Proteome Profiler Mouse Apoptosis Array

Contains 8 membranes - each spotted in duplicate with 21 different apoptosis antibodies
Catalog # Availability Size / Price Qty
ARY031
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Proteome Profiler Mouse Apoptosis Array Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of mouse apoptosis-related proteins. Validated for analyte detection in cell lysates.

Troubleshooting Guide

Key Benefits

  • Detects 21 mouse apoptosis-related proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 

 

Principle of the Assay

The Proteome Profiler Mouse Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.

Why Use an Antibody Array to Detect Apoptosis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • Rectangular 8-Well Multi-dish
  • 8 Mouse Apoptosis Array nitrocellulose membranes spotted with 21 different antibodies to mouse apoptosis-related proteins
  • Array Buffer 1
  • Array Buffer 8
  • Lysis Buffer 17
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse Apoptosis Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

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Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 
Simultaneously detect the levels of these apoptosis-related proteins in a single sample.
Bad Fas/TNFRSF6/CD95 MCL-1
Bcl-2 HIF-1α p27/Kip1
Bcl-x HO-1/HMOX1/HSP32 p53
Cleaved Caspase-3 HO-2/HMOX2 SMAC/Diablo
Catalase HSP27 TNF RI/TNFRSF1A
Claspin HSP60 TRAIL R2/TNFRSF10B
Cytochrome c HSP70/HSPA1A XIAP

Western blotting antibodies for analytes related to the Mouse Apoptosis Array Kit

Analyte Affinity Purified Polyclonal Antibodies (Total) Monoclonal Antibodies (Total) Labeled Antibodies (Total)
Bad AF819 MAB850  
Bcl-2 AF813 MAB8272  
    MAB810  
Bcl-x AF800    
Cleaved Caspase-3   MAB835  
Catalase AF3398    
Cytochrome c   MAB897  
    MAB899  
Fas/TNFRSF6/CD95 AF435   BAF435
HIF-1α AF1935 MAB1536  
HO-1/HMOX1/HSP32 AF3776 MAB3776  
  AF3169    
HO-2/HMOX2 AF3170 MAB3170  
HSP27 AF1580    
  AF15801    
HSP60 AF1800 MAB1800  
HSP70/HSPA1A AF1663 MAB1663  
p27/Kip1 AF2256 MAB2256  
p53 AF1355 MAB1355 BAF1355
      HAF1355
TNF RI/TNFRSF1A AF-425-PB MAB430 BAF425
    MAB425  
TRAIL R2/TNFRSF10B AF721 MAB1540 BAF721
XIAP AF8221 MAB822 BAF8221

 

 
Data Examples
Figure 1A: A20 mouse rectal carcinoma cell lysate (650 μg).
Figure 1B: SW/3T3 Swiss albino mouse embryo fi broblast cell lysate (300 μg).
Figure 1C: Ba/F3 mouse lymphocyte cell lysate (400 μg).
Figure 1D: Hepa 1-6 mouse liver epithelial cell lysate (650 μg).
View Larger Image

Figure 1A: A20 mouse rectal carcinoma cell lysate (650 μg).
Figure 1B: SW/3T3 Swiss albino mouse embryo fi broblast cell lysate (300 μg).
Figure 1C: Ba/F3 mouse lymphocyte cell lysate (400 μg).
Figure 1D: Hepa 1-6 mouse liver epithelial cell lysate (650 μg).

Figure 2.
View Larger Image

Figure 2: Lysates from C2C12 mouse muscle myoblast cells were left untreated or treated with 1 μM STS for 3 hours.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Apoptosis Array Kit

R&D Systems' Human Apoptosis Array Kit (Catalog # ARY009) is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of expression of 35 apoptosis-related proteins in a single sample. No specialized equipment is necessary.

Alternate Names
Apoptosis Array Kit

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