Mouse IL-6 Antibody

Catalog # Availability Size / Price Qty
AF-406-NA
AF-406-SP
Mouse IL-6 Antibody in Western Blot
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Product Details
Citations (26)
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Mouse IL-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-6 in direct ELISAs and Western blots.  In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL‑6 and recombinant cotton rat IL-6 is observed and less than 15% cross-reactivity with recombinant human IL-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-6
Phe25-Thr211
Accession # P08505
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed mouse splenocytes treated with PMA and ionomycin
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of  Mouse IL‑6 by Western Blot. View Larger

Detection of Mouse IL‑6 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL‑6  in Mouse Thymus. View Larger

IL‑6 in Mouse Thymus. IL‑6 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody. Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA). The ND50 is typically
0.01-0.03 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Product Datasheets

Citations for Mouse IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Glucose Ingestion Inhibits Endurance Exercise-Induced IL-6 Producing Macrophage Infiltration in Mice Muscle
    Authors: T Tominaga, S Ma, K Saitou, K Suzuki
    Nutrients, 2019;11(7):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation
    Authors: OJ Sul, M Rajasekara, HJ Park, JH Suh, HS Choi
    Oxid Med Cell Longev, 2019;2019(0):6018180.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis
    Authors: J Jhun, SH Lee, SY Kim, J Ryu, JY Kwon, HS Na, K Jung, SJ Moon, ML Cho, JK Min
    J Transl Med, 2019;17(1):84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Evidence for acute contraction-induced myokine secretion by C2C12 myotubes
    Authors: Y Furuichi, Y Manabe, M Takagi, M Aoki, NL Fujii
    PLoS ONE, 2018;13(10):e0206146.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. 3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation
    Authors: LL Xu, T Liu, L Wang, L Li, YF Wu, CC Li, B Di, QD You, ZY Jiang
    Oxid Med Cell Longev, 2018;2018(0):3271617.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus
    Authors: WJ Su, T Zhang, CL Jiang, W Wang
    Front Cell Neurosci, 2018;12(0):412.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. BATF regulates collagen-induced arthritis by regulating T helper cell differentiation
    Authors: SH Park, J Rhee, SK Kim, JA Kang, JS Kwak, YO Son, WS Choi, SG Park, JS Chun
    Arthritis Res. Ther., 2018;20(1):161.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice
    Authors: C Dempsey
    Brain Behav. Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  9. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
    Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
    Sci Rep, 2016;6(0):19404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A.
    Authors: Rudraraju R, Jones B, Surman S, Sealy R, Thomas P, Hurwitz J
    PLoS ONE, 2014;9(1):e86554.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
    Authors: Uto-Konomi A, Miyauchi K, Ozaki N
    PLoS ONE, 2012;7(7):e40343.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
    Authors: Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S
    J. Clin. Invest., 2011;121(5):1692-708.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
    Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
    J. Clin. Invest., 2011;121(10):3846-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Foxa1 and Foxa2 regulate bile duct development in mice.
    Authors: Li Z, White P, Tuteja G, Rubins N, Sackett S, Kaestner KH
    J. Clin. Invest., 2009;119(6):1537-45.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway.
    Authors: Weng TI, Wu HY, Lin PY, Liu SH
    Infect. Immun., 2009;77(8):3312-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. The critical role of invading peripheral macrophage-derived interleukin-6 in vincristine-induced mechanical allodynia in mice.
    Authors: Kiguchi N, Maeda T, Kobayashi Y, Kondo T, Ozaki M, Kishioka S
    Eur. J. Pharmacol., 2008;592(1):87-92.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.
    Authors: Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y
    J. Immunol., 2008;180(6):4227-34.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.
    Authors: Tamagawa E, Bai N, Morimoto K, Gray C, Mui T, Yatera K, Zhang X, Xing L, Li Y, Laher I, Sin DD, Man SF, van Eeden SF
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;295(1):L79-85.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  20. Mechanisms of interleukin-6 synthesis and release induced by interleukin-1 and cell depolarisation in neurones.
    Authors: Tsakiri N, Kimber I, Rothwell NJ, Pinteaux E
    Mol. Cell. Neurosci., 2007;37(1):110-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. High pressure promotes monocyte adhesion to the vascular wall.
    Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
    Circ. Res., 2007;100(8):1226-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
    Authors: Kurooka M, Kaneda Y
    Cancer Res., 2007;67(1):227-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Interleukin-6 as a mechanism for the adverse effects of social stress on acute Theiler's virus infection.
    Authors: Meagher MW, Johnson RR, Young EE, Vichaya EG, Lunt S, Hardin EA, Connor MA, Welsh CJ
    Brain Behav. Immun., 2007;21(8):1083-95.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  24. The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors.
    Authors: Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS
    J. Invest. Dermatol., 2005;125(5):1053-62.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  25. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  26. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000;95(6):1957-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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