Proteome Profiler Human Angiogenesis Array Kit

Contains 4 membranes - each spotted in duplicate with 55 different angiogenesis antibodies
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Profiling Angiogenesis-Related Proteins in Human Cancer Tissues.
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Proteome Profiler Human Angiogenesis Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma and saliva.

Troubleshooting Guide

Key Benefits

  • Detects 55 human angiogenesis-related proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are rquired.  Refer to protocol.

Principle of the Assay

The Proteome Profiler Human Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of bound analyte. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.

Why Use an Antibody Array to Detect Angiogenesis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.



Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Antibody Detection Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample.
Angiopoietin-1 HB-EGF Persephin
Angiopoietin-2 HGF CXCL4/PF4
Angiostatin/Plasminogen IGFBP-1 PlGF
Amphiregulin IGFBP-2 Prolactin
Artemin IGFBP-3 Serpin B5/Maspin
Tissue Factor/Factor III IL-1 beta Serpin E1/PAI-1
CXCL16 CXCL8/IL-8 Serpin F1/PEDF
DPPIV/CD26 LAP (TGF-beta 1) TIMP-1
EGF Leptin TIMP-4
EG-VEGF CCL2/MCP-1 Thrombospondin-1
Endoglin/CD105 CCL3/MIP-1 alpha Thrombospondin-2
Endostatin/Collagen XVIII MMP-8 uPA
Endothelin-1 MMP-9 Vasohibin
FGF acidic NRG1-beta 1 VEGF
FGF basic Pentraxin 3 VEGF-C


Assays for analytes represented in the Human Angiogenesis Antibody Array Kit

  Quantikine® ELISA Kits DuoSet® ELISA Development Systems Quantikine® HS (High Sensitivity) ELISA Kits QuantiGlo® Chemiluminescent ELISA Kits
Activin A DAC00B DY338    
ADAMTS-1   DY2197    
Angiogenin DAN00 DY265    
Angiopoietin-1 DANG10 DY923    
Angiopoietin-2 DANG20 DY623    
Amphiregulin   DY262    
Artemin   DY2589    
Tissue Factor/Factor III DCF300 DY2339    
CXCL16 DCX160 DY1164    
DPPIV/CD26   DY1180    
EGF DEG00 DY236    
EG-VEGF   DY1209    
Endoglin DNDG00 DY1097    
Endostatin/Collagen XVII DNST0 DY1098    
Endothelin-1 DET100     QET00B
FGF acidic DFA00B DY232    
FGF basic DFB50 DY233 HSFB00D  
FGF-4   DY235    
FGF-7/KGF DKG00 DY251    
GDNF   DY212    
HB-EGF   DY259    
HGF DHG00 DY294    
IGFBP-1   DY871    
IGFBP-2 DGB200 DY674    
IGFBP-3 DGB300 DY675    
IL-1 beta DLB50 DY201 HSLB00D QLB00B
CXCL8/IL-8 D8000C DY208 HS800 Q8000B
TGF-beta1 (LAP) DB100B DY240    
Leptin DLP00 DY398    
CCL2/MCP-1 DCP00 DY279    
CCL3/MIP-1 alpha DMA00 DY270    
MMP-8 DMP800 DY908    
MMP-9 DMP900 DY911    
NRG1-beta1   DY377    
Pentraxin 3 DPTX30 DY1826    
PDGF-AA DAA00B DY221    
PDGF-AB/PDGF-BB DHD00C, DBB00 DY222, DY220    
CXCL4/PF4   DY795    
PlGF DPG00 DY264    
Prolactin DPRL00 DY682    
Serpin B5/Maspin        
Serpin E1/PAI-1 DSE100 DY1786    
Serpin F1/PEDF        
TIMP-1 DTM100 DY970    
TIMP-4 DTM400 DY974    
Thrombospondin-1 DTSP10 DY3074    
Thrombospondin-2 DTSP20 DY1635    
uPA DUPA00 DY1310    
VEGF-C DVEC00 DY752B    


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Scientific Data

Profiling Angiogenesis-Related Proteins in Human Cancer Tissues. A) The Proteome Profiler Human Angiogenesis Antibody Array (Catalog # ARY007) was used to profile angiogenesis-related proteins in tissues from human prostate, ovarian, and breast cancers. B) Histogram profiles for select analytes were generated by quantifying the mean spot pixel densities from the array membrane using image software.

