Proteome Profiler Human Angiogenesis Array Kit
Proteome Profiler Human Angiogenesis Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma and saliva.
- Detects 55 human angiogenesis-related proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Human Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of bound analyte. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.
Why Use an Antibody Array to Detect Angiogenesis-Related Proteins?
Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
|Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample.|
|Tissue Factor/Factor III||IL-1 beta||Serpin E1/PAI-1|
|DPPIV/CD26||LAP (TGF-beta 1)||TIMP-1|
|FGF acidic||NRG1-beta 1||VEGF|
|FGF basic||Pentraxin 3||VEGF-C|
Assays for analytes represented in the Human Angiogenesis Antibody Array Kit
|Quantikine® ELISA Kits||DuoSet® ELISA Development Systems||Quantikine® HS (High Sensitivity) ELISA Kits||QuantiGlo® Chemiluminescent ELISA Kits|
|Tissue Factor/Factor III||DCF300||DY2339|
|PDGF-AB/PDGF-BB||DHD00C, DBB00||DY222, DY220|
Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
Profiling Angiogenesis-Related Proteins in Human Cancer Tissues. A) The Proteome Profiler Human Angiogenesis Antibody Array (Catalog # ARY007) was used to profile angiogenesis-related proteins in tissues from human prostate, ovarian, and breast cancers. B) Histogram profiles for select analytes were generated by quantifying the mean spot pixel densities from the array membrane using image software.
The Human Angiogenesis Array detects multiple analytes in cell culture supernates. A. THP-1 human acute monocytic leukemia cell line treated with 1 µg/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 8 hours and 1 µg/mL lipopolysaccharide (LPS) for 16 hours. B. HUVEC human umbilical vein endothelial cells treated with 10 mg/ml of LPS for 24 hours. 500 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
The Human Angiogenesis Array detects multiple analytes in tissue lysate, serum, and plasma. Representative membranes and selective quantification are shown for human ovarian cancer tissue lysate (A., 200 µg), serum (B., 100 µL), and EDTA plasma (C., 100 µL). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
Citations for Proteome Profiler Human Angiogenesis Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature
Authors: AG Kutikhin, AE Tupikin, VG Matveeva, DK Shishkova, LV Antonova, MR Kabilov, EA Velikanova
Cells, 2020;9(4):. 2020
Resveratrol Promotes Tumor Microvessel Growth via Endoglin and Extracellular Signal-Regulated Kinase Signaling Pathway and Enhances the Anticancer Efficacy of Gemcitabine against Lung Cancer
Authors: SH Qin, ATY Lau, ZL Liang, HW Tan, YC Ji, QH Zhong, XY Zhao, YM Xu
Cancers (Basel), 2020;12(4):. 2020
Natural Histogel-Based Bio-Scaffolds for Sustaining Angiogenesis in Beige Adipose Tissue
Authors: M Di Somma, W Schaafsma, E Grillo, M Vliora, E Dakou, M Corsini, C Ravelli, R Ronca, P Sakellario, J Vanparijs, B Castro, S Mitola
Cells, 2019;8(11):. 2019
Thrombin Preconditioning of Extracellular Vesicles Derived from Mesenchymal Stem Cells Accelerates Cutaneous Wound Healing by Boosting Their Biogenesis and Enriching Cargo Content
Authors: DK Sung, YS Chang, SI Sung, SY Ahn, WS Park
J Clin Med, 2019;8(4):. 2019
Harnessing Induced Essentiality: Targeting Carbonic Anhydrase IX and Angiogenesis Reduces Lung Metastasis of Triple Negative Breast Cancer Xenografts
Authors: EE Hedlund, PC McDonald, O Nemirovsky, S Awrey, LDE Jensen, S Dedhar
Cancers (Basel), 2019;11(7):. 2019
Telmisartan Inhibits Cell Proliferation and Tumor Growth of Esophageal Squamous Cell Carcinoma by Inducing S-Phase Arrest In Vitro and In Vivo
Authors: T Matsui, T Chiyo, H Kobara, S Fujihara, K Fujita, D Namima, M Nakahara, N Kobayashi, N Nishiyama, T Yachida, A Morishita, H Iwama, T Masaki
Int J Mol Sci, 2019;20(13):. 2019
TGFBI Protein Is Increased in the Urine of Patients with High-Grade Urothelial Carcinomas, and Promotes Cell Proliferation and Migration
Authors: K Lang, S Kahveci, N Bonberg, K Wichert, T Behrens, J Hovanec, F Roghmann, J Noldus, YC Tam, A Tannapfel, HU Käfferlein, T Brüning
Int J Mol Sci, 2019;20(18):. 2019
Homotypic and Heterotypic Activation of the Notch Pathway in Multiple Myeloma-Enhanced Angiogenesis: A Novel Therapeutic Target?
