Human IL-1 beta /IL-1F2 Antibody

Catalog # Availability Size / Price Qty
AF-201-NA
AF-201-SP
IL-1 beta /IL-1F2 in Human Tonsil Using Dual RNAscope®ISH and IHC.
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Product Details
Citations (42)
FAQs
Supplemental Products
Reviews (2)

Human IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 beta /IL-1F2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
Ala117-Ser269
Accession # NP_000567
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
TF‑1 human erythroleukemic cell line
Immunohistochemistry
1-15 µg/mL
See below
Dual RNAscope ISH-IHC
1-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 6-42 ng/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Dual RNAscope ISH-IHC Compatible IL-1 beta /IL-1F2 antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

IL-1 beta /IL-1F2 in Human Tonsil Using Dual RNAscope®ISH and IHC. IL-1 beta /IL-1F2 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 310361) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Western Blot Detection of Human IL-1 beta /IL-1F2 antibody by Western Blot. View Larger

Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry IL-1 beta /IL-1F2 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IL‑1 beta /IL‑1F2 in Human Tonsil. IL-1 beta /IL-1F2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 39 kDa (as indicated) using 1 µg/mL of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). The ND50 is typically 6-42 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

Citations for Human IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. Single cell transcriptomic landscape of diabetic foot ulcers
    Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
    Nature Communications, 2022;13(1):181.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. NLRP3 inflammasome triggers interleukin-37 release from human monocytes
    Authors: A Gritsenko, R Díaz-Pino, G Lopez-Cast
    European Journal of Immunology, 2022;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Gasdermin E permits interleukin-1 beta release in distinct sublytic and pyroptotic phases
    Authors: B Zhou, DW Abbott
    Cell Reports, 2021;35(2):108998.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8
    Authors: Q Gong, K Robinson, C Xu, PT Huynh, KHC Chong, EYJ Tan, J Zhang, ZZ Boo, DET Teo, K Lay, Y Zhang, JSY Lim, WI Goh, G Wright, FL Zhong, B Reversade, B Wu
    Nature Communications, 2021;12(1):188.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
    Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
    Redox Biology, 2021;48(0):102205.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Dot Blot
  6. Endolysosomal ion channel MCOLN2 (Mucolipin-2) promotes prostate cancer progression via IL-1beta/NF-kappaB pathway
    Authors: H Yu, M Xie, Z Meng, CY Lo, FL Chan, L Jiang, X Meng, X Yao
    British Journal of Cancer, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The Blockade of Tumoral IL1&beta-Mediated Signaling in Normal Colonic Fibroblasts Sensitizes Tumor Cells to Chemotherapy and Prevents Inflammatory CAF Activation
    Authors: NG Díaz-Marot, G Garcia-Vic, G Polcaro, M Bañuls, N Albert, A Villanueva, DG Molleví
    International Journal of Molecular Sciences, 2021;22(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Glycyrrhizin prevents SARS-CoV-2 S1 and Orf3a induced high mobility group box 1 (HMGB1) release and inhibits viral replication
    Authors: P Gowda, S Patrick, SD Joshi, RK Kumawat, E Sen
    Cytokine, 2021;142(0):155496.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Platelets Fuel the Inflammasome Activation of Innate Immune Cells
    Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
    Cell Rep, 2020;31(6):107615.
    Species: Human
    Sample Types:
    Applications: Blocking/Neutralizing
  10. Interleukin 1&beta/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy
    Authors: Y Chen, Z Yang, B Deng, D Wu, Y Quan, Z Min
    Oncol. Rep., 2020;43(3):908-918.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  11. Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin in?ammation
    Authors: Z Wang, H Zhou, H Zheng, X Zhou, G Shen, X Teng, X Liu, J Zhang, X Wei, Z Hu, F Zeng, Y Hu, J Hu, X Wang, S Chen, J Cheng, C Zhang, Y Gui, S Zou, Y Hao, Q Zhao, W Wu, Y Zhou, K Cui, N Huang, Y Wei, W Li, J Li
    Autophagy, 2020;0(0):1-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Inflammasome Sensor NLRP1 Confers Acquired Drug Resistance to Temozolomide in Human Melanoma
    Authors: Z Zhai, JM Samson, T Yamauchi, PK Vaddi, Y Matsumoto, CA Dinarello, D Ravindran, M Fujita
    Cancers (Basel), 2020;12(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration
    Authors: C De Witte, K Demeyere, S De Bruycke, B Taminiau, G Daube, R Ducatelle, E Meyer, F Haesebrouc
    Vet. Res., 2019;50(1):39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells
    Authors: R Cheng, S Billet, C Liu, S Haldar, D Choudhury, M Tripathi, M Hav, A Merchant, T Hu, H Huang, H Zhou, NA Bhowmick
    Oncogene, 2019;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1? Protein during Virion-Mediated Activation of the AIM2 Inflammasome
    Authors: S Botto, J Abraham, N Mizuno, K Pryke, B Gall, I Landais, DN Streblow, KJ Fruh, VR DeFilippis
    MBio, 2019;10(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  16. Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages
    Authors: PJ Goddard, J Sanchez-Ga, SL Slater, M Kalyan, D Ruano-Gall, O Marchès, LÁ Fernández, G Frankel, AR Shenoy
    Cell Rep, 2019;27(4):1008-1017.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation
    Authors: Q Lin, S Li, N Jiang, X Shao, M Zhang, H Jin, Z Zhang, J Shen, Y Zhou, W Zhou, L Gu, R Lu, Z Ni
    Redox Biol, 2019;26(0):101254.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes
    Authors: L Kasper, A König, PA Koenig, MS Gresnigt, J Westman, RA Drummond, MS Lionakis, O Gro beta, J Ruland, JR Naglik, B Hube
    Nat Commun, 2018;9(1):4260.
  19. ROR? controls inflammatory state of human macrophages
    Authors: N Nejati Moh, E Bjørkøy Ta, L Ryan, T Espevik, V Boyartchuk
    PLoS ONE, 2018;13(11):e0207374.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
    Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
    Autophagy, 2017;0(0):0.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii
    Authors: K Hendrawan, CM Whittingto, MC Brandley, K Belov, MB Thompson
    J. Exp. Zool. B Mol. Dev. Evol, 2017;0(0):.
    Species: Lizard
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition
    Authors: HL Young, EJ Rowling, M Bugatti, E Giurisato, N Luheshi, I Arozarena, JC Acosta, J Kamarashev, DT Frederick, ZA Cooper, A Reuben, J Gil, KT Flaherty, JA Wargo, W Vermi, MP Smith, C Wellbrock, A Hurlstone
    J. Exp. Med., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells
    Authors: JH Jeong, J Kim, J Kim, HR Heo, JS Jeong, YJ Ryu, Y Hong, SS Han, SH Hong, SJ Lee, WJ Kim
    Tuberc Respir Dis (Seoul), 2017;80(3):247-254.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
    Authors: Yong Hwan Park
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  25. 17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
    Sci Rep, 2016;6(0):37625.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  27. Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
    Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
    Cytokine, 2015;71(2):223-31.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  28. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
    Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
    Eur J Immunol, 2015;45(4):1092-102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1beta for Caspase-1 Cleavage.
    Authors: Edye M, Brough D, Allan S
    J Biol Chem, 2015;290(42):25374-81.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  30. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
    Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
    Nat Med, 2015;21(3):248-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.
    Authors: Kortmann J, Brubaker S, Monack D
    J Immunol, 2015;195(3):815-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  32. Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
    Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J
    J Immunol, 2015;194(11):5472-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014;2014(0):593562.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  34. Complement C5a potentiates uric acid crystal-induced IL-1beta production.
    Authors: An L, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M
    Eur J Immunol, 2014;44(12):3669-79.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
    Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
    J Lipid Res, 2013;54(11):2998-3008.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  36. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
    Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J
    Nature, 2012;492(7427):123-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  37. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
    Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
    J. Immunol., 2011;186(7):4175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  38. ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo.
    Authors: Rocks N, Paulissen G, Quesada-Calvo F, Munaut C, Gonzalez ML, Gueders M, Hacha J, Gilles C, Foidart JM, Noel A, Cataldo DD
    Cancer Res., 2008;68(22):9541-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
    Authors: Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A
    Cytokine, 2008;44(3):352-60.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-P
  40. Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
    Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
    Biol. Reprod., 2007;76(6):1009-15.
    Species: Amphibian
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  41. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004;80(2):231-5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  42. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human IL-1 beta /IL-1F2 Antibody
By Karina Krotova on 04/13/2017
Application: Western Blot Sample Tested: monocyte derived macrophages,THP-1 human acute monocytic leukemia cell line Species: Human

These Abs recognize both pro-IL1b (p31) and active, cleaved form (p17) in cell lysates and conditioned media of activated human monocyte derived macrophages and THP-1 derived macrophages. To see p17 IL-1b I used 10% tricine gel due to small size of detected protein.
I use antibody at dilution 1:500


Human IL‑1 beta /IL‑1F2 Antibody
By Anonymous on 11/24/2015
Application: Acoustic Assay on the BioScale ViBE Platform Sample Tested: Human Plasma,Plasma Species: Human