The Human Angiogenesis Array detects multiple analytes in cell culture supernates. A. THP-1 human acute monocytic leukemia cell line treated with 1 µg/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 8 hours and 1 µg/mL lipopolysaccharide (LPS) for 16 hours. B. HUVEC human umbilical vein endothelial cells treated with 10 mg/ml of LPS for 24 hours. 500 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

The Human Angiogenesis Array detects multiple analytes in tissue lysate, serum, and plasma. Representative membranes and selective quantification are shown for human ovarian cancer tissue lysate (A., 200 µg), serum (B., 100 µL), and EDTA plasma (C., 100 µL). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Citations for Proteome Profiler Human Angiogenesis Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

182 Citations: Showing 1 - 10
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  1. The contribution of inflammatory astrocytes to BBB impairments in a brain-chip model of Parkinson's disease
    Authors: de Rus Jacquet, A;Alpaugh, M;Denis, HL;Tancredi, JL;Boutin, M;Decaestecker, J;Beauparlant, C;Herrmann, L;Saint-Pierre, M;Parent, M;Droit, A;Breton, S;Cicchetti, F;
    Nature communications  2023-06-20
  2. Augmenting the Angiogenic Profile and Functionality of Cord Blood Endothelial Colony-Forming Cells by Indirect Priming with Bone-Marrow-Derived Mesenchymal Stromal Cells
    Authors: Bansal, A;Singh, A;Nag, TC;Sharma, D;Garg, B;Bhatla, N;Choudhury, SD;Ramakrishnan, L;
    Biomedicines  2023-05-05
  3. Liver X Receptor Agonist Inhibits Oxidized Low-Density Lipoprotein Induced Choroidal Neovascularization via the NF-kappaB Signaling Pathway
    Authors: T Wu, X Wei, K Dang, M Tao, B Lv, T Chen, Z Zhang, J Zhou, H Du
    Oncogene, 2023-02-20;12(4):.  2023-02-20
  4. Urokinase-type plasminogen activator blockade ameliorates experimental colitis in mice
    Authors: Y Kida, T Okahisa, Y Sato, M Bando, S Fujimoto, B Ma, T Nakagawa, T Kawaguchi, F Nakamura, K Okamoto, H Miyamoto, M Sogabe, K Tsuneyama, T Takayama
    Scientific Reports, 2023-02-18;13(1):2899.  2023-02-18
  5. Characterization of large extracellular vesicles (L-EV) derived from human regulatory macrophages (Mreg): novel mediators in wound healing and angiogenesis?
    Authors: M Albrecht, L Hummitzsch, R Rusch, K He beta, M Steinfath, J Cremer, F Lichte, F Fändrich, R Berndt, K Zitta
    Journal of Translational Medicine, 2023-01-30;21(1):61.  2023-01-30
  6. The D405N Mutation in the Spike Protein of SARS-CoV-2 Omicron BA.5 Inhibits Spike/Integrins Interaction and Viral Infection of Human Lung Microvascular Endothelial Cells
    Authors: A Bugatti, F Filippini, S Messali, M Giovanetti, C Ravelli, A Zani, M Ciccozzi, A Caruso, F Caccuri
    Viruses, 2023-01-24;15(2):.  2023-01-24
  7. Inhibition of GPR39 restores defects in endothelial cell-mediated neovascularization under the duress of chronic hyperglycemia: Evidence for regulatory roles of the sonic hedgehog signaling axis
    Authors: SP Meda Venka, H Li, L Xu, JY Koh, H Nguyen, M Minjares, C Li, A Kowluru, G Milligan, JM Wang
    Proceedings of the National Academy of Sciences of the United States of America, 2022-12-27;120(1):e2208541120.  2022-12-27
  8. RNF213 Loss-of-Function Promotes Angiogenesis of Cerebral Microvascular Endothelial Cells in a Cellular State Dependent Manner
    Authors: V Roy, A Brodeur, L Touzel Des, N Dupré, F Gros-Louis
    Cells, 2022-12-24;12(1):.  2022-12-24
  9. Secretome from human adipose-derived mesenchymal stem cells promotes blood vessel formation and pericyte coverage in experimental skin repair
    Authors: BM Silveira, TO Ribeiro, RS Freitas, ACO Carreira, MS Gonçalves, M Sogayar, R Meyer, A Birbrair, V Fortuna
    PLoS ONE, 2022-12-19;17(12):e0277863.  2022-12-19