Authors: I Saltarella, MA Frassanito, A Lamanuzzi, A Brevi, P Leone, V Desantis, L Di Marzo, M Bellone, D Derudas, D Ribatti, R Chiaramont, MT Palano, A Neri, MA Mariggiò, R Fumarulo, F Dammacco, V Racanelli, A Vacca, R Ria
Neoplasia, 2019;21(1):93-105. 2019
The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method
Authors: D Kardos, M Simon, G Vácz, A Hinsenkamp, T Holczer, D Cseh, A Sárközi, K Szenthe, F Bánáti, S Szathmary, S Nehrer, O Kuten, M Masteling, Z Lacza, I Hornyák
Int J Mol Sci, 2019;20(3):. 2019
Characterization of the Angiogenic Potential of Human Regulatory Macrophages (Mreg) after Ischemia/Reperfusion Injury In Vitro
Authors: L Hummitzsch, K Zitta, R Rusch, J Cremer, M Steinfath, J Gross, F Fandrich, R Berndt, M Albrecht
Stem Cells Int, 2019;2019(0):3725863. 2019
Impaired Regeneration Potential in Urinary Stem Cells Diagnosed from the Patients with Diabetic Nephropathy
Authors: G Xiong, W Tang, D Zhang, D He, G Wei, A Atala, XJ Liang, AJ Bleyer, ME Bleyer, J Yu, JA Aloi, JX Ma, CM Furdui, Y Zhang
Theranostics, 2019;9(14):4221-4232. 2019
The efficacy of conditioned medium released by tonsil-derived mesenchymal stem cells in a chronic murine colitis model
Authors: KE Lee, SA Jung, YH Joo, EM Song, CM Moon, SE Kim, I Jo
PLoS ONE, 2019;14(12):e0225739. 2019
Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p
Authors: C Gao, Z Gong, D Wang, J Huang, Y Qian, M Nie, W Jiang, X Liu, H Luo, J Yuan, T Xiang, S An, W Quan, H Wei, J Zhang, R Jiang
Aging (Albany NY), 2019;11(24):12147-12164. 2019
B-Cell Activating Factor Enhances Hepatocyte-Driven Angiogenesis via B-Cell CLL/Lymphoma 10/Nuclear Factor-KappaB Signaling during Liver Regeneration
Authors: CH Chou, CM Ho, SL Lai, CN Chen, YM Wu, CT Shun, WF Wen, HS Lai
Int J Mol Sci, 2019;20(20):. 2019
Patient-Specific iPSC Model of a Genetic Vascular Dementia Syndrome Reveals Failure of Mural Cells to Stabilize Capillary Structures
Authors: J Kelleher, A Dickinson, S Cain, Y Hu, N Bates, A Harvey, J Ren, W Zhang, FC Moreton, KW Muir, C Ward, RM Touyz, P Sharma, Q Xu, SJ Kimber, T Wang
Stem Cell Reports, 2019;13(5):817-831. 2019
Targeting angiogenesis in multiple myeloma by the VEGF and HGF blocking DARPinï¿½ protein MP0250: a preclinical study
Authors: L Rao, K De Veirman, D Giannico, I Saltarella, V Desantis, MA Frassanito, AG Solimando, D Ribatti, M Prete, A Harstrick, U Fiedler, H De Raeve, V Racanelli, K Vanderkerk, A Vacca
Oncotarget, 2018;9(17):13366-13381. 2018
Mitochondrial dysfunction is a key determinant of the rare disease lymphangioleiomyomatosis and provides a novel therapeutic target
Authors: EMM Abdelwahab, S Pal, K Kvell, V Sarosi, P Bai, R Rue, V Krymskaya, D McPhail, A Porter, JE Pongracz
Oncogene, 2018;0(0):. 2018
Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells
Authors: A Kim, JY Ma
Front Pharmacol, 2018;9(0):1455. 2018
Protein array profiling of circulating angiogenesis-related factors during bevacizumab containing treatment in metastatic colorectal cancer
Authors: H Hagman, PO Bendahl, J Lidfeldt, M Belting, A Johnsson
PLoS ONE, 2018;13(12):e0209838. 2018
Plocabulin, a novel tubulin-binding agent, inhibits angiogenesis by modulation of microtubule dynamics in endothelial cells
Authors: CM Galmarini, M Martin, BP Bouchet, MJ Guillen-Na, M Martínez-D, JF Martinez-L, A Akhmanova, P Aviles
