Human TNF-alpha Antibody
Human TNF-alpha Antibody Summary
Val77-Leu233
Accession # P01375
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA). The ND50 is typically 0.01-0.06 µg/mL.
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TNF‑ alpha in Human PBMCs. TNF-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF-alpha
Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF- is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.
Product Datasheets
Citations for Human TNF-alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Intravascular heavy chain-modification of hyaluronan during endotoxic shock
Authors: K Ni, A Gill, D Cao, K Koike, KS Schweitzer, S Garantziot, I Petrache
Biochem Biophys Rep, 2019;17(0):114-121.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer
Authors: M Kim, S Pyo, CH Kang, CO Lee, HK Lee, SU Choi, CH Park
PLoS ONE, 2018;13(6):e0198347.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Applications: Affinity Assay -
Detection of aryl hydrocarbon receptor agonists in human samples
Authors: V Rothhammer, DM Borucki, JE Kenison, P Hewson, Z Wang, R Bakshi, DH Sherr, FJ Quintana
Sci Rep, 2018;8(1):4970.
Species: Human
Sample Types: Serum
Applications: Neutralization -
Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients
PLoS ONE, 2016;11(9):e0162908.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Promising Role of Toll-Like Receptor 8 Agonist in Concert with Prostratin for Activation of Silent HIV
Authors: M A Rochat
J. Virol, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
Proc Natl Acad Sci U S A, 2014;111(52):E5688-96.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
Cytokine, 2013;64(3):660-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein.
Authors: Hasegawa H, Thomas HJ, Schooley K, Born TL
Cytokine, 2011;53(1):74-83.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
J. Immunol., 2011;186(7):4175-82.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
Blood, 2010;116(12):2061-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.
Authors: Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW
J. Exp. Med., 2009;206(9):1883-97.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
Authors: Navenot JM, Fujii N, Peiper SC
Mol. Pharmacol., 2009;75(5):1074-83.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Authors: Skibinski G, Elborn JS, Ennis M
Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
Authors: Lee SY, Cherla RP, Tesh VL
Infect. Immun., 2007;75(3):1291-302.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Sepsis and pathophysiology of anthrax in a nonhuman primate model.
Authors: Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S
Am. J. Pathol., 2006;169(2):433-44.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Plasma
Applications: ELISA Development -
Spatial compartmentalization of tumor necrosis factor (TNF) receptor 1-dependent signaling pathways in human airway smooth muscle cells. Lipid rafts are essential for TNF-alpha-mediated activation of RhoA but dispensable for the activation of the NF-kappaB and MAPK pathways.
Authors: Hunter I, Nixon GF
J. Biol. Chem., 2006;281(45):34705-15.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104.
Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D
J. Leukoc. Biol., 2005;78(2):359-71.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
Photochem. Photobiol., 2004;80(2):231-5.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
Authors: Andersson By U, Tani E, Andersson U
J. Pediatr. Hematol. Oncol., 2004;26(11):706-11.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
Authors: Park JS, 107290, Kim YS, Jee YK, Myong NH, Lee KY
Active immunotherapy against pathogenic Cis pT231-tau suppresses neurodegeneration in traumatic brain injury mouse models, 2003;57(5):463-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells.
Authors: Edgeworth JD, Spencer J, Phalipon A, Griffin GE, Sansonetti PJ
Eur. J. Immunol., 2002;32(5):1464-71.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD
J Leukoc Biol, 0;91(2):299-309.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).
This antibody was used with MAB610 to build an ELISA to measure TNFa. This antibody was used as the detection while MAB610 was used as the capture.