  10. PARP Inhibitor Inhibits the Vasculogenic Mimicry through a NF-kappaB-PTX3 Axis Signaling in Breast Cancer Cells
    Authors: J Chivot, N Ferrand, A Fert, P Van Dreden, R Morichon, M Sabbah
    International Journal of Molecular Sciences, 2022-12-18;23(24):.  2022-12-18
  11. Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue
    Authors: S Freitas-Ri, GS Diogo, C Oliveira, A Martins, TH Silva, M Jarnalo, R Horta, RL Reis, RP Pirraco
    Marine Drugs, 2022-09-30;20(10):.  2022-09-30
  12. The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO2NPs) Using Caco-2 Cells
    Authors: OM David, KL Lategan, MF de Cortale, EJ Pool
    Biomolecules, 2022-09-21;12(10):.  2022-09-21
  13. MIIP functions as a novel ligand for ITGB3 to inhibit angiogenesis and tumorigenesis of triple-negative breast cancer
    Authors: Y Gao, Y Fang, Y Huang, R Ma, X Chen, F Wang, X Pei, Y Gao, X Chen, X Liu, J Shan, P Li
    Cell Death & Disease, 2022-09-21;13(9):810.  2022-09-21
  14. Overexpression of mir-135b and mir-210 in mesenchymal stromal cells for the enrichment of extracellular vesicles with angiogenic factors
    Authors: JMF Vieira, LN Zamproni, CHC Wendt, K Rocha de M, RS Lindoso, S Won Han
    PLoS ONE, 2022-08-16;17(8):e0272962.  2022-08-16
  15. Angiogenic Potential of Co-Cultured Human Umbilical Vein Endothelial Cells and Adipose Stromal Cells in Customizable 3D Engineered Collagen Sheets
    Authors: P Nessbach, S Schwarz, TD Becke, H Clausen-Sc, HG Machens, S Sudhop
    Journal of functional biomaterials, 2022-07-29;13(3):.  2022-07-29
  16. WNK1 collaborates with TGF-beta in endothelial cell junction turnover and angiogenesis
    Authors: AB Jaykumar, S Plumber, DM Barry, D Binns, C Wichaidit, M Grzemska, S Earnest, EJ Goldsmith, O Cleaver, MH Cobb
    Proceedings of the National Academy of Sciences of the United States of America, 2022-07-22;119(30):e2203743119.  2022-07-22
  17. Two New Potential Therapeutic Approaches in Radiation Cystitis Derived from Mesenchymal Stem Cells: Extracellular Vesicles and Conditioned Medium
    Authors: C Helissey, N Guitard, H Théry, S Goulinet, P Mauduit, M Girleanu, AL Favier, M Drouet, C Parnot, C Chargari, S Cavallero, S François
    Biology, 2022-06-28;11(7):.  2022-06-28
  18. Development of secretome-based strategies to improve cell culture protocols in tissue engineering
    Authors: O Cases-Pere, C Blanco-Eli, J Chato-Astr, C Miranda-Fe, F Campos, PV Crespo, I Sánchez-Mo, M Alaminos, MA Martín-Pie, I Garzón
    Oncogene, 2022-06-15;12(1):10003.  2022-06-15
  19. Schizophrenia-derived hiPSC brain microvascular endothelial-like cells show impairments in angiogenesis and blood-brain barrier function
    Authors: BS Casas, G Vitória, CP Prieto, M Casas, C Chacón, M Uhrig, F Ezquer, M Ezquer, SK Rehen, V Palma
    Molecular Psychiatry, 2022-06-15;0(0):.  2022-06-15
  20. FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis
    Authors: Q Du, P Liu, C Zhang, T Liu, W Wang, C Shang, J Wu, Y Liao, Y Chen, J Huang, H Tan, Y Zhao, M Xia, J Liu, S Yao
    Cell Death & Disease, 2022-05-21;13(5):488.  2022-05-21
  21. The natural compound atraric acid suppresses androgen-regulated neo-angiogenesis of castration-resistant prostate cancer through angiopoietin 2
    Authors: M Ehsani, S Bartsch, SMM Rasa, J Dittmann, T Pungsrinon, L Neubert, SS Huettner, R Kotolloshi, K Schindler, A Ahmad, AS Mosig, L Adam, A Ori, F Neri, A Berndt, MO Grimm, A Baniahmad
    Oncogene, 2022-05-05;0(0):.  2022-05-05
  22. Implementing subtype-specific pre-clinical models of breast cancer to study pre-treatment aspirin effects
    Authors: IS Miller, S Khan, LP Shiels, S Das, AC O' Farrell, K Connor, A Lafferty, B Moran, C Isella, P Loadman, E Conroy, S Cohrs, R Schibli, RS Kerbel, WM Gallagher, E Marangoni, K Bennett, DP O' Connor, RM Dwyer, AT Byrne
    Cancer Medicine, 2022-04-17;0(0):.  2022-04-17
