Human IL-6 Antibody

Catalog # Availability Size / Price Qty
MAB2061-SP
MAB2061-100
MAB2061-500
IL‑6 in Human PBMCs.
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Product Details
Citations (14)
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Reviews (1)

Human IL-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.
Source
Monoclonal Mouse IgG2B Clone # 1936
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑6
Val30-Met212
Accession # P05231
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry IL‑6 in Human PBMCs. View Larger

IL‑6 in Human PBMCs. IL‑6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IL‑6  in Human Appendix. View Larger

IL‑6 in Human Appendix. IL‑6 was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection  of IL‑6 in Human PBMCs by Flow Cytometry. View Larger

Detection of IL‑6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with
100 μg/mL LPS for 24 hours were stained with Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061). The ND50 is typically 0.05-0.15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22‑28 kDa phosphorylated and variably glycosylated molecule (1‑4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6‑9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans‑signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3).
Trans‑signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans‑signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).

References
  1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
  2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
  3. Jones, S.A. (2005) J. Immunol. 175:3468.
  4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
  5. Hirano, T. et al. (1986) Nature 324:73.
  6. Alberti, L. et al. (2005) Cancer Res. 65:2.
  7. Kestler, D.P. et al. (1995) Blood 86:4559.
  8. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
  9. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
  10. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.
  11. Murakami, M. et al. (1993) Science 260:1808.
  12. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  13. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125. 
Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Product Datasheets

Citations for Human IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer
    Authors: N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti
    Oncogene, 2018;37(29):4013-4032.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  2. Oral fibroblasts modulate the macrophage response to bacterial challenge
    Authors: R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum
    Sci Rep, 2017;7(1):11516.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017;12(4):e0175712.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
    Authors: Wan-Chien Cheng
    Eur J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. NOTCH1 mediates a switch between two distinct secretomes during senescence
    Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
    Nat. Cell Biol, 2016;18(9):979-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor.
    Authors: Becker, T M, Boyd, S C, Mijatov, B, Gowrishankar, K, Snoyman, S, Pupo, G M, Scolyer, R A, Mann, G J, Kefford, R F, Zhang, X D, Rizos, H
    Oncogene, 2014;33(9):1158-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. IL-1alpha released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts.
    Authors: Suwara M, Green N, Borthwick L, Mann J, Mayer-Barber K, Barron L, Corris P, Farrow S, Wynn T, Fisher A, Mann D
    Mucosal Immunol, 2014;7(3):684-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
    Authors: Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T
    Mod Pathol, 2012;26(3):357-69.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  9. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation.
    Authors: Steinbicker AU, Sachidanandan C, Vonner AJ
    Blood, 2011;117(18):4915-23.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
    Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
    J. Allergy Clin. Immunol., 2009;123(1):239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion.
    Authors: Rodier F, Coppe JP, Patil CK, Hoeijmakers WA, Munoz DP, Raza SR, Freund A, Campeau E, Davalos AR, Campisi J
    Nat. Cell Biol., 2009;11(8):973-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Interleukin-6 as a photoreceptor neuroprotectant in an experimental model of retinal detachment.
    Authors: Chong DY, Boehlke CS, Zheng QD, Zhang L, Han Y, Zacks DN
    Invest. Ophthalmol. Vis. Sci., 2008;49(7):3193-200.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  14. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005;46(6):659-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human IL-6 Antibody

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Human IL‑6 Antibody
By Anonymous on 04/29/2016
Application: ELISA Sample Tested: Mouse ear fibroblast Species: Human