Human IL-1 beta /IL-1F2 Antibody

Catalog # Availability Size / Price Qty
AF-201-NA
AF-201-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Best Seller
IL-1 beta /IL-1F2 in Human Tonsil Using Dual RNAscope®ISH and IHC.
12 Images
Product Details
Citations (48)
FAQs
Supplemental Products
Reviews (2)

Human IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 beta /IL-1F2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
Ala117-Ser269
Accession # NP_000567
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
TF‑1 human erythroleukemic cell line
Immunohistochemistry
1-15 µg/mL
See below
Dual RNAscope ISH-IHC
1-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 6-42 ng/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Dual RNAscope ISH-IHC Compatible IL-1 beta /IL-1F2 antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

IL-1 beta /IL-1F2 in Human Tonsil Using Dual RNAscope®ISH and IHC. IL-1 beta /IL-1F2 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 310361) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Western Blot Detection of Human IL-1 beta /IL-1F2 antibody by Western Blot. View Larger

Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry IL-1 beta /IL-1F2 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IL‑1 beta /IL‑1F2 in Human Tonsil. IL-1 beta /IL-1F2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 39 kDa (as indicated) using 1 µg/mL of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). The ND50 is typically 6-42 ng/mL.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot BTK inhibitors and its dysfunctional mutation suppress NLRP3 inflammasome activation.(a) Enzyme-linked immunosorbent assay (ELISA) of human IL-1 beta in supernatants and immunoblot analysis of human IL-1 beta p17, caspase-1 p20/p22 in supernatants and pro-IL-1 beta in cell lysates of THP-1-Mφs that were pretreated with the indicated inhibitors for 30 min and then stimulated with alum for 6 h. ELISA of murine IL-1 beta (b,c) and TNF-alpha (b) in supernatants of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum for 3 h. (d,e) Immunoblot analysis of the indicated proteins (d) and ELISA of murine IL-1 beta and IL-6 in supernatants of LPS-primed peritoneal macrophages from Xid and WT mice stimulated with alum for 6 h. (f) ELISA of murine IL-1 beta in supernatants of LPS-primed murine peritoneal macrophages pretreated with LFM-A13 and/or Syk inhibitor (R406), then stimulated with alum for 3 h. (g) ELISA of murine IL-1 beta and immunoblot analysis of murine caspase-1 p20 in supernatant of LPS-primed murine peritoneal macrophages stimulated with the indicated NLRP3 inflammasome activators for 3 h. Immunoblot analysis of murine IL-1 beta p17, caspase-1 p20 in supernatants, pro-IL-1 beta, pro-caspase-1 and ASC in cell lysates (h), and ELISA of murine IL-1 beta in supernatants (i) of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum or poly(dA:dT) for 3 h. Data are representative of three independent experiments. Data are presented as mean±s.d. (triplicate). **P<0.01; ***P<0.003. Two-sided Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26059659), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot BTK inhibitors and its dysfunctional mutation suppress NLRP3 inflammasome activation.(a) Enzyme-linked immunosorbent assay (ELISA) of human IL-1 beta in supernatants and immunoblot analysis of human IL-1 beta p17, caspase-1 p20/p22 in supernatants and pro-IL-1 beta in cell lysates of THP-1-Mφs that were pretreated with the indicated inhibitors for 30 min and then stimulated with alum for 6 h. ELISA of murine IL-1 beta (b,c) and TNF-alpha (b) in supernatants of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum for 3 h. (d,e) Immunoblot analysis of the indicated proteins (d) and ELISA of murine IL-1 beta and IL-6 in supernatants of LPS-primed peritoneal macrophages from Xid and WT mice stimulated with alum for 6 h. (f) ELISA of murine IL-1 beta in supernatants of LPS-primed murine peritoneal macrophages pretreated with LFM-A13 and/or Syk inhibitor (R406), then stimulated with alum for 3 h. (g) ELISA of murine IL-1 beta and immunoblot analysis of murine caspase-1 p20 in supernatant of LPS-primed murine peritoneal macrophages stimulated with the indicated NLRP3 inflammasome activators for 3 h. Immunoblot analysis of murine IL-1 beta p17, caspase-1 p20 in supernatants, pro-IL-1 beta, pro-caspase-1 and ASC in cell lysates (h), and ELISA of murine IL-1 beta in supernatants (i) of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum or poly(dA:dT) for 3 h. Data are representative of three independent experiments. Data are presented as mean±s.d. (triplicate). **P<0.01; ***P<0.003. Two-sided Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26059659), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry Pyroptosis/IL-1 beta pathway is enhanced in vivo and can be modulated by anakinra. a In the PI model, phosphorylated-p65 NF-kappa B, NLRP3, 8-OHdG, IL-1 beta, and TUNEL were detected by IHC (Bar, 100 μm). b IL-1 beta was immuno-localized in gum tissues of mice with PI alone and with orthotopic mammary tumor introduction (Bar, 100 μm). c The mRNA expression of IL-1 beta was measured in the gingiva of the indicated groups. Serum IL-1 beta was detected by ELISA (n ≥ 5; Student’s t test). d The size of primary tumors was measured (n ≥ 7). e Early metastasis in lymph nodes were measured by luciferase assay and normalized by protein concentration, where anakinra reduced the metastasis to the axillary and cervical lymph nodes. MDSC content was also decreased by anakinra in cervical lymph node. (n ≥ 7; Ank anakinra; ANOVA, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31685946), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human IL-1 beta/IL-1F2 by Immunohistochemistry View Larger

