Proteome Profiler Mouse Angiogenesis Array Kit
Proteome Profiler Mouse Angiogenesis Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates or serum.
- Detects 53 mouse angiogenesis-related proteins simultaneously
- Requires no specialized equipment
Principle of the Array
The Proteome Profiler Mouse Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.
Why Use an Antibody Array to Detect Angiogenesis-Related Proteins?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2°C to 8°C. Do not use past kit expiration date.
|Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample.|
|DPPIV/CD26||Leptin||Coagulation Factor III|
|EGF||MMP-3 (pro and mature)||VEGF|
|Endostatin/Collagen XVIII||MMP-9 (pro and active)|
Assays for analytes represented in the Mouse Angiogenesis Antibody Array Kit
|Quantkine® ELISA kit||DuoSet® ELISA Development Reagents|
|MMP-3 (pro and mature)||MMP300|
|MMP-9 (pro and active)||MMPT90|
|Coagulation Factor III|
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Multiple Proteins in TNF-alpha-treated Balb/3T3 Cells were Assessed using the Mouse Angiogenesis Array. A. The Proteome Profiler Mouse Angiogenesis Array Kit (Catalog # ARY015) was used to simultaneously assess the relative levels of 53 mouse angiogenesis-related proteins in cell lysates from Balb/3T3 mouse embryonic fibroblast cells that had been left untreated or treated with recombinant mouse TNF-alpha (Catalog # 410-MT) for 24 hours. B. Histogram profiles for select proteins were generated by quantifying the mean spot pixel density from the arrays using image software analysis. Green bars represent protein levels in lysates from untreated cells, purple bars represent protein levels in lysates from TNF-alpha-treated cells.
Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
Background: Angiogenesis Assay KitsAngiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
Citations for Proteome Profiler Mouse Angiogenesis Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Targeting AU-rich element-mediated mRNA decay with a truncated active form of the zinc-finger protein TIS11b/BRF1 impairs major hallmarks of mammary tumorigenesis
Authors: F Rataj, S Planel, J Denis, C Roelants, O Filhol, L Guyon, JJ Feige, N Cherradi
Oncogene, 2019;0(0):. 2019
The TRPC5 channel regulates angiogenesis and promotes recovery from ischemic injury in mice
Authors: Y Zhu, M Gao, T Zhou, M Xie, A Mao, L Feng, X Yao, WT Wong, X Ma
J. Biol. Chem., 2019;294(1):28-37. 2019
Pituitary adenylate cyclase-activating polypeptide ameliorates vascular dysfunction induced by hyperglycaemia
Authors: M Solymar, I Ivic, M Balasko, BD Fulop, G Toth, A Tamas, G Reman, A Koller, D Reglodi
Diab Vasc Dis Res, 2018;0(0):1479164118757. 2018
Mousetap, a Novel Technique to Collect Uncontaminated Vitreous or Aqueous and Expand Usefulness of Mouse Models
Authors: SD Fortmann, VE Lorenc, J Shen, SF Hackett, PA Campochiar
Sci Rep, 2018;8(1):6371. 2018
Endothelial Progenitor Cell Secretome and Oligovascular Repair in a Mouse Model of Prolonged Cerebral Hypoperfusion
Authors: T Maki, A Morancho, P Martinez-S, K Hayakawa, H Takase, AC Liang, M Gabriel-Sa, E Medina-Gut, K Washida, J Montaner, J Lok, EH Lo, K Arai, A Rosell
Stroke, 2018;0(0):. 2018
Adipocyte-secreted BMP8b mediates adrenergic-induced remodeling of the neuro-vascular network in adipose tissue
Authors: V Pellegrine, VJ Peirce, L Howard, S Virtue, D Türei, M Senzacqua, A Frontini, JW Dalley, AR Horton, G Bidault, I Severi, A Whittle, K Rahmouni, J Saez-Rodri, S Cinti, AM Davies, A Vidal-Puig