BMC Cancer, 2018;18(1):164. 2018
A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells
Authors: HK Kim, SG Lee, SW Lee, BJ Oh, JH Kim, JA Kim, G Lee, JD Jang, YA Joe
Stem Cells, 2018;0(0):. 2018
Multipotent Adult Progenitor Cells Support Lymphatic Regeneration at Multiple Anatomical Levels during Wound Healing and Lymphedema
Authors: M Beerens, XL Aranguren, B Hendrickx, W Dheedene, T Dresselaer, U Himmelreic, C Verfaillie, A Luttun
Sci Rep, 2018;8(1):3852. 2018
Increased platelet factor 4 and aberrant permeability of follicular fluid in PCOS
Authors: CC Huang, CH Chou, SU Chen, HN Ho, YS Yang, MJ Chen
J. Formos. Med. Assoc., 2018;0(0):. 2018
Nintedanib inhibits growth of human prostate carcinoma cells by modulating both cell cycle and angiogenesis regulators
Authors: RF da Silva, D Dhar, K Raina, D Kumar, R Kant, VHA Cagnon, C Agarwal, R Agarwal
Sci Rep, 2018;8(1):9540. 2018
hiPSC-derived neural stem cells from patients with schizophrenia induce an impaired angiogenesis
Authors: BS Casas, G Vitória, MN do Costa, R Madeiro da, P Trindade, R Maciel, N Navarrete, SK Rehen, V Palma
Transl Psychiatry, 2018;8(1):48. 2018
Human dental pulp stromal cell conditioned medium alters endothelial cell behavior
Authors: MA Gharaei, Y Xue, K Mustafa, SA Lie, I Fristad
Stem Cell Res Ther, 2018;9(1):69. 2018
Stimulation of Interferon-Stimulated Gene 20 by Thyroid Hormone Enhances Angiogenesis in Liver Cancer
Authors: SL Lin, SM Wu, IH Chung, YH Lin, CY Chen, HC Chi, TK Lin, CT Yeh, KH Lin
Neoplasia, 2018;20(1):57-68. 2018
Switch in Laminin Î²2 to Laminin Î²1 Isoforms During Aging Controls Endothelial Cell Functions
Authors: JUG Wagner, E Chavakis, EM Rogg, M Muhly-Rein, SF Glaser, S Günther, D John, F Bonini, AM Zeiher, L Schaefer, MJ Hannocks, RA Boon, S Dimmeler
Arterioscler. Thromb. Vasc. Biol., 2018;0(0):. 2018
Extracellular Matrix composition modulates PDAC parenchymal and stem cell plasticity and behavior through the secretome
Authors: G Biondani, K Zeeberg, MR Greco, S Cannone, I Dando, ED Pozza, M Mastrodona, S Forciniti, V Casavola, M Palmieri, SJ Reshkin, RA Cardone
FEBS J., 2018;0(0):. 2018
Employing mesenchymal stem cells to support tumor-targeted delivery of extracellular vesicle (EV)-encapsulated microRNA-379
Authors: KP O'Brien, S Khan, KE Gilligan, H Zafar, P Lalor, C Glynn, C O'Flathart, H Ingoldsby, P Dockery, A De Bhulbh, JR Schweber, K St John, M Leahy, JM Murphy, WM Gallagher, T O'Brien, MJ Kerin, RM Dwyer
Oncogene, 2018;0(0):. 2018
Intranasal delivery of VEGF enhances compensatory lung growth in mice
Authors: DT Dao, JT Vuong, L Anez-Busti, A Pan, PD Mitchell, GL Fell, MA Baker, DR Bielenberg, M Puder
PLoS ONE, 2018;13(6):e0198700. 2018
SCO-spondin oligopeptide inhibits angiogenesis in glioblastoma
Authors: R Bibes, S Gobron, F Vincent, C Mélin, N Vedrenne, A Perraud, F Labrousse, MO Jauberteau, F Lalloué
Oncotarget, 2017;8(49):85969-85983. 2017
Antitumor activity of miR-34a in peritoneal mesothelioma relies on c-MET and AXL inhibition: persistent activation of ERK and AKT signaling as a possible cytoprotective mechanism
Authors: R El Bezawy, M De Cesare, M Pennati, M Deraco, P Gandellini, V Zuco, N Zaffaroni
J Hematol Oncol, 2017;10(1):19. 2017
Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis
Authors: Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng
Stem Cells, 2017;0(0):. 2017
Combination curcumin and (-)-epigallocatechin-3-gallate inhibits colorectal carcinoma microenvironment-induced angiogenesis by JAK/STAT3/IL-8 pathway
Authors: G Jin, Y Yang, K Liu, J Zhao, X Chen, H Liu, R Bai, X Li, Y Jiang, X Zhang, J Lu, Z Dong
Oncogenesis, 2017;6(10):e384. 2017
Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival
Authors: RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin
Oncotarget, 2017;8(37):62081-62098. 2017
Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
Cancer Res., 2017;0(0):. 2017
Neuroprotective Effects of Human Mesenchymal Stem Cells and Platelet-Derived Growth Factor on Human Retinal Ganglion Cells
Authors: A Osborne, J Sanderson, KR Martin
Stem Cells, 2017;0(0):. 2017
Sonic hedgehog (SHH) signaling improves the angiogenic potential of Wharton's jelly-derived mesenchymal stem cellsï¿½(WJ-MSC)
Authors: G Zavala, CP Prieto, AA Villanueva, V Palma
Stem Cell Res Ther, 2017;8(1):203. 2017
NF-?B Signaling Activation Induced by Chloroquine Requires Autophagosome, p62 Protein, and c-Jun N-terminal Kinase (JNK) Signaling and Promotes Tumor Cell Resistance
Authors: S Yang, L Qiang, A Sample, P Shah, YY He
J. Biol. Chem., 2017;292(8):3379-3388. 2017
Metabolically conditioned media derived from bone marrow stromal cells or human skin fibroblasts act as effective chemoattractants for mesenchymal stem cells
Authors: A Gabrielyan, E Neumann, M Gelinsky, A Rösen-Wolf
Stem Cell Res Ther, 2017;8(1):212. 2017
CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect
Authors: S Sakimoto, V Marchetti, E Aguilar, K Lee, Y Usui, S Murinello, F Bucher, JK Trombley, R Fallon, R Wagey, C Peters, EL Scheppke, PD Westenskow, M Friedlande
JCI Insight, 2017;2(2):e89906. 2017
Cell Spheroids with Enhanced Aggressiveness to Mimic Human Liver Cancer In Vitro and In Vivo
Authors: HR Jung, HM Kang, JW Ryu, DS Kim, KH Noh, ES Kim, HJ Lee, KS Chung, HS Cho, NS Kim, DS Im, JH Lim, CR Jung
Sci Rep, 2017;7(1):10499. 2017
15-Lipoxygenase-1 re-expression in colorectal cancer alters endothelial cell features through enhanced expression of TSP-1 and ICAM-1
Authors: S Tunçer, AG Ke?kü?, M Çolako?lu, I Çimen, C Yener, Ö Konu, S Banerjee
Cell. Signal., 2017;39(0):44-54. 2017
Targeted nanocomplex carrying siRNA against MALAT1 sensitizes glioblastoma to temozolomide
Authors: SS Kim, JB Harford, M Moghe, A Rait, KF Pirollo, EH Chang
Nucleic Acids Res., 2017;0(0):. 2017
Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
Autophagy, 2017;0(0):0. 2017
VLDL and LDL, but not HDL, promote breast cancer cell proliferation, metastasis and angiogenesis
Authors: Chun-Wun Lu
Cancer Lett, 2016;388(0):130-138. 2016
Identification of neuronal and angiogenic growth factors in an in vitroblood-brain barrier model system: Relevance in barrier integrity and tight junction formation and complexity
Authors: Christian Freese
Microvasc. Res, 2016;0(0):. 2016
Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
Authors: Gabriela Odent Grig
J Tissue Eng Regen Med, 2016;0(0):. 2016
Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment
PLoS ONE, 2016;11(7):e0159013. 2016
The Development of an Angiogenic Protein Signature in Ovarian Cancer Ascites as a Tool for Biologic and Prognostic Profiling