Detection of Human IL-1 beta/IL-1F2 by Immunohistochemistry TMZ inhibits tumor growth and enhances the expression of NLRP1, IL-1 beta, and Notch1 in 1205Lu tumors in vivo. (A) Tumor growth curve of 1205Lu parental cells injected subcutaneously in mice, intraperitoneally treated with a TMZ cycle or 10% dimethyl sulfoxide (DMSO) (Control) (indicated by arrows). Tumor growth was monitored for 26 days. Data are expressed as the mean ± SEM. n = 10 tumors (control) or 12 tumors (TMZ). (B) qRT-PCR analysis of NLRP1 expression in single tumor cells isolated from the tumor tissues studied in (A). Data are expressed as the mean ± SEM. n = 3 samples. * p < 0.05 and *** p < 0.001 vs the corresponding tumors. (C–E) Immunohistochemical staining of tumor tissues with NLRP1 (C), IL-1 beta (D), and Notch1 (E). Samples MB656, MB657, and MB658 were from the control group, whereas MB659, MB660, and MB661 were from the TMZ group. Bar = 50 μm (left panels, 20×) or 20 μm (right panels, 63×). Representative images are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32899791), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot BTK inhibitors and its dysfunctional mutation suppress NLRP3 inflammasome activation.(a) Enzyme-linked immunosorbent assay (ELISA) of human IL-1 beta in supernatants and immunoblot analysis of human IL-1 beta p17, caspase-1 p20/p22 in supernatants and pro-IL-1 beta in cell lysates of THP-1-Mφs that were pretreated with the indicated inhibitors for 30 min and then stimulated with alum for 6 h. ELISA of murine IL-1 beta (b,c) and TNF-alpha (b) in supernatants of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum for 3 h. (d,e) Immunoblot analysis of the indicated proteins (d) and ELISA of murine IL-1 beta and IL-6 in supernatants of LPS-primed peritoneal macrophages from Xid and WT mice stimulated with alum for 6 h. (f) ELISA of murine IL-1 beta in supernatants of LPS-primed murine peritoneal macrophages pretreated with LFM-A13 and/or Syk inhibitor (R406), then stimulated with alum for 3 h. (g) ELISA of murine IL-1 beta and immunoblot analysis of murine caspase-1 p20 in supernatant of LPS-primed murine peritoneal macrophages stimulated with the indicated NLRP3 inflammasome activators for 3 h. Immunoblot analysis of murine IL-1 beta p17, caspase-1 p20 in supernatants, pro-IL-1 beta, pro-caspase-1 and ASC in cell lysates (h), and ELISA of murine IL-1 beta in supernatants (i) of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum or poly(dA:dT) for 3 h. Data are representative of three independent experiments. Data are presented as mean±s.d. (triplicate). **P<0.01; ***P<0.003. Two-sided Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26059659), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human IL-1 beta/IL-1F2 by Immunohistochemistry View Larger