Nat Commun, 2018;9(1):4974. 2018
A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal
Authors: M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos
Cell Rep, 2018;25(13):3706-3720.e8. 2018
Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis
Authors: B Flood, J Manils, C Nulty, E Flis, S Kenealy, G Barber, J Fay, KHG Mills, EW Kay, EM Creagh
Oncogene, 2018;0(0):. 2018
A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
PLoS ONE, 2018;13(12):e0208579. 2018
Zika virus impairs the development of blood vessels in a mouse model of congenital infection
Authors: PP Garcez, HB Stolp, S Sravanam, RR Christoff, JCCG Ferreira, AA Dias, P Pezzuto, LM Higa, J Barbeito-A, RO Ferreira, CBV Andrade, M Siqueira, TMP Santos, J Drumond, A Hoerder-Su, CVF de Lima, F Tovar-Moll, RT Lopes, L Fragel-Mad, R Lent, TM Ortiga-Car, J Stipursky, M Bellio, A Tanuri, Z Molnár
Sci Rep, 2018;8(1):12774. 2018
Multipotent Adult Progenitor Cells Support Lymphatic Regeneration at Multiple Anatomical Levels during Wound Healing and Lymphedema
Authors: M Beerens, XL Aranguren, B Hendrickx, W Dheedene, T Dresselaer, U Himmelreic, C Verfaillie, A Luttun
Sci Rep, 2018;8(1):3852. 2018
Decellularized Diaphragmatic Muscle Drives a Constructive Angiogenic Response In Vivo
Authors: ME Alvarèz Fa, M Piccoli, C Franzin, A Sgrò, A Dedja, L Urbani, E Bertin, C Trevisan, P Gamba, AJ Burns, P De Coppi, M Pozzobon
Int J Mol Sci, 2018;19(5):. 2018
A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche
Authors: J Guarnerio, LM Mendez, N Asada, AV Menon, J Fung, K Berry, PS Frenette, K Ito, PP Pandolfi
Nat Commun, 2018;9(1):66. 2018
Mesenchymal stem cell carriers enhance antitumor efficacy of oncolytic adenoviruses in an immunocompetent mouse model
Authors: E Rincón, T Cejalvo, D Kanojia, A Alfranca, MÁ Rodríguez-, RAG Hoyos, Y Han, L Zhang, R Alemany, MS Lesniak, J García-Cas
Oncotarget, 2017;0(0):. 2017
Effects of collagen peptides intake on skin ageing and platelet release in chronologically aged mice revealed by cytokine array analysis
Authors: H Song, L Zhang, Y Luo, S Zhang, B Li
J. Cell. Mol. Med., 2017;0(0):. 2017
Downregulation of adaptor protein MyD88 compromises the angiogenic potential of B16 murine melanoma
Authors: LD Trucco, E Roselli, P Araya, NG Nuñez, HA Mena, JL Bocco, S Negrotto, M Maccioni
PLoS ONE, 2017;12(6):e0179897. 2017
Nuclear FAK and Runx1 cooperate to regulate IGFBP3, cell cycle progression and tumor growth
Authors: M Canel, A Byron, AH Sims, J Cartier, H Patel, MC Frame, VG Brunton, B Serrels, A Serrels
Cancer Res., 2017;0(0):. 2017
Integrin ?3/Akt signaling contributes to platelet-induced hemangioendothelioma growth
Authors: R Gu, X Sun, Y Chi, Q Zhou, H Xiang, DB Bosco, X Lai, C Qin, KF So, Y Ren, XM Chen
Sci Rep, 2017;7(1):6455. 2017
Fractalkine induces angiogenic potential in CX3CR1-expressing monocytes
Authors: Y Park, J Lee, JY Kwak, K Noh, E Yim, HK Kim, YJ Kim, HE Broxmeyer, JA Kim
J. Leukoc. Biol., 2017;0(0):. 2017
Inhibition of PHD3 by salidroside promotes neovascularization through cell-cell communications mediated by muscle-secreted angiogenic factors
Authors: J Zhang, V Kasim, YD Xie, C Huang, J Sisjayawan, A Dwi Ariyan, XS Yan, XY Wu, CP Liu, L Yang, M Miyagishi, SR Wu
Sci Rep, 2017;7(0):43935. 2017
Mdivi-1 induced acute changes in the angiogenic profile after ischemia-reperfusion injury in female mice