Authors: Sofia-Paraskevi Trachana
PLoS ONE, 2016;11(6):e0156403. 2016
IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
Authors: Matthew N Svalina
Sci Rep, 2016;6(0):27012. 2016
The Angiogenic Secretome in VEGF overexpressing Breast Cancer Xenografts
Sci Rep, 2016;6(0):39460. 2016
Hypoxia-induced responses by endothelial colony-forming cells are modulated by placental growth factor
Stem Cell Res Ther, 2016;7(1):173. 2016
A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs): An Achievement of Significant Morphological, Numerical and Functional Recovery
PLoS ONE, 2016;11(11):e0166639. 2016
Ethanol extract of Lophatheri Herba exhibits anti-cancer activity in human cancer cells by suppression of metastatic and angiogenic potential
Sci Rep, 2016;6(0):36277. 2016
Lysyl Oxidase (LOX) transcriptionally regulates SNAI2 expression and TIMP4 secretion in human cancers
Authors: M Boufraqech, L Zhang, N Nilubol, SM Sadowski, S Kotian, MM Quezado, E Kebebew
Clin Cancer Res, 2016;0(0):. 2016
Prostacyclin-producing human mesenchymal cells target H19 lncRNA to augment endogenous progenitor function in hindlimb ischaemia
Authors: Y Deng, Z Yang, T Terry, S Pan, DG Woodside, J Wang, K Ruan, JT Willerson, RA Dixon, Q Liu
Nat Commun, 2016;7(0):11276. 2016
RECK controls breast cancer metastasis by modulating a convergent, STAT3-dependent neoangiogenic switch.
Authors: Walsh, L A, Roy, D M, Reyngold, M, Giri, D, Snyder, A, Turcan, S, Badwe, C R, Lyman, J, Bromberg, J, King, T A, Chan, T A
Oncogene, 2015;34(17):2189-203. 2015
Inhibition of IGF-1-Mediated Cellular Migration and Invasion by Migracin A in Ovarian Clear Cell Carcinoma Cells.
Authors: Ukaji T, Lin Y, Banno K, Okada S, Umezawa K
PLoS ONE, 2015;10(9):e0137663. 2015
Deficiency of endothelium-specific transcription factor Sox17 induces intracranial aneurysm.
Authors: Lee S, Kim I, Ahn J, Woo D, Kim S, Song S, Koh G, Kim H, Jeon B, Kim I
, 2015;0(0):. 2015
Signaling via PI3K/FOXO1A pathway modulates formation and survival of human embryonic stem cell-derived endothelial cells.
Authors: Merkely B, Gara E, Lendvai Z, Skopal J, Leja T, Zhou W, Kosztin A, Varady G, Mioulane M, Bagyura Z, Nemeth T, Harding S, Foldes G
Stem Cells Dev, 2015;24(7):869-78. 2015
Exosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts.
Authors: Paggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim Y, Adam J, Lichter P, Solary E, Berchem G, Moussay E
Blood, 2015;126(9):1106-17. 2015
Identification of Novel Biomarkers for Metastatic Colorectal Cancer Using Angiogenesis-Antibody Array and Intracellular Signaling Array.
Authors: Chung S, Dwabe S, Elshimali Y, Sukhija H, Aroh C, Vadgama J
PLoS ONE, 2015;10(8):e0134948. 2015
Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli?
Authors: Spampinato S, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff R
PLoS ONE, 2015;10(7):e0133392. 2015
Intratumoral CD14+ Cells and Circulating CD14+HLA-DRlo/neg Monocytes Correlate with Decreased Survival in Patients with Clear Cell Renal Cell Carcinoma.
Authors: Gustafson M, Lin Y, Bleeker J, Warad D, Tollefson M, Crispen P, Bulur P, Harrington S, Laborde R, Gastineau D, Leibovich B, Cheville J, Kwon E, Dietz A
Clin Cancer Res, 2015;21(18):4224-33. 2015
CoREST1 promotes tumor formation and tumor stroma interactions in a mouse model of breast cancer.
