Human IL-1 beta /IL-1F2 Antibody AF-201-NA: R&D Systems

Human IL-1 beta /IL-1F2 Antibody

(21 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30%‑75% cross-reactivity with recombinant mouse IL‑1 beta, recombinant porcine IL-1 beta, recombinant rat IL-1 beta, recombinant canine IL-1 beta, recombinant equine IL-1 beta, recombinant cotton rat IL-1 beta, recombinant feline IL‑1 beta, recombinant rhesus monkey IL-1 beta, and recombinant guinea pig IL-1 beta  is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL-1 beta /IL-1F2
    Ala117-Ser269
    Accession # NP_000567
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.004-0.02 µg/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human
IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral­ization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). The ND50 is typically 0.004-0.02 µg/mL in the presence of concanavalin A (1.25 µg/mL).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus monkey and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells
    Authors: JH Jeong, J Kim, J Kim, HR Heo, JS Jeong, YJ Ryu, Y Hong, SS Han, SH Hong, SJ Lee, WJ Kim
    Tuberc Respir Dis (Seoul), 2017;80(3):247-254.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
    Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
    Autophagy, 2017;0(0):0.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  4. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
    Authors: Yong Hwan Park
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  5. 17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
    Sci Rep, 2016;6(0):37625.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1beta for Caspase-1 Cleavage.
    Authors: Edye M, Brough D, Allan S
    J Biol Chem, 2015;290(42):25374-81.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
  7. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
    Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
    Nat Med, 2015;21(3):248-55.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
    Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
    Cytokine, 2015;71(2):223-31.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  9. Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
    Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J
    J Immunol, 2015;194(11):5472-87.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.
    Authors: Kortmann J, Brubaker S, Monack D
    J Immunol, 2015;195(3):815-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  11. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
    Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
    Eur J Immunol, 2015;45(4):1092-102.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014;2014(0):593562.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  13. Complement C5a potentiates uric acid crystal-induced IL-1beta production.
    Authors: An L, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M
    Eur J Immunol, 2014;44(12):3669-79.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
    Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
    J Lipid Res, 2013;54(11):2998-3008.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  15. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
    Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J
    Nature, 2012;492(7427):123-7.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  16. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
    Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
    J. Immunol., 2011;186(7):4175-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
    Authors: Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A
    Cytokine, 2008;44(3):352-60.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  18. ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo.
    Authors: Rocks N, Paulissen G, Quesada-Calvo F, Munaut C, Gonzalez ML, Gueders M, Hacha J, Gilles C, Foidart JM, Noel A, Cataldo DD
    Cancer Res., 2008;68(22):9541-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  19. Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
    Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
    Biol. Reprod., 2007;76(6):1009-15.
    Species: Amphibian
    Sample Type: Tissue Homogenates
    Application: WB
  20. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004;80(2):231-5.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  21. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 21 Citations
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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 applications: Western Blot, Acoustic Assay on the BioScale ViBE Platform.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human IL-1 beta /IL-1F2 Antibody AF-201-NA
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Very Good
 WB Human Anonymous 04/13/2017
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Western Blot Human IL-1 beta /IL-1F2 Antibody AF-201-NA
Western Blot: Human IL-1 beta /IL-1F2 Antibody [AF-201-NA]

Summary

ApplicationWestern Blot
Sample TestedTHP-1 human acute monocytic leukemia cell line,monocyte derived macrophages
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThese Abs recognize both pro-IL1b (p31) and active, cleaved form (p17) in cell lysates and conditioned media of activated human monocyte derived macrophages and THP-1 derived macrophages. To see p17 IL-1b I used 10% tricine gel due to small size of detected protein.I use antibody at dilution 1:500
  Excellent
 Acoustic Assay on the BioScale ViBE Platform Human Anonymous 11/24/2015
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Summary

ApplicationAcoustic Assay on the BioScale ViBE Platform
Sample TestedHuman Plasma,Plasma
SpeciesHuman

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