Authors: S Veeranki, SC Tyagi
Physiol Rep, 2017;5(11):. 2017
Regulatory T cells promote myelin regeneration in the central nervous system
Authors: Y Dombrowski, T O'Hagan, M Dittmer, R Penalva, SR Mayoral, P Bankhead, S Fleville, G Eleftheria, C Zhao, M Naughton, R Hassan, J Moffat, J Falconer, A Boyd, P Hamilton, IV Allen, A Kissenpfen, PN Moynagh, E Evergren, B Perbal, AC Williams, RJ Ingram, JR Chan, RJ Franklin, DC Fitzgerald
Nat. Neurosci, 2017;0(0):. 2017
Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
Autophagy, 2017;0(0):0. 2017
High-fat Diet Enhances Mammary Tumorigenesis and Pulmonary Metastasis and Alters Inflammatory and Angiogenic Profiles in MMTV-PyMT Mice
Authors: Sneha Sundaram
Anticancer Res, 2016;36(12):6279-6287. 2016
C-reactive protein can upregulate VEGF expression to promote ADSC-induced angiogenesis by activating HIF-1? via CD64/PI3k/Akt and MAPK/ERK signaling pathways
Stem Cell Res Ther, 2016;7(1):114. 2016
Wnt5a-Ror2 signaling in mesenchymal stem cells promotes proliferation of gastric cancer cells by activating CXCL16-CXCR6 axis.
Authors: Gosuke Takiguchi, Michiru Nishita, Kana Kurita, Yoshihiro Kakeji, Yasuhiro Minami
Cancer Science, 2016;0(0):1349-7006. 2016
Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma
PLoS ONE, 2016;11(5):e0156151. 2016
FAK regulates platelet extravasation and tumor growth after antiangiogenic therapy withdrawal
Authors: M Haemmerle, J Bottsford-, S Pradeep, ML Taylor, HJ Choi, JM Hansen, HJ Dalton, RL Stone, MS Cho, AM Nick, AS Nagaraja, T Gutschner, KM Gharpure, LS Mangala, R Rupaimoole, HD Han, B Zand, GN Armaiz-Pen, SY Wu, CV Pecot, AR Burns, G Lopez-Bere
J Clin Invest, 2016;0(0):. 2016
Granulocytic myeloid-derived suppressor cells promote angiogenesis in the context of multiple myeloma
Oncotarget, 2016;7(25):37931-37943. 2016
Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells
Authors: A Spiegel, MW Brooks, S Houshyar, F Reinhardt, M Ardolino, E Fessler, MB Chen, JA Krall, J DeCock, IK Zervantona, A Iannello, Y Iwamoto, V Cortez-Ret, RD Kamm, MJ Pittet, DH Raulet, RA Weinberg
Cancer Discov, 2016;0(0):. 2016
Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M
Stem Cell Reports, 2015;4(3):419-30. 2015
Monocyte Chemoattractant Protein-Induced Protein 1 (MCPIP1) Enhances Angiogenic and Cardiomyogenic Potential of Murine Bone Marrow-Derived Mesenchymal Stem Cells.
Authors: Labedz-Maslowska A, Lipert B, Berdecka D, Kedracka-Krok S, Jankowska U, Kamycka E, Sekula M, Madeja Z, Dawn B, Jura J, Zuba-Surma E
PLoS ONE, 2015;10(7):e0133746. 2015
Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.
Authors: Crouch E, Liu C, Silva-Vargas V, Doetsch F
J Neurosci, 2015;35(11):4528-39. 2015
Lysyl oxidase-like 2 (LOXL2) and E47 EMT factor: novel partners in E-cadherin repression and early metastasis colonization.
Authors: Canesin G, Cuevas E, Santos V, Lopez-Menendez C, Moreno-Bueno G, Huang Y, Csiszar K, Portillo F, Peinado H, Lyden D, Cano A
Oncogene, 2015;34(8):951-64. 2015
Application of VEGFA and FGF-9 enhances angiogenesis, osteogenesis and bone remodeling in type 2 diabetic long bone regeneration.
Authors: Wallner C, Schira J, Wagner J, Schulte M, Fischer S, Hirsch T, Richter W, Abraham S, Kneser U, Lehnhardt M, Behr B
PLoS ONE, 2015;10(3):e0118823. 2015
Lysophosphatidic acid alters the expression profiles of angiogenic factors, cytokines, and chemokines in mouse liver sinusoidal endothelial cells.
