Human TNF-alpha Antibody

Catalog # Availability Size / Price Qty
AF-210-NA
AF-210-SP
Cytotoxicity Induced by TNF‑ alpha  and Neutralization by Human TNF-alpha  Antibody.
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Product Details
Citations (23)
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Reviews (3)

Human TNF-alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF‑ alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant equine TNF-alpha is observed and approximately 20% cross‑reactivity with recombinant cotton rat TNF‑ alpha, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha is observed and less than 5% cross‑reactivity with recombinant porcine TNF‑ alpha and recombinant bovine TNF‑ alpha  is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TNF-alpha
Val77-Leu233
Accession # P01375
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.01-0.06 μg/ml in the presence of 1.5 ng/mL Recombinant Human TNF‑ alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Cytotoxicity Induced by TNF‑ alpha  and Neutralization by Human TNF-alpha  Antibody. View Larger

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF-alpha Antibody.

Recombinant Human TNF‑ alpha (Catalog # 210-TA) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA). The ND50 is typically 0.01‑0.06 μg/mL.

Immunocytochemistry TNF‑ alpha   in Human PBMCs. View Larger

TNF‑ alpha in Human PBMCs. TNF‑ alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-210-NA) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types. Human TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%‑92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-alpha regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-alpha is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Product Datasheets

Citations for Human TNF-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Intravascular heavy chain-modification of hyaluronan during endotoxic shock
    Authors: K Ni, A Gill, D Cao, K Koike, KS Schweitzer, S Garantziot, I Petrache
    Biochem Biophys Rep, 2019;17(0):114-121.
    Species: Human
    Sample Types: Cell Culture Supernate
    Applications: Western Blot
  2. Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer
    Authors: M Kim, S Pyo, CH Kang, CO Lee, HK Lee, SU Choi, CH Park
    PLoS ONE, 2018;13(6):e0198347.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
    Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Applications: Affinity Assay
  4. Detection of aryl hydrocarbon receptor agonists in human samples
    Authors: V Rothhammer, DM Borucki, JE Kenison, P Hewson, Z Wang, R Bakshi, DH Sherr, FJ Quintana
    Sci Rep, 2018;8(1):4970.
    Species: Human
    Sample Types: Serum
    Applications: Neutralization
  5. Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients
    PLoS ONE, 2016;11(9):e0162908.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Promising Role of Toll-Like Receptor 8 Agonist in Concert with Prostratin for Activation of Silent HIV
    Authors: M A Rochat
    J. Virol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
    Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
    Proc Natl Acad Sci U S A, 2014;111(52):E5688-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  9. Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein.
    Authors: Hasegawa H, Thomas HJ, Schooley K, Born TL
    Cytokine, 2011;53(1):74-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
    Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
    J. Immunol., 2011;186(7):4175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
    Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
    Blood, 2010;116(12):2061-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  12. Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.
    Authors: Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW
    J. Exp. Med., 2009;206(9):1883-97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
    Authors: Navenot JM, Fujii N, Peiper SC
    Mol. Pharmacol., 2009;75(5):1074-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
    Authors: Lee SY, Cherla RP, Tesh VL
    Infect. Immun., 2007;75(3):1291-302.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  16. Sepsis and pathophysiology of anthrax in a nonhuman primate model.
    Authors: Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S
    Am. J. Pathol., 2006;169(2):433-44.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Plasma
    Applications: ELISA Development
  17. Spatial compartmentalization of tumor necrosis factor (TNF) receptor 1-dependent signaling pathways in human airway smooth muscle cells. Lipid rafts are essential for TNF-alpha-mediated activation of RhoA but dispensable for the activation of the NF-kappaB and MAPK pathways.
    Authors: Hunter I, Nixon GF
    J. Biol. Chem., 2006;281(45):34705-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104.
    Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D
    J. Leukoc. Biol., 2005;78(2):359-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004;80(2):231-5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
    Authors: Andersson By U, Tani E, Andersson U
    J. Pediatr. Hematol. Oncol., 2004;26(11):706-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
    Authors: Park JS, Kim YS, Jee YK, Myong NH, Lee KY
    Scand. J. Immunol., 2003;57(5):463-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells.
    Authors: Edgeworth JD, Spencer J, Phalipon A, Griffin GE, Sansonetti PJ
    Eur. J. Immunol., 2002;32(5):1464-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD
    J Leukoc Biol, 0;91(2):299-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human TNF-alpha Antibody

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Human TNF-alpha Antibody
By Anonymous on 04/13/2018
Application: IHC Sample Tested: Adult brain Species: Human

Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).


Human TNF-alpha Antibody
By Anonymous on 01/18/2018
Application: ELISA Sample Tested: Heparin Plasma,EDTA Plasma,Citrate Plasma Species: Human

Human TNF-alpha Antibody
By Anonymous on 11/16/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used with MAB610 to build an ELISA to measure TNFa. This antibody was used as the detection while MAB610 was used as the capture.