  23. A novel mechanism for A-to-I RNA-edited AZIN1 in promoting tumor angiogenesis in colorectal cancer
    Authors: Y Wei, H Zhang, Q Feng, S Wang, Y Shao, J Wu, G Jin, W Lin, X Peng, X Xu
    Cell Death & Disease, 2022-04-02;13(4):294.  2022-04-02
  24. SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an alphavbeta3 Integrin-Mediated Endocytosis Even in the Presence of Vaccine-Elicited Neutralizing Antibodies
    Authors: A Bugatti, F Filippini, M Bardelli, A Zani, P Chiodelli, S Messali, A Caruso, F Caccuri
    Viruses, 2022-03-29;14(4):.  2022-03-29
  25. Strategy for improving cell-mediated vascularized soft tissue formation in a hydrogen peroxide-triggered chemically-crosslinked hydrogel
    Authors: SY Wei, TH Chen, FS Kao, YJ Hsu, YC Chen
    Journal of tissue engineering, 2022-03-11;13(0):2041731422108.  2022-03-11
  26. PAI-1 is a vascular cell-specific HIF-2-dependent angiogenic factor that promotes retinal neovascularization in diabetic patients
    Authors: Y Qin, J Zhang, S Babapoor-F, B Applewhite, M Deshpande, H Megarity, M Flores-Bel, S Aparicio-D, T Ma, Y Rui, SY Tzeng, JJ Green, MV Canto-Sole, S Montaner, A Sodhi
    Science Advances, 2022-03-02;8(9):eabm1896.  2022-03-02
  27. Staphylococcus aureus beta-Toxin Exerts Anti-angiogenic Effects by Inhibiting Re-endothelialization and Neovessel Formation
    Authors: PM Tran, SS Tang, W Salgado-Pa
    Frontiers in Microbiology, 2022-02-03;13(0):840236.  2022-02-03
  28. Angiogenic content of microparticles in patients with diabetes and coronary artery disease predicts networks of endothelial dysfunction
    Authors: I Marei, O Chidiac, B Thomas, J Pasquier, S Dargham, A Robay, M Vakayil, M Jameesh, C Triggle, A Rafii, A Jayyousi, J Al Suwaidi, C Abi Khalil
    Cardiovascular Diabetology, 2022-02-02;21(1):17.  2022-02-02
  29. Secretome from Human Mesenchymal Stem Cells-Derived Endothelial Cells Promotes Wound Healing in a Type-2 Diabetes Mouse Model
    Authors: V Ormazabal, E Nova-Lampe, D Rojas, FA Zúñiga, C Escudero, P Lagos, A Moreno, Y Pavez, C Reyes, M Yáñez, M Vidal, G Cabrera-Vi, K Oporto, C Aguayo
    International Journal of Molecular Sciences, 2022-01-15;23(2):.  2022-01-15
  30. High glucose induced inflammation is inhibited by copper chelation via rescuing mitochondrial fusion protein 2 in retinal pigment epithelial cells
    Authors: M Aloysius D, KN Sulochana, SRB Devi
    Cellular Signalling, 2022-01-06;0(0):110244.  2022-01-06
  31. Lightwave-reinforced stem cells with enhanced wound healing efficacy
    Authors: YJ Kim, HR Jeon, SW Kim, YH Kim, GB Im, J Im, SH Um, SM Cho, JR Lee, HY Kim, YK Joung, DI Kim, SH Bhang
    Journal of tissue engineering, 2021-12-18;12(0):2041731421106.  2021-12-18
  32. Nano-Sized Extracellular Matrix Particles Lead to Therapeutic Improvement for Cutaneous Wound and Hindlimb Ischemia
    Authors: SS Ha, JH Kim, C Savitri, D Choi, K Park
    International Journal of Molecular Sciences, 2021-12-09;22(24):.  2021-12-09
  33. Evaluating the Effect of Lenvatinib on Sorafenib-Resistant Hepatocellular Carcinoma Cells
    Authors: T Shi, H Iwama, K Fujita, H Kobara, N Nishiyama, S Fujihara, Y Goda, H Yoneyama, A Morishita, J Tani, M Yamada, M Nakahara, K Takuma, T Masaki
    International Journal of Molecular Sciences, 2021-12-02;22(23):.  2021-12-02
  34. The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels
    Authors: SH Kim, D Kim, M Cha, SH Kim, Y Jung
    International Journal of Molecular Sciences, 2021-11-22;22(22):.  2021-11-22
  35. MMP9 secreted from mononuclear cell quality and quantity culture mediates STAT3 phosphorylation and fibroblast migration in wounds
    Authors: T Nishikai-Y, M Kado, H Hagiwara, S Fujimura, H Mizuno, R Tanaka
    Regenerative Therapy, 2021-11-04;18(0):464-471.  2021-11-04
