Rat IL-6 Antibody

Catalog #: AF506
28 Citations Datasheet / COA / SDS

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AF506
AF506-SP

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Citations (28)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IL-6 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat and mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant cotton rat IL‑6 is observed, and less than 1% cross-reactivity with recombinant human IL-6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat IL-6
Phe25-Thr211
Accession # P20607

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND₅₀) is typically <0.5 µg/mL in the presence of 0.6 ng/mL Recombinant Rat IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat IL‑6 by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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IL‑6 in NR8383 Rat Cell Line. IL‑6 was detected in immersion fixed NR8383 rat alveolar macrophage cell line using Goat Anti-Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cyoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization

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Cell Proliferation Induced by IL‑6 and Neutralization by Rat IL‑6 Antibody. Recombinant Rat IL-6 (Catalog # 506-RL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-6 (0.6 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506). The ND₅₀ is typically <0.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature rat IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Rat IL-6 shares 39% and 85% aa sequence identity with human and mouse IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Alternate Names

B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Rat IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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Effects of Energy Drink Acute Assumption in Gastrointestinal Tract of Rats
Authors: M Nasi, A De Gaetano, G Carnevale, L Bertoni, V Selleri, G Zanini, A Pisciotta, S Caramaschi, L Reggiani B, A Farinetti, A Cossarizza, M Pinti, A Manenti, AV Mattioli
Nutrients, 2022;14(9):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Protective Effects of Growth Differentiation Factor-6 on the Intervertebral Disc: An In Vitro and In Vivo Study
Authors: K Miyazaki, S Miyazaki, T Yurube, Y Takeoka, Y Kanda, Z Zhang, Y Kakiuchi, R Tsujimoto, H Ohnishi, T Matsuo, M Ryu, R Kuroda, K Kakutani
Cells, 2022;11(7):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy
Authors: M Jiang, H Xie, C Zhang, T Wang, H Tian, L Lu, JY Xu, GT Xu, L Liu, J Zhang
Journal of Cellular and Molecular Medicine, 2022;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Neuronal ApoE4 stimulates C/EBPbeta activation, promoting Alzheimer's disease pathology in a mouse model
Authors: ZH Wang, Y Xia, Z Wu, SS Kang, JC Zhang, P Liu, X Liu, W Song, V Huin, CM Dhaenens, SP Yu, XC Wang, K Ye
Progress in neurobiology, 2021;209(0):102212.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
ApoE4 activates C/EBP&beta/&delta-secretase with 27-hydroxycholesterol, driving the pathogenesis of Alzheimer's disease
Authors: ZH Wang, Y Xia, P Liu, X Liu, L Edgington-, K Lei, SP Yu, XC Wang, K Ye
Progress in neurobiology, 2021;0(0):102032.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation
Authors: M Yamamoto, M Kim, H Imai, Y Itakura, G Ohtsuki
Cell Rep, 2019;28(11):2923-2938.e8.
Species: Rat
Sample Types: Tissue Culture Supernates
Applications: Western Blot
Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury
Authors: S Zilberman-, R Abu-Hamad, R Zarura, M Sova, Y Hachmo, M Stark, S Neuman, S Slavin, S Efrati
PLoS ONE, 2019;14(9):e0222354.
Species: Rat
Sample Types: Whole Tissue
Applications: Fuorescense Microscopy
Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates Î´-Secretase by Upregulating C/EBP? in Alzheimer's Disease
Authors: ZH Wang, J Xiang, X Liu, SP Yu, FP Manfredsso, IM Sandoval, S Wu, JZ Wang, K Ye
Cell Rep, 2019;28(3):655-669.e5.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization
Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model
Authors: SA Chong, S Balosso, C Vandenplas, G Szczesny, E Hanon, K Claes, X Van Damme, B Danis, J Van Eyll, C Wolff, A Vezzani, RM Kaminski, I Niespodzia
Neurotherapeutics, 2018;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization
Early CALP2 expression and microglial activation are potential inducers of spinal IL-6 upregulation and bilateral pain following motor nerve injury
Authors: SX Chen, SK Wang, PW Yao, GJ Liao, XD Na, YY Li, WA Zeng, XG Liu, Y Zang
J. Neurochem., 2018;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr

Upregulation of CD81 in trophoblasts induces an imbalance of Treg/Th17 cells by promoting IL-6 expression in preeclampsia
Authors: H Ding, Y Dai, Y Lei, Z Wang, D Liu, R Li, L Shen, N Gu, M Zheng, X Zhu, G Zhao, Y Hu
Cell. Mol. Immunol., 2018;0(0):.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization

Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection
Authors: HC Lai, CH Lu, CS Wong, BF Lin, SM Chan, CY Kuo, ZF Wu
J Chin Med Assoc, 2018;0(0):.
Species: Rat
Sample Types: Whole Tissue

Mesenchymal stem cells and cell-derived extracellular vesicles protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-? oligomers
Authors: MA Godoy, LM Saraiva, LRP Carvalho, A Vasconcelo, HJV Beiral, AB Ramos, LRP Silva, RB Leal, VHS Monteiro, CV Braga, CA Araujo-Sil, LC Sinis, VB Santos, THK Brunswick, CL Alcantara, APCA Lima, NLD Cunha E Si, A Galina, A Vieyra, FG De Felice, R Mendez-Ote, ST Ferreira
J. Biol. Chem., 2017;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Repeated water avoidance stress induces visceral hypersensitivity; role of IL-1, IL-6 and peripheral corticotropin-releasing factor
Authors: T Nozu, S Miyagishi, R Nozu, K Takakusaki, T Okumura
J. Gastroenterol. Hepatol, 2017;0(0):.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Mu-opioid receptor and delta-opioid receptor differentially regulate microglial inflammatory response to control proopiomelanocortin neuronal apoptosis in the hypothalamus: effects of neonatal alcohol
Authors: P Shrivastav, MA Cabrera, LG Chastain, NI Boyadjieva, S Jabbar, T Franklin, DK Sarkar
J Neuroinflammation, 2017;14(1):83.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Mechanisms involved in dual vasopressin/apelin neuron dysfunction during aging.
Authors: Sauvant J, Delpech J, Palin K, De Mota N, Dudit J, Aubert A, Orcel H, Roux P, Laye S, Moos F, Llorens-Cortes C, Nadjar A
PLoS ONE, 2014;9(2):e87421.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization

Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K
J Neurosci, 2014;34(6):2231-43.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Sublytic C5b-9 induces IL-6 and TGF-beta1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBPbeta acetylation.
Authors: Zhang J, Li Y, Shan K, Wang L, Qiu W, Lu Y, Zhao D, Zhu G, He F, Wang Y
FASEB J, 2014;28(3):1511-25.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca(2+) signals and an IL-6 autocrine loop.
Authors: Bustamante M, Fernandez-Verdejo R, Jaimovich E, Buvinic S
Am J Physiol Endocrinol Metab, 2014;306(8):E869-82.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Fetal asphyctic preconditioning modulates the acute cytokine response thereby protecting against perinatal asphyxia in neonatal rats.
Authors: Vlassaks E, Strackx E, Vles J, Nikiforou M, Martinez-Martinez P, Kramer B, Gavilanes A
J Neuroinflammation, 2013;10(0):14.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr

Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
Authors: Furuhashi, Kazuhiro, Tsuboi, Naotake, Shimizu, Asuka, Katsuno, Takayuki, Kim, Hangsoo, Saka, Yosuke, Ozaki, Takenori, Sado, Yoshikaz, Imai, Enyu, Matsuo, Seiichi, Maruyama, Shoichi
J Am Soc Nephrol, 2013;24(4):587-603.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness).
Authors: Murase S, Terazawa E, Queme F, Ota H, Matsuda T, Hirate K, Kozaki Y, Katanosaka K, Taguchi T, Urai H, Mizumura K
J. Neurosci., 2010;30(10):3752-61.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization

Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of neural progenitor cells from adult hippocampus.
Authors: Oh J, McCloskey MA, Blong CC
J. Neurosci. Res., 2010;88(13):2798-809.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

JAK/STAT3 pathway is activated in spinal cord microglia after peripheral nerve injury and contributes to neuropathic pain development in rat.
Authors: Dominguez E, Rivat C, Pommier B, Mauborgne A, Pohl M
J. Neurochem., 2008;107(1):50-60.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization

Trauma and hemorrhage-induced acute hepatic insulin resistance: dominant role of tumor necrosis factor-alpha.
Authors: Xu J, Kim HT, Ma Y, Zhao L, Zhai L, Kokorina N, Wang P, Messina JL
Endocrinology, 2008;149(5):2369-82.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization

Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells.
Authors: Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H
Eur. J. Neurosci., 2007;25(3):649-58.
Species: Rat
Sample Types: Cell Culture Supernates
Applications: Immunodepletion

Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
Authors: Barkho BZ, Song H, Aimone JB
Stem Cells Dev., 2006;15(3):407-21.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Mechanism of IL-6-mediated cardiac dysfunction following trauma-hemorrhage.
Authors: Yang S, Hu S, Hsieh YC, Choudhry MA, Rue LW, Bland KI, Chaudry IH
J. Mol. Cell. Cardiol., 2006;40(4):570-9.
Species: Rat
Sample Types:
Applications: Neutralization