Detection of Human IL-1 beta/IL-1F2 by Immunohistochemistry IL-1 and IL-1R1 expression is enriched in the melanoma stroma. (A) Real-time qPCR analysis of IL1A and IL1B expression in stage-III and stage-IV melanoma tumor samples (n = 39) relative to expression in human skin samples (n = 8). ***, P < 0.001; Mann-Whitney test. (B) Analysis of IL1B expression in normal skin and benign nevi samples (nonmalignant; n = 25) and cutaneous melanoma samples (malignant; n = 45) from an available gene expression dataset (Talantov et al., 2005) accessed through the Oncomine platform. (C) Sections from a case of primary cutaneous melanoma stained for IL-1 beta, CD163, and CD68 expression as indicated by labels. Bars: (i) 200 µm; (ii) 50 µm; (iii) 33 µm. (D) Serial sections from two skin metastases (i–iii and iv–vi, respectively), stained for IL-1R1 and SMA expression as indicated by the labels. Bars: (i and iv) 200 µm; (ii, iii, v, and vi) 33 µm. (C and D) Arrowheads indicate cells that are clearly double stained. (E) Western blot analysis of IL-1R1 and IL-1 beta precursor protein expression in a panel of cell lines. Data are representative of three independent experiments. (F) Secreted IL-1 beta in conditioned media from a panel of melanoma cell lines detected by ELISA. Data are represented as mean ± SEM for three independent samples in each group. **, P < 0.01; Dunn’s multiple comparisons test. (E and F) Macrophages (Mφ) were stimulated with 100 ng/ml IFN-gamma and 20 ng/ml LPS. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28450382), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

You must select a language.