Authors: Chou C, Lai S, Ho C, Lin W, Chen C, Lee P, Peng F, Kuo S, Wu S, Lai H
PLoS ONE, 2015;10(3):e0122060. 2015
F-box protein FBXW7 inhibits cancer metastasis in a non-cell-autonomous manner.
Authors: Yumimoto, Kanae, Akiyoshi, Sayuri, Ueo, Hiroki, Sagara, Yasuaki, Onoyama, Ichiro, Ueo, Hiroaki, Ohno, Shinji, Mori, Masaki, Mimori, Koshi, Nakayama, Keiichi
J Clin Invest, 2015;125(2):621-35. 2015
Capillary force seeding of hydrogels for adipose-derived stem cell delivery in wounds.
Authors: Garg R, Rennert R, Duscher D, Sorkin M, Kosaraju R, Auerbach L, Lennon J, Chung M, Paik K, Nimpf J, Rajadas J, Longaker M, Gurtner G
Stem Cells Transl Med, 2014;3(9):1079-89. 2014
Pathophysiology of lung injury induced by common bile duct ligation in mice.
Authors: Shikata F, Sakaue T, Nakashiro K, Okazaki M, Kurata M, Okamura T, Okura M, Ryugo M, Nakamura Y, Yasugi T, Higashiyama S, Izutani H
PLoS ONE, 2014;9(4):e94550. 2014
c-Met inhibition in a HOXA9/Meis1 model of CN-AML.
Authors: Mulgrew N, Kettyle L, Ramsey J, Cull S, Smyth L, Mervyn D, Bijl J, Thompson A
Dev Dyn, 2014;243(1):172-81. 2014
Platelet secretion and hemostasis require syntaxin-binding protein STXBP5.
Authors: Ye S, Huang Y, Joshi S, Zhang J, Yang F, Zhang G, Smyth S, Li Z, Takai Y, Whiteheart S
J Clin Invest, 2014;124(10):4517-28. 2014
TLR9 agonist regulates angiogenesis and inhibits corneal neovascularization.
Authors: Wu J, Cui H, Dick A, Liu L
Am J Pathol, 2014;184(6):1900-10. 2014
Exosomes from retinal astrocytes contain antiangiogenic components that inhibit laser-induced choroidal neovascularization.
Authors: Hajrasouliha, Amir Rez, Jiang, Guomin, Lu, Qingxian, Lu, Huayi, Kaplan, Henry J, Zhang, Huang-Ge, Shao, Hui
J Biol Chem, 2013;288(39):28058-67. 2013
ADAMTS4 and its proteolytic fragments differentially affect melanoma growth and angiogenesis in mice.
Authors: Rao N, Ke Z, Liu H, Ho C, Kumar S, Xiang W, Zhu Y, Ge R
Int J Cancer, 2013;133(2):294-306. 2013
Differentiation state determines neural effects on microvascular endothelial cells.
Authors: Muffley L, Pan S, Smith A, Ga M, Hocking A, Gibran N
Exp Cell Res, 2012;318(16):2085-93. 2012
Vascular normalization by loss of Siah2 results in increased chemotherapeutic efficacy.
Authors: Wong CS, Sceneay J, House CM, Halse HM, Liu MC, George J, Hunnam TC, Parker BS, Haviv I, Ronai Z, Cullinane C, Bowtell DD, Moller A
Cancer Res., 2012;72(7):1694-704. 2012
ADAMTS5 functions as an anti-angiogenic and anti-tumorigenic protein independent of its proteoglycanase activity.
Authors: Kumar S, Sharghi-Namini S, Rao N, Ge R
Am J Pathol, 2012;181(3):1056-68. 2012
The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
Authors: Tran K, Risingsong R, Royce D
Cancer Prev Res (Phila), 2012;5(5):726-34. 2012
Antimycotic ciclopirox olamine in the diabetic environment promotes angiogenesis and enhances wound healing.
Authors: Ko SH, Nauta A, Morrison SD, Zhou H, Zimmermann A, Gurtner GC, Ding S, Longaker MT
PLoS ONE, 2011;6(11):e27844. 2011
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