Dopamine Receptors

Dopamine is the major catecholamine neurotransmitter present in the mammalian brain where it is responsible for a variety of functions, including locomotion, neuroendocrine secretion, cognition and emotion. Dopamine also plays a role in the periphery where it regulates a range of other processes, including catecholamine release, hormone secretion, and gastrointestinal motility.

The application of molecular cloning techniques to the study of dopamine receptors has expanded the classical dopamine D1/D2 classification scheme to include five dopamine receptor subtypes. These may be divided into D1-like (D1 and D5) and D2-like (D2, D3 and D4 receptors) based on their predicted transmembrane topologies, and functional and pharmacological properties. Dysregulation of dopamine transmission causes a variety of conditions such as Parkinson's disease, Tourette's syndrome, schizophrenia and hyperprolactemia.

Literature

Parkinson's Disease Life Science Poster

Parkinson's Disease Poster 

Parkinson's disease (PD) causes chronic disability and is the second most common neurodegenerative condition. This poster outlines the neurobiology of the disease, as well as highlighting current therapeutic treatments for symptomatic PD, and emerging therapeutic strategies to delay PD onset and progression.

Receptor Data

Dopamine Receptor Data

Receptor SubtypeD1-like ReceptorsD2-like Receptors
 D1D5D2D3D4
G proteinGsGsGi/oGi/oGi/o
Transduction Mechanism↑ AC, ↑ PLC, ↑ L-type Ca2+ channels↑ AC↓ AC, ↑ [Ca2+]i, K+ conductance, ↓ Ca2+ conductance↓ AC, activation of MAP kinase↓ AC, ↓ Ca2+ conductance
LocalizationCaudate, putamen, nucleus accumbens, olfactory tubercleHippocampus, hypothalamusCaudate, putamen, nucleus accumbens, olfactory tubercleNucleus accumbens, olfactory tubercle, islands of CallejaFrontal cortex, midbrain, amygdala, cardiovascular system
Likely Physiological RolesLocomotion, reward, reinforcement, learning and memory, renin secretionLearning and memoryLocomotion, reward, reinforcement, learning and memoryLocomotion, possible role in cognition and emotionMostly unknown - possible role in cognition and emotion, hypertension
Key CompoundsKi Values (nM)     
D1-like AgonistsSKF 38393 (0922)
SKF 83822 (2075)
1
3.2
~ 0.5
3.1
~ 150
186
~ 5000
66
~ 1000
335
D1-like AntagonistsSCH 23390 (0925)~ 0.20.3~ 1100~ 800~ 3000
D2-like AgonistsBromocriptine (0427)
7-HydroxyDPAT (0706)
Cabergoline (2664)
~ 440
~ 5000
-
~ 450
-
165
~ 8
10
0.7
~ 5
~ 1
1.5
~ 290
650
9.0
D2-like AntagonistsClozapine (0444)
Haloperidol (0931)
Nemonapride (1746)
NGB 2904 (2635)
Raclopride (1810)
Remoxipride (0916)
Spiperone (0995)
(S)-(-)-Sulpride (0895)
 
~ 170
~ 80
-
> 10000
18000
-
~ 350
~ 45000
~ 330
~ 100
-
217
-
-
~ 3500
77000
~ 230
1.2
0.06
1.4
1.8
~ 300
0.06
~ 15
~ 170
~ 7
0.3
> 5000
3.5
~ 1600
0.6
~ 13
21
2.3
0.15
> 10000
2400
~ 2800
0.08
1000

All Ki values for agonists were determined at the high-affinity state for all receptors, except for D3 receptors.

References

Seeman and Van Tol (1994) Dopamine receptor pharmacology. TiPS 15 264. Missale et al (1998) Dopamine receptors: from structure to function. Physiol.Rev. 78 189. O'Sullivan et al (2004) SK&F 83822 distinguishes adenylyl cyclase from phospholipase C-coupled D1-like receptors: behavioural topography. Eur.J.Pharmacol. 486 273. Kvernmo et al (2006) A review of the receptor-binding and pharmacokinetic properties of dopamine agonists. Clin.Ther. 28 1065. Yuan et al (1998) NGB 2904 and NGB 2849: two highly selective dopamine D3 receptor antagonists. Bioorg.Med.Chem.Lett. 8 2715.