Human IL-6 Antibody

Catalog # Availability Size / Price Qty
AF-206-NA
AF-206-SP
Human IL-6 Antibody in Western Blot
3 Images
Product Details
Citations (28)
FAQs
Supplemental Products
Reviews (2)

Human IL-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-6
Pro29-Met212
Accession # P05231
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 15-45 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human IL‑6 by Western Blot. View Larger

Detection of Human IL‑6 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with PHA. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL‑6 in Human PBMCs. View Larger

IL‑6 in Human PBMCs. IL‑6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND50 is typically 15-45 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Product Datasheets

Citations for Human IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
    Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
    Breast Cancer Res., 2019;21(1):31.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
    Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Applications: Affinity Assay
  3. Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice
    Authors: Y Yin, H Hao, Y Cheng, L Zang, J Liu, J Gao, J Xue, Z Xie, Q Zhang, W Han, Y Mu
    Cell Death Dis, 2018;9(7):760.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes
    Authors: A Klaesson, K Grannas, T Ebai, J Heldin, B Koos, M Leino, D Raykova, J Oelrich, L Arngården, O Söderberg, U Landegren
    Sci Rep, 2018;8(1):5400.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Proximity Ligation Assay (PLA)
  5. Adipose Stromal Cells from Visceral and Subcutaneous Fat Facilitate Migration of Ovarian Cancer Cells via IL-6/JAK2/STAT3 Pathway
    Authors: B Kim, HS Kim, S Kim, G Haegeman, BK Tsang, DN Dhanasekar, YS Song
    Cancer Res Treat, 2017;49(2):338-349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ
    Authors: M Sameni, D Cavallo-Me, OE Franco, A Chalasani, K Ji, N Aggarwal, A Anbalagan, X Chen, RR Mattingly, SW Hayward, BF Sloane
    Breast Cancer Res., 2017;19(1):56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. SorLA in interleukin-6 signaling and turnover
    Authors: JV Larsen, CM Petersen
    Mol. Cell. Biol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
    Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
    Nat Cell Biol, 2015;17(9):1193-204.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration.
    Authors: Osuala K, Sameni M, Shah S, Aggarwal N, Simonait M, Franco O, Hong Y, Hayward S, Behbod F, Mattingly R, Sloane B
    BMC Cancer, 2015;15(0):584.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Inhibition of IL-6 expression in LNCaP prostate cancer cells by a combination of atorvastatin and celecoxib.
    Authors: Wang H, Cui X, Goodin S, Ding N, Van Doren J, Du Z, Huang M, Liu Y, Cheng X, Dipaola R, Conney A, Zheng X
    Oncol Rep, 2014;31(2):835-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014;2014(0):593562.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  12. Digital microfluidic assay for protein detection.
    Authors: Mok J, Mindrinos M, Davis R, Javanmard M
    Proc Natl Acad Sci U S A, 2014;111(6):2110-5.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Microfluidic Assay
  13. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  14. Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
    Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
    J. Biol. Chem., 2011;286(39):34271-85.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  15. p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
    Authors: Freund A, Patil CK, Campisi J
    EMBO J., 2011;30(8):1536-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009;69(1):329-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha.
    Authors: Schell C, Albrecht M, Mayer C, Schwarzer JU, Frungieri MB, Mayerhofer A
    Endocrinology, 2008;149(4):1678-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Oncogene-induced senescence relayed by an interleukin-dependent inflammatory network.
    Authors: Kuilman T, Michaloglou C, Vredeveld LC, Douma S, van Doorn R, Desmet CJ, Aarden LA, Mooi WJ, Peeper DS
    Cell, 2008;133(6):1019-31.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
    Authors: Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(38):14692-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
    Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
    Blood, 2007;109(11):4856-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma.
    Authors: Feldmann G, Nischalke HD, Nattermann J, Banas B, Berg T, Teschendorf C, Schmiegel W, Duhrsen U, Halangk J, Iwan A, Sauerbruch T, Caselmann WH, Spengler U
    Clin. Cancer Res., 2006;12(15):4491-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
    Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
    Exp. Cell Res., 2006;312(10):1909-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
    Authors: Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de La Pintiere CT, Genetet N, Patard JJ, Catros-Quemener V
    Clin. Exp. Immunol., 2006;146(3):518-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  25. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004;80(2):231-5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  26. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
    Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
    J. Virol., 2003;77(3):1727-37.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production.
    Authors: Gringhuis S, Kaptein T, Wevers B, van der Vlist M, Klaver E, van Die I, Vriend L, de Jong M, Geijtenbeek T
    Nat Commun, 0;5(0):5074.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

  1. What band size does AF-206-NA detect in Western blot?
    • AF-206-NA detects a 20-22 kDa band in lysate in Western blot.

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Reviews for Human IL-6 Antibody

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Human IL-6 Antibody
By Anonymous on 11/01/2018
Application: MSD Assay Sample Tested: Vitreous Humor Species: Cynomolgus Monkey

After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC). Paired with biotinylated IL-6 antibody (MAB206) as a capture reagent. A standard curve with recombinant human IL-6 from (Cat# 206-IL/CF) is shown (1.6-25,000 pg/ml).


Human IL-6 Antibody
By Anonymous on 05/11/2018
Application: Cell Depl Sample Tested: Pancreatic cancer cells Species: Human