Authors: Mazumdar, Sohini, Arendt, Lisa M, Phillips, Sarah, Sedic, Maja, Kuperwasser, Charlott, Gill, Grace
PLoS ONE, 2015;10(3):e0121281. 2015
Sourcing of an alternative pericyte-like cell type from peripheral blood in clinically relevant numbers for therapeutic angiogenic applications.
Authors: Blocki A, Wang Y, Koch M, Goralczyk A, Beyer S, Agarwal N, Lee M, Moonshi S, Dewavrin J, Peh P, Schwarz H, Bhakoo K, Raghunath M
Mol Ther, 2015;23(3):510-22. 2015
PTEN expression is involved in the invasive properties of HNSCC: a key protein to consider in locoregional recurrence.
Authors: Mriouah J, Boura C, Gargouri M, Plenat F, Faivre B
Int J Oncol, 2014;44(3):709-16. 2014
Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway.
Authors: Kryza T, Achard C, Parent C, Marchand-Adam S, Guillon-Munos A, Iochmann S, Korkmaz B, Respaud R, Courty Y, Heuze-Vourc'h N
FASEB J, 2014;28(2):740-51. 2014
Lysophosphatidic acid enhanced the angiogenic capability of human chondrocytes by regulating Gi/NF-kB-dependent angiogenic factor expression.
Authors: Chuang, Yi-Wen, Chang, Wen-Ming, Chen, Kai-Hua, Hong, Chang-Ze, Chang, Pey-Jium, Hsu, Hung-Chi
PLoS ONE, 2014;9(5):e95180. 2014
Aberrant chloride intracellular channel 4 expression contributes to endothelial dysfunction in pulmonary arterial hypertension.
Authors: Wojciak-Stothard B, Abdul-Salam V, Lao K, Tsang H, Irwin D, Lisk C, Loomis Z, Stenmark K, Edwards J, Yuspa S, Howard L, Edwards R, Rhodes C, Gibbs J, Wharton J, Zhao L, Wilkins M
Circulation, 2014;129(17):1770-80. 2014
Lymphatic endothelial cells support tumor growth in breast cancer.
Authors: Lee, Esak, Pandey, Niranjan, Popel, Aleksand
Sci Rep, 2014;4(0):5853. 2014
Peripheral Blood-Derived Mesenchymal Stromal Cells Promote Angiogenesis via Paracrine Stimulation of Vascular Endothelial Growth Factor Secretion in the Equine Model.
Authors: Bussche L, Van de Walle G
Stem Cells Transl Med, 2014;3(12):1514-25. 2014
Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
Nat Commun, 2014;5(0):4715. 2014
Ionizing radiation induces tumor cell lysyl oxidase secretion.
Authors: Shen C, Sharma A, Vuong D, Erler J, Pruschy M, Broggini-Tenzer A
BMC Cancer, 2014;14(0):532. 2014
Mesenchymal stromal cells: inhibiting PDGF receptors or depleting fibronectin induces mesodermal progenitors with endothelial potential.
Authors: Ball S, Worthington J, Canfield A, Merry C, Kielty C
Stem Cells, 2014;32(3):694-705. 2014
Sortilin mediates the release and transfer of exosomes in concert with two tyrosine kinase receptors.
Authors: Wilson C, Naves T, Vincent F, Melloni B, Bonnaud F, Lalloue F, Jauberteau M
J Cell Sci, 2014;127(0):3983-97. 2014
Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.
Authors: Hutzen, Brian, Bid, Hemant K, Houghton, Peter J, Pierson, Christop, Powell, Kimerly, Bratasz, Anna, Raffel, Corey, Studebaker, Adam W
BMC Cancer, 2014;14(0):206. 2014
Histone deacetylase-3 mediates positive feedback relationship between anaphylaxis and tumor metastasis.
Authors: Eom S, Kim Y, Park D, Lee H, Lee Y, Choe J, Kim Y, Jeoung D
J Biol Chem, 2014;289(17):12126-44. 2014
Surgical sutures filled with adipose-derived stem cells promote wound healing.
Authors: Reckhenrich A, Kirsch B, Wahl E, Schenck T, Rezaeian F, Harder Y, Foehr P, Machens H, Egana J
PLoS ONE, 2014;9(3):e91169. 2014
Staphylococcal nuclease domain containing-1 (SND1) promotes migration and invasion via angiotensin II type 1 receptor (AT1R) and TGFbeta signaling.
