R&D Systems' Human Apoptosis Array Kit (Catalog # ARY009) is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of expression of 35 apoptosis-related proteins in a single sample. No specialized equipment is necessary.
Proteome Profiler Mouse Apoptosis Array
R&D Systems | Catalog # ARY031
Contains 8 membranes - each spotted in duplicate with 21 different apoptosis antibodies
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Key Product Details
Species
Mouse
Product Summary for Proteome Profiler Mouse Apoptosis Array
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of mouse apoptosis-related proteins. Validated for analyte detection in cell lysates.
Troubleshooting Guide
Key Benefits
- Detects 21 mouse apoptosis-related proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Mouse Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.
Why Use an Antibody Array to Detect Apoptosis-Related Proteins?
Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
Kit Contents
- Rectangular 8-Well Multi-dish
- 8 Mouse Apoptosis Array nitrocellulose membranes spotted with 21 different antibodies to mouse apoptosis-related proteins
- Array Buffer 1
- Array Buffer 8
- Lysis Buffer 17
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse Apoptosis Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
.Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these apoptosis-related proteins in a single sample. | ||
|---|---|---|
| Bad | Fas/TNFRSF6/CD95 | MCL-1 |
| Bcl-2 | HIF-1α | p27/Kip1 |
| Bcl-x | HO-1/HMOX1/HSP32 | p53 |
| Cleaved Caspase-3 | HO-2/HMOX2 | SMAC/Diablo |
| Catalase | HSP27 | TNF RI/TNFRSF1A |
| Claspin | HSP60 | TRAIL R2/TNFRSF10B |
| Cytochrome c | HSP70/HSPA1A | XIAP |
Western blotting antibodies for analytes related to the Mouse Apoptosis Array Kit
| Analyte | Affinity Purified Polyclonal Antibodies (Total) | Monoclonal Antibodies (Total) | Labeled Antibodies (Total) |
|---|---|---|---|
| Bad | AF819 | MAB850 | |
| Bcl-2 | AF813 | MAB8272 | |
| MAB810 | |||
| Bcl-x | AF800 | ||
| Cleaved Caspase-3 | MAB835 | ||
| Catalase | AF3398 | ||
| Cytochrome c | MAB897 | ||
| MAB899 | |||
| Fas/TNFRSF6/CD95 | AF435 | BAF435 | |
| HIF-1α | AF1935 | MAB1536 | |
| HO-1/HMOX1/HSP32 | AF3776 | MAB3776 | |
| AF3169 | |||
| HO-2/HMOX2 | AF3170 | MAB3170 | |
| HSP27 | AF1580 | ||
| AF15801 | |||
| HSP60 | AF1800 | MAB1800 | |
| HSP70/HSPA1A | AF1663 | MAB1663 | |
| p27/Kip1 | AF2256 | MAB2256 | |
| p53 | AF1355 | MAB1355 | BAF1355 |
| HAF1355 | |||
| TNF RI/TNFRSF1A | AF-425-PB | MAB430 | BAF425 |
| MAB425 | |||
| TRAIL R2/TNFRSF10B | AF721 | MAB1540 | BAF721 |
| XIAP | AF8221 | MAB822 | BAF8221 |
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Scientific Data Images for Proteome Profiler Mouse Apoptosis Array
Expression of Mouse Apoptosis-related Proteins in Cell Lysate.
Figure 1A: A20 mouse rectal carcinoma cell lysate (650 μg).Figure 1B: SW/3T3 Swiss albino mouse embryo fi broblast cell lysate (300 μg).
Figure 1C: Ba/F3 mouse lymphocyte cell lysate (400 μg).
Figure 1D: Hepa 1-6 mouse liver epithelial cell lysate (650 μg).
Expression of Mouse Apoptosis-related Proteins in Cell Lysate.
Figure 2: Lysates from C2C12 mouse muscle myoblast cells were left untreated or treated with 1 μM STS for 3 hours.Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Apoptosis Array Kit
Additional Apoptosis Array Kit Products
Product Documents for Proteome Profiler Mouse Apoptosis Array
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Proteome Profiler Mouse Apoptosis Array
For research use only
Related Research Areas
Citations for Proteome Profiler Mouse Apoptosis Array
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