Human IL-6 Antibody

(22 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL-6
    Pro29-Met212
    Accession # P05231
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 15-45 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IL‑6 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with PHA. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
IL‑6 in Human PBMCs. IL‑6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND50 is typically 15-45 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6-9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).

  • References:
    1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
    2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
    3. Jones, S.A. (2005) J. Immunol. 175:3468.
    4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
    5. Hirano, T. et al. (1986) Nature 324:73.
    6. Alberti, L. et al. (2005) Cancer Res. 65:2.
    7. Kestler, D.P. et al. (1995) Blood 86:4559.
    8. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
    9. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
    10. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.
    11. Murakami, M. et al. (1993) Science 260:1808.
    12. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
    13. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125. 
  • Long Name:
    Interleukin 6
  • Entrez Gene IDs:
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
  • Alternate Names:
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ
    Authors: M Sameni, D Cavallo-Me, OE Franco, A Chalasani, K Ji, N Aggarwal, A Anbalagan, X Chen, RR Mattingly, SW Hayward, BF Sloane
    Breast Cancer Res., 2017;19(1):56.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. SorLA in interleukin-6 signaling and turnover
    Authors: JV Larsen, CM Petersen
    Mol. Cell. Biol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
    Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
    Nat Cell Biol, 2015;17(9):1193-204.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration.
    Authors: Osuala K, Sameni M, Shah S, Aggarwal N, Simonait M, Franco O, Hong Y, Hayward S, Behbod F, Mattingly R, Sloane B
    BMC Cancer, 2015;15(0):584.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014;2014(0):593562.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  6. Digital microfluidic assay for protein detection.
    Authors: Mok J, Mindrinos M, Davis R, Javanmard M
    Proc Natl Acad Sci U S A, 2014;111(6):2110-5.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Microfluidic Assay
  7. Inhibition of IL-6 expression in LNCaP prostate cancer cells by a combination of atorvastatin and celecoxib.
    Authors: Wang H, Cui X, Goodin S, Ding N, Van Doren J, Du Z, Huang M, Liu Y, Cheng X, Dipaola R, Conney A, Zheng X
    Oncol Rep, 2014;31(2):835-41.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  9. p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
    Authors: Freund A, Patil CK, Campisi J
    EMBO J., 2011;30(8):1536-48.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
    Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
    J. Biol. Chem., 2011;286(39):34271-85.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  11. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009;69(1):329-37.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha.
    Authors: Schell C, Albrecht M, Mayer C, Schwarzer JU, Frungieri MB, Mayerhofer A
    Endocrinology, 2008;149(4):1678-86.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
    Authors: Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(38):14692-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Oncogene-induced senescence relayed by an interleukin-dependent inflammatory network.
    Authors: Kuilman T, Michaloglou C, Vredeveld LC, Douma S, van Doorn R, Desmet CJ, Aarden LA, Mooi WJ, Peeper DS
    Cell, 2008;133(6):1019-31.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
    Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
    Blood, 2007;109(11):4856-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  16. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
    Authors: Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de La Pintiere CT, Genetet N, Patard JJ, Catros-Quemener V
    Clin. Exp. Immunol., 2006;146(3):518-23.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  18. Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
    Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
    Exp. Cell Res., 2006;312(10):1909-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  19. Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma.
    Authors: Feldmann G, Nischalke HD, Nattermann J, Banas B, Berg T, Teschendorf C, Schmiegel W, Duhrsen U, Halangk J, Iwan A, Sauerbruch T, Caselmann WH, Spengler U
    Clin. Cancer Res., 2006;12(15):4491-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  20. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004;80(2):231-5.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  21. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
    Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
    J. Virol., 2003;77(3):1727-37.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Neut
  22. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 22 Citations

FAQs

  1. What band size does AF-206-NA detect in Western blot?
    • AF-206-NA detects a 20-22 kDa band in lysate in Western blot.

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