x

Citations for Human IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

48 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. HIF1alpha-BNIP3-mediated mitophagy protects against renal fibrosis by decreasing ROS and inhibiting activation of the NLRP3 inflammasome
    Authors: J Li, Q Lin, X Shao, S Li, X Zhu, J Wu, S Mou, L Gu, Q Wang, M Zhang, K Zhang, J Lu, Z Ni
    Cell Death & Disease, 2023-03-17;14(3):200.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
    Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
    Nature Immunology, 2022-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. NLRP3 inflammasome triggers interleukin-37 release from human monocytes
    Authors: A Gritsenko, R Díaz-Pino, G Lopez-Cast
    European Journal of Immunology, 2022-04-28;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. IBD-associated G protein-coupled receptor 65 variant compromises signalling and impairs key functions involved in inflammation
    Authors: V Mercier, G Boucher, D Devost, K Bourque, A Alikashani, C Beauchamp, A Bitton, S Foisy, P Goyette, G Charron, TE Hébert, JD Rioux
    Cellular Signalling, 2022-02-24;93(0):110294.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome
    Authors: SD Rao, Q Chen, Q Wang, EL Orth-He, M Saoi, AR Griswold, A Bhattachar, DP Ball, HC Huang, AJ Chui, DJ Covelli, S You, JR Cross, DA Bachovchin
    Nature Chemical Biology, 2022-02-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Single cell transcriptomic landscape of diabetic foot ulcers
    Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
    Nature Communications, 2022-01-10;13(1):181.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
    Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
    Redox Biology, 2021-12-05;48(0):102205.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Dot Blot
  8. Endolysosomal ion channel MCOLN2 (Mucolipin-2) promotes prostate cancer progression via IL-1beta/NF-kappaB pathway
    Authors: H Yu, M Xie, Z Meng, CY Lo, FL Chan, L Jiang, X Meng, X Yao
    British Journal of Cancer, 2021-09-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. The Blockade of Tumoral IL1&beta-Mediated Signaling in Normal Colonic Fibroblasts Sensitizes Tumor Cells to Chemotherapy and Prevents Inflammatory CAF Activation
    Authors: NG Díaz-Marot, G Garcia-Vic, G Polcaro, M Bañuls, N Albert, A Villanueva, DG Molleví
    International Journal of Molecular Sciences, 2021-05-07;22(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Gasdermin E permits interleukin-1 beta release in distinct sublytic and pyroptotic phases
    Authors: B Zhou, DW Abbott
    Cell Reports, 2021-04-13;35(2):108998.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Glycyrrhizin prevents SARS-CoV-2 S1 and Orf3a induced high mobility group box 1 (HMGB1) release and inhibits viral replication
    Authors: P Gowda, S Patrick, SD Joshi, RK Kumawat, E Sen
    Cytokine, 2021-03-12;142(0):155496.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8
    Authors: Q Gong, K Robinson, C Xu, PT Huynh, KHC Chong, EYJ Tan, J Zhang, ZZ Boo, DET Teo, K Lay, Y Zhang, JSY Lim, WI Goh, G Wright, FL Zhong, B Reversade, B Wu
    Nature Communications, 2021-01-08;12(1):188.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Inflammasome Sensor NLRP1 Confers Acquired Drug Resistance to Temozolomide in Human Melanoma
    Authors: Z Zhai, JM Samson, T Yamauchi, PK Vaddi, Y Matsumoto, CA Dinarello, D Ravindran, M Fujita
    Cancers (Basel), 2020-09-04;12(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Platelets Fuel the Inflammasome Activation of Innate Immune Cells
    Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
    Cell Rep, 2020-05-12;31(6):107615.
    Species: Human
    Sample Types:
    Applications: Blocking/Neutralizing
  15. Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin in?ammation
    Authors: Z Wang, H Zhou, H Zheng, X Zhou, G Shen, X Teng, X Liu, J Zhang, X Wei, Z Hu, F Zeng, Y Hu, J Hu, X Wang, S Chen, J Cheng, C Zhang, Y Gui, S Zou, Y Hao, Q Zhao, W Wu, Y Zhou, K Cui, N Huang, Y Wei, W Li, J Li
    Autophagy, 2020-02-16;0(0):1-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Interleukin 1&beta/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy
    Authors: Y Chen, Z Yang, B Deng, D Wu, Y Quan, Z Min
    Oncol. Rep., 2020-01-22;43(3):908-918.
    Species: Human
    Sample Types: Cell Culture Lysates, Whole Tissue
    Applications: IHC, Western Blot
  17. Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells
    Authors: R Cheng, S Billet, C Liu, S Haldar, D Choudhury, M Tripathi, M Hav, A Merchant, T Hu, H Huang, H Zhou, NA Bhowmick
    Oncogene, 2019-11-04;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysate, Whole Tissue
    Applications: IHC, Western Blot
  18. PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation
    Authors: Q Lin, S Li, N Jiang, X Shao, M Zhang, H Jin, Z Zhang, J Shen, Y Zhou, W Zhou, L Gu, R Lu, Z Ni
    Redox Biol, 2019-06-11;26(0):101254.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration
    Authors: C De Witte, K Demeyere, S De Bruycke, B Taminiau, G Daube, R Ducatelle, E Meyer, F Haesebrouc
    Vet. Res., 2019-05-24;50(1):39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages
    Authors: PJ Goddard, J Sanchez-Ga, SL Slater, M Kalyan, D Ruano-Gall, O Marchès, LÁ Fernández, G Frankel, AR Shenoy
    Cell Rep, 2019-04-23;27(4):1008-1017.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1? Protein during Virion-Mediated Activation of the AIM2 Inflammasome
    Authors: S Botto, J Abraham, N Mizuno, K Pryke, B Gall, I Landais, DN Streblow, KJ Fruh, VR DeFilippis
    MBio, 2019-02-12;10(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  22. ROR? controls inflammatory state of human macrophages
    Authors: N Nejati Moh, E Bjørkøy Ta, L Ryan, T Espevik, V Boyartchuk
    PLoS ONE, 2018-11-28;13(11):e0207374.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes
    Authors: L Kasper, A König, PA Koenig, MS Gresnigt, J Westman, RA Drummond, MS Lionakis, O Gro beta, J Ruland, JR Naglik, B Hube
    Nat Commun, 2018-10-15;9(1):4260.
  24. ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells
    Authors: JH Jeong, J Kim, J Kim, HR Heo, JS Jeong, YJ Ryu, Y Hong, SS Han, SH Hong, SJ Lee, WJ Kim
    Tuberc Respir Dis (Seoul), 2017-07-03;80(3):247-254.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition
    Authors: HL Young, EJ Rowling, M Bugatti, E Giurisato, N Luheshi, I Arozarena, JC Acosta, J Kamarashev, DT Frederick, ZA Cooper, A Reuben, J Gil, KT Flaherty, JA Wargo, W Vermi, MP Smith, C Wellbrock, A Hurlstone
    J. Exp. Med., 2017-04-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii
    Authors: K Hendrawan, CM Whittingto, MC Brandley, K Belov, MB Thompson
    J. Exp. Zool. B Mol. Dev. Evol, 2017-03-10;0(0):.
    Species: Lizard
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
    Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
    Autophagy, 2017-01-01;0(0):0.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. 17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
    Sci Rep, 2016-11-24;6(0):37625.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation
    J Immunol, 2016-08-12;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  30. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
    Authors: Yong Hwan Park
    Nat Immunol, 2016-06-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  31. Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1beta for Caspase-1 Cleavage.
    Authors: Edye M, Brough D, Allan S
    J Biol Chem, 2015-08-31;290(42):25374-81.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  32. Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.
    Authors: Kortmann J, Brubaker S, Monack D
    J Immunol, 2015-06-24;195(3):815-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  33. Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
    Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J
    J Immunol, 2015-04-27;194(11):5472-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
    Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
    Nat Med, 2015-02-16;21(3):248-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
    Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
    Eur J Immunol, 2015-01-19;45(4):1092-102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
    Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
    Cytokine, 2014-11-21;71(2):223-31.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  37. Complement C5a potentiates uric acid crystal-induced IL-1beta production.
    Authors: An L, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M
    Eur J Immunol, 2014-10-28;44(12):3669-79.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Early mucosal sensing of SIV infection by paneth cells induces IL-1beta production and initiates gut epithelial disruption.
    Authors: Hirao L, Grishina I, Bourry O, Hu W, Somrit M, Sankaran-Walters S, Gaulke C, Fenton A, Li J, Crawford R, Chuang F, Tarara R, Marco M, Baumler A, Cheng H, Dandekar S
    PLoS Pathog, 2014-08-28;10(8):e1004311.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  39. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014-03-31;2014(0):593562.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  40. Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
    Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
    J Lipid Res, 2013-09-03;54(11):2998-3008.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  41. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
    Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J
    Nature, 2012-11-11;492(7427):123-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  42. Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
    Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
    Haematologica, 2011-05-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  43. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
    Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
    J. Immunol., 2011-03-02;186(7):4175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  44. ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo.
    Authors: Rocks N, Paulissen G, Quesada-Calvo F, Munaut C, Gonzalez ML, Gueders M, Hacha J, Gilles C, Foidart JM, Noel A, Cataldo DD
    Cancer Res., 2008-11-15;68(22):9541-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  45. Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
    Authors: Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A
    Cytokine, 2008-11-12;44(3):352-60.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-P
  46. Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
    Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
    Biol. Reprod., 2007-02-21;76(6):1009-15.
    Species: Amphibian
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  47. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004-09-01;80(2):231-5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  48. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003-12-21;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IL-1 beta /IL-1F2 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

5 Star
50%
4 Star
50%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human IL-1 beta /IL-1F2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human IL-1 beta /IL-1F2 Antibody
By Karina Krotova on 04/13/2017
Application: WB Sample Tested: monocyte derived macrophages,THP-1 human acute monocytic leukemia cell line Species: Human

These Abs recognize both pro-IL1b (p31) and active, cleaved form (p17) in cell lysates and conditioned media of activated human monocyte derived macrophages and THP-1 derived macrophages. To see p17 IL-1b I used 10% tricine gel due to small size of detected protein.
I use antibody at dilution 1:500


Human IL‑1 beta /IL‑1F2 Antibody
By Anonymous on 11/24/2015
Application: Acoustic Assay Sample Tested: Human Plasma,Plasma Species: Human