler Mouse Angiogenesis Array Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

R&D Systems Guarantee

Customers Who Viewed
This Item Also Viewed

Feedback

Customer Information