Authors: Santhekadur P, Akiel M, Emdad L, Gredler R, Srivastava J, Rajasekaran D, Robertson C, Mukhopadhyay N, Fisher P, Sarkar D
FEBS Open Bio, 2014;4(0):353-61. 2014
Extract of Cordyceps militaris inhibits angiogenesis and suppresses tumor growth of human malignant melanoma cells.
Authors: Ruma I, Putranto E, Kondo E, Watanabe R, Saito K, Inoue Y, Yamamoto K, Nakata S, Kaihata M, Murata H, Sakaguchi M
Int J Oncol, 2014;45(1):209-18. 2014
Ultraviolet B preconditioning enhances the hair growth-promoting effects of adipose-derived stem cells via generation of reactive oxygen species.
Stem Cells Dev, 2013;22(1):158-68. 2013
Fibroblasts derived from human pluripotent stem cells activate angiogenic responses in vitro and in vivo.
Authors: Shamis Y, Silva E, Hewitt K, Brudno Y, Levenberg S, Mooney D, Garlick J
PLoS ONE, 2013;8(12):e83755. 2013
A metabolic shift favoring sphingosine 1-phosphate at the expense of ceramide controls glioblastoma angiogenesis.
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A novel serum protein signature associated with resistance to epidermal growth factor receptor tyrosine kinase inhibitors in head and neck squamous cell carcinoma.
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Inhibition of TGF-ÃŽÂ² signaling by the fungal lactones (S)-curvularin, dehydrocurvularin, oxacyclododecindione and galiellalactone.
Cytokine, 2013;61(1):285-96. 2013
Functional and molecular characterization of ex vivo cultured epiretinal membrane cells from human proliferative diabetic retinopathy.
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Cyclin-dependent kinase 8 mediates chemotherapy-induced tumor-promoting paracrine activities.
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Tumor-initiating cells of various tumor types exhibit differential angiogenic properties and react differently to antiangiogenic drugs.
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Primary tumor hypoxia recruits CD11b+/Ly6Cmed/Ly6G+ immune suppressor cells and compromises NK cell cytotoxicity in the premetastatic niche.
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
What are the associated pathways each factor is involved in?
We do not have specific angiogenesis-related categorization of the target for this array, but we do have some informative material available in our website:
Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?
The proteinase inhibitors are recommended for cell and tissue lysates only. You do not need them for cell culture supernates.
Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
Which gene of the VEGF family is detected on E19 and E20
The VEGF spots are detecting VEGF-A.
Does the Human Angiogenesis Proteome Profiler Array cross-react with other species like equines?
We have not tested this kit with equine samples. However, when looking at the cross reactivity with our Proteome Profiler Arrays, there are a few things to keep in mind. When the arrays are being designed, we look for the best antibodies that will work for the species stated. This does not mean there will not be any cross reactivity between species but it does suggest that the assay would not perform equally between species. While it is possible that some of the antibodies may detect the equine protein, different antibodies cross-react at different levels.
It is also important to note that the Proteome Profiler Arrays are designed to be used as a screening tool. This may be a good place to start if you are trying determine what may be having an increase or a decrease in signal. We recommend running one untreated sample and comparing them to your treated samples. Even though you may not have the exact same cross reactivity with each analyte, you may be able to detect the increase or decrease from your samples.
We have searched for citations that may have referenced using this kit with equine samples and were able to find 1. Here is the link to it: https://www.ncbi.nlm.nih.gov/pubmed/25313202.
Reviews for Proteome Profiler Human Angiogenesis Array Kit
Average Rating: 4.9 (Based on 8 Reviews)
Have you used Proteome Profiler Human Angiogenesis Array Kit?
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Worked well to look at THP-1 angiogenesis factors.
It worked well in cell culture supernatants using 125 ug of total protein per membrane.
Used it on Conditioned medium collected from Breast Cancer Cells treated with Angiotensin II to look into its effect on Angiogenesis. Angiogenesis array worked really well and very easy to use. It provided some new promising gene targets which we did not know before.
Human Angiogenesis Antibody Array was used to evaluate mesenchymal stem cells secretion profiles when seeded in various extracellular matrices. Great tool that ganarates lots of data!