  36. CMAHP promotes metastasis by reducing ubiquitination of Snail and inducing angiogenesis via GM-CSF overexpression in gastric cancer
    Authors: HW Huang, CY Chen, YH Huang, CT Yeh, CS Wang, CC Chang, KH Lin
    Oncogene, 2021-10-29;0(0):.  2021-10-29
  37. ELTD1 Activation Induces an Endothelial-EMT Transition to a Myofibroblast Phenotype
    Authors: H Sheldon, J Alexander, E Bridges, L Moreira, S Reilly, KH Ang, D Wang, S Lin, S Haider, AH Banham, AL Harris
    International Journal of Molecular Sciences, 2021-10-19;22(20):.  2021-10-19
  38. LTBP4 affects renal fibrosis by influencing angiogenesis and altering mitochondrial structure
    Authors: CT Su, TM Jao, Z Urban, YJ Huang, DHW See, YC Tsai, WC Lin, JW Huang
    Cell Death & Disease, 2021-10-13;12(10):943.  2021-10-13
  39. Eicosanoid regulation of debris-stimulated metastasis
    Authors: J Deng, H Yang, VM Haak, J Yang, FC Kipper, C Barksdale, SH Hwang, A Gartung, DR Bielenberg, S Subbian, KK Ho, X Ye, D Fan, Y Sun, BD Hammock, D Panigrahy
    Proceedings of the National Academy of Sciences of the United States of America, 2021-10-12;118(41):.  2021-10-12
  40. Dexamethasone enhances the efficacy of atorvastatin in inhibiting excessively inflammation-induced abnormal angiogenesis by regulating macrophages
    Authors: Z Gong, D Zhan, M Nie, X Li, C Gao, X Liu, T Xiang, J Yuan, W Jiang, J Huang, W Quan, D Wang, Y Tian, H Yuan, J Zhang, R Jiang
    Journal of Neuroinflammation, 2021-09-15;18(1):203.  2021-09-15
  41. Identification of soluble tissue-derived biomarkers from human thyroid tissue explants maintained on a microfluidic device
    Authors: A Riley, H Jones, J England, D Kuvshinov, V Green, J Greenman
    Oncology Letters, 2021-09-13;22(5):780.  2021-09-13
  42. Construction of transplantable artificial vascular tissue based on adipose tissue-derived mesenchymal stromal cells by a cell coating and cryopreservation technique
    Authors: Y Asano, D Okano, M Matsusaki, T Watabe, Y Yoshimatsu, M Akashi, H Shimoda
    Scientific Reports, 2021-09-09;11(1):17989.  2021-09-09
  43. CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance
    Authors: S Ma, LS Mangala, W Hu, E Bayaktar, A Yokoi, W Hu, S Pradeep, S Lee, PD Piehowski, A Villar-Pra, SY Wu, MH McGuire, OD Lara, C Rodriguez-, CJ LaFargue, NB Jennings, KD Rodland, T Liu, V Kundra, PT Ram, S Ramakrishn, G Lopez-Bere, RL Coleman, AK Sood
    Cell Reports, 2021-08-17;36(7):109549.  2021-08-17
  44. Keratin-Alginate Sponges Support Healing of Partial-Thickness Burns
    Authors: ZK Moay, LTH Nguyen, P Hartrianti, DP Lunny, D Leavesley, YO Kok, SJ Chong, AWC Chua, SI Tee, KW Ng
    International Journal of Molecular Sciences, 2021-08-10;22(16):.  2021-08-10
  45. Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor
    Authors: CY Hwang, SJ Yu, JK Won, SM Park, H Noh, S Lee, EJ Cho, JH Lee, KB Lee, YJ Kim, KS Suh, JH Yoon, KH Cho
    Cancer Gene Therapy, 2021-08-06;0(0):.  2021-08-06
  46. SARS-CoV-2 Infection Remodels the Phenotype and Promotes Angiogenesis of Primary Human Lung Endothelial Cells
    Authors: F Caccuri, A Bugatti, A Zani, A De Palma, D Di Silvest, E Manocha, F Filippini, S Messali, P Chiodelli, G Campisi, S Fiorentini, F Facchetti, P Mauri, A Caruso
    Microorganisms, 2021-07-03;9(7):.  2021-07-03
  47. Serum-Derived Small Extracellular Vesicles From Diabetic Mice Impair Angiogenic Property of Microvascular Endothelial Cells: Role of EZH2
    Authors: Z Cheng, V Naga Srika, MM Truongcao, M Cimini, G Huang, C Wang, C Benedict, C Gonzalez, V Mallaredy, DA Goukassian, SK Verma, R Kishore
    Journal of the American Heart Association, 2021-05-14;10(10):e019755.  2021-05-14
  48. Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes
    Authors: E Nassar, N Hassan, EA El-Ghonaim, H Hassan, MS Abdullah, TV Rottke, L Kiesel, B Greve, SA Ibrahim, M Götte
    Cancers, 2021-05-12;13(10):.  2021-05-12
  49. Low RECK Expression Is Part of the Cervical Carcinogenesis Mechanisms
    Authors: S Herbster, M Trombetta-, PT de Souza-S, A Paladino, CRF Silveira, MC Sogayar, LL Villa, AP Lepique, E Boccardo
    Cancers, 2021-05-06;13(9):.  2021-05-06
  50. Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles
    Authors: AC Andrade, M Wolf, HM Binder, FG Gomes, F Manstein, P Ebner-Peki, R Poupardin, R Zweigerdt, K Schallmose, D Strunk
    International Journal of Molecular Sciences, 2021-04-09;22(8):.  2021-04-09
  51. Efficacy of chitinase-3-like protein 1 as an in�vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells
    Authors: N Komabashir, F Suehiro, M Ishii, M Nishimura
    Regenerative Therapy, 2021-03-27;18(0):38-50.  2021-03-27
  52. Syndecan-1 Overexpressing Mesothelioma Cells Inhibit Proliferation, Wound Healing, and Tube Formation of Endothelial Cells
    Authors: J Javadi, G Heidari-Ha, A Schmalzl, T Szatmári, M Metintas, P Aspenström, A Hjerpe, K Dobra
    Cancers, 2021-02-06;13(4):.  2021-02-06
  53. The ER stress response mediator ERO1 triggers cancer metastasis by favoring the angiogenic switch in hypoxic conditions
    Authors: E Varone, A Decio, A Chernoruds, L Minoli, L Brunelli, F Ioli, A Piotti, R Pastorelli, M Fratelli, M Gobbi, R Giavazzi, E Zito
    Oncogene, 2021-02-02;0(0):.  2021-02-02
  54. RUNX3 Transcript Variants Have Distinct Roles in Ovarian Carcinoma and Differently Influence Platinum Sensitivity and Angiogenesis
    Authors: K Heinze, M Hölzer, M Ungelenk, M Gerth, J Thomale, R Heller, CR Morden, KJ McManus, AS Mosig, M Dürst, IB Runnebaum, N Häfner
    Cancers, 2021-01-26;13(3):.  2021-01-26
  55. Ricinus communis Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness
    Authors: R Mabasa, K Malemela, K Serala, M Kgakishe, T Matsebatle, M Mokgotho, V Mbazima
    Integrative cancer therapies, 2021-01-01;20(0):1534735420977.  2021-01-01
  56. In vitro modeling of solid tumor interactions with perfused blood vessels
    Authors: TJ Kwak, E Lee
    Sci Rep, 2020-11-19;10(1):20142.  2020-11-19
  57. Crosstalk between microglia and patient-derived glioblastoma cells inhibit invasion in a three-dimensional gelatin hydrogel model
    Authors: JE Chen, J Lumibao, S Leary, JN Sarkaria, AJ Steelman, HR Gaskins, BAC Harley
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  58. Human mesenchymal stromal/stem cells recruit resident pericytes and induce blood vessels maturation to repair experimental spinal cord injury in rats
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  153. Aberrant chloride intracellular channel 4 expression contributes to endothelial dysfunction in pulmonary arterial hypertension.
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    FASEB J, 2013-11-13;28(2):740-51.  2013-11-13
  158. Functional and molecular characterization of ex vivo cultured epiretinal membrane cells from human proliferative diabetic retinopathy.
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    Biomed Res Int, 2013-10-01;2013(0):492376.  2013-10-01
  159. A novel serum protein signature associated with resistance to epidermal growth factor receptor tyrosine kinase inhibitors in head and neck squamous cell carcinoma.
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    Eur J Cancer, 2013-04-10;49(0):2512-21.  2013-04-10
  160. Inhibition of TGF-β signaling by the fungal lactones (S)-curvularin, dehydrocurvularin, oxacyclododecindione and galiellalactone.
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  164. Cyclin-dependent kinase 8 mediates chemotherapy-induced tumor-promoting paracrine activities.
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  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  2. What are the associated pathways each factor is involved in?

    • We do not have specific angiogenesis-related categorization of the target for this array, but we do have some informative material available in our website:

  3. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

  4. Which gene of the VEGF family is detected on E19 and E20

    • The VEGF spots are detecting VEGF-A.

  5. Does the Human Angiogenesis Proteome Profiler Array cross-react with other species like equines?

    • We have not tested this kit with equine samples. However, when looking at the cross reactivity with our Proteome Profiler Arrays, there are a few things to keep in mind. When the arrays are being designed, we look for the best antibodies that will work for the species stated. This does not mean there will not be any cross reactivity between species but it does suggest that the assay would not perform equally between species. While it is possible that some of the antibodies may detect the equine protein, different antibodies cross-react at different levels.

      It is also important to note that the Proteome Profiler Arrays are designed to be used as a screening tool. This may be a good place to start if you are trying determine what may be having an increase or a decrease in signal. We recommend running one untreated sample and comparing them to your treated samples. Even though you may not have the exact same cross reactivity with each analyte, you may be able to detect the increase or decrease from your samples.

      We have searched for citations that may have referenced using this kit with equine samples and were able to find 1. Here is the link to it:

View all Proteome Profiler Antibody Array FAQs

Reviews for Proteome Profiler Human Angiogenesis Array Kit

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Proteome Profiler Human Angiogenesis Array Kit
By Anonymous on 10/31/2023

We ordered the Angiogenesis Array Kit for both mice and humans, and it is highly sensitive for both solid tumor and serum samples.

Proteome Profiler Human Angiogenesis Array Kit
By Gabriele Strusi on 11/21/2022

HepG2 lysate concentrated, DMEM FBS free, 300 ug loaded.

Proteome Profiler Human Angiogenesis Array Kit
By Anonymous on 02/07/2019

Proteome Profiler Human Angiogenesis Array Kit
By Anonymous on 02/06/2019

Worked well to look at THP-1 angiogenesis factors.

Proteome Profiler Human Angiogenesis Array Kit
By Anonymous on 10/22/2018

It worked well in cell culture supernatants using 125 ug of total protein per membrane.

Proteome Profiler Human Angiogenesis Array Kit
By Anonymous on 08/08/2018

Proteome Profiler Human Angiogenesis Array Kit
By Anonymous on 05/17/2018

Used it on Conditioned medium collected from Breast Cancer Cells treated with Angiotensin II to look into its effect on Angiogenesis. Angiogenesis array worked really well and very easy to use. It provided some new promising gene targets which we did not know before.

Proteome Profiler Human Angiogenesis Array Kit
By Leslie Priddy on 04/01/2018

Proteome Profiler Human Angiogenesis Array Kit
By Adam Guess on 10/30/2017

Proteome Profiler Human Angiogenesis Array Kit
By Anonymous on 12/06/2016

Human Angiogenesis Antibody Array was used to evaluate mesenchymal stem cells secretion profiles when seeded in various extracellular matrices. Great tool that ganarates lots of data!