Human IL-6 Antibody

Catalog # Availability Size / Price Qty
MAB2061-SP
MAB2061-100
MAB2061-500
IL‑6 in Human Appendix.
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Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (2)

Human IL-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.
Source
Monoclonal Mouse IgG2B Clone # 1936
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑6
Val30-Met212
Accession # P05231
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-15 µg/mL
Immersion fixed Human PBMCs stimulated with LPS (positive control) and untreated human PBMCs (negative control)
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
PBMCs treated LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005), and PBMC stimulated with 100 ng/ml of LPS and Brefeldin A for 24 hrs or unstimulated
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. The Neutralization Dose (ND50) is typically 8.00-80.0 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL-6 antibody in Human Appendix by Immunohistochemistry (IHC-P). View Larger

IL‑6 in Human Appendix. IL-6 was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of IL-6 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 100 ng/mL LPS for 24 hours were stained with Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061, filled histogram) or isotype control antibody (MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL-6 (206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061). The ND50 is typically 8.00-80.0 ng/mL.

Immunocytochemistry View Larger

Detection of IL‑6 in Human PBMCs. IL‑6 was detected in immersion fixed Human PBMCs stimulated with LPS (positive control) and absent in untreated human PBMCs (negative control) using Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry View Larger

Detection of IL‑6 PBMC Cells by Flow Cytometry. PBMC cells stimulated with 100 ng/ml LPS and Brefeldin A for 24 hrs (A) or unstimulated (B) were stained with Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Citations for Human IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Head and neck squamous cancer cells enhance the differentiation of human mesenchymal stem cells to adipogenic and osteogenic linages in vitro
    Authors: TJ Meyer, S Hackenberg, M Herrmann, T Gehrke, M Steber, R Hagen, N Kleinsasse, A Scherzad
    Oncology Letters, 2022-10-31;24(6):450.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
    Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
    Nature Communications, 2022-10-14;13(1):6078.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Systemic Immune Dysfunction in Cancer Patients Driven by IL6 Induction of LAG3 in Peripheral CD8+ T Cells.
    Authors: Somasundaram A, Cillo A, Lampenfeld C, Workman C, Kunning S, Oliveri L, Velez M, Joyce S, Calderon M, Dadey R, Rajasundaram D, Normolle D, Watkins S, Herman J, Kirkwood J, Lipson E, Ferris R, Bruno T, Vignali D
    Cancer Immunol Res, 2022-07-01;10(7):885-899.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
    Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
    Nature Communications, 2022-06-03;13(1):3121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Orally-active, clinically-translatable senolytics restore alpha-Klotho in mice and humans
    Authors: Y Zhu, LGPL Prata, EOW Gerdes, JME Netto, T Pirtskhala, N Giorgadze, U Tripathi, CL Inman, KO Johnson, A Xue, AK Palmer, T Chen, K Schaefer, JN Justice, AM Nambiar, N Musi, SB Kritchevsk, J Chen, S Khosla, D Jurk, MJ Schafer, T Tchkonia, JL Kirkland
    EBioMedicine, 2022-03-13;0(0):103912.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Metabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survival
    Authors: J Whitburn, SR Rao, EV Morris, S Tabata, A Hirayama, T Soga, JR Edwards, Z Kaya, C Palmer, FC Hamdy, CM Edwards
    Science Advances, 2022-02-25;8(8):eabf9096.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
    Authors: T Fukasawa, A Yoshizaki, S Ebata, A Yoshizaki-, Y Asano, A Enomoto, K Miyagawa, Y Kazoe, K Mawatari, T Kitamori, S Sato
    Elife, 2021-12-02;10(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. COX2 regulates senescence secretome composition and senescence surveillance through PGE2
    Authors: S Gonçalves, K Yin, Y Ito, A Chan, I Olan, S Gough, L Cassidy, E Serrao, S Smith, A Young, M Narita, M Hoare
    Cell Reports, 2021-03-16;34(11):108860.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. Piezo1 Channels Contribute to the Regulation of Human Atrial Fibroblast Mechanical Properties and Matrix Stiffness Sensing
    Authors: R Emig, W Knodt, MJ Krussig, CM Zgierski-J, O Gorka, O Gro beta, P Kohl, U Ravens, R Peyronnet
    Cells, 2021-03-16;10(3):.
    Species: Human
    Sample Types: Protein
    Applications: Neutralization
  10. Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling
    Authors: R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke
    Nat Commun, 2019-11-01;10(1):5016.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells
    Authors: LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith
    Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4652-4660.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Targeting TGFbetaR2-mutant tumors exposes vulnerabilities to stromal TGFbeta blockade in pancreatic cancer
    Authors: H Huang, Y Zhang, V Gallegos, N Sorrelle, MM Zaid, J Toombs, W Du, S Wright, M Hagopian, Z Wang, AN Hosein, AA Sathe, C Xing, EJ Koay, KE Driscoll, RA Brekken
    EMBO Mol Med, 2019-10-14;0(0):e10515.
    Species: Human
    Sample Types: Protein Lysates
    Applications: Western Blot
  13. Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells
    Authors: MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani
    Sci Rep, 2019-07-10;9(1):10007.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  15. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer
    Authors: N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti
    Oncogene, 2018-04-26;37(29):4013-4032.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  16. Phosphatase of regenerating liver-3 (PRL-3) is overexpressed in classical Hodgkin lymphoma and promotes survival and migration
    Authors: MA Hjort, H Hov, P Abdollahi, EN Vandsemb, UM Fagerli, B Lund, TS Slørdahl, M Børset, TB Rø
    Exp Hematol Oncol, 2018-04-10;7(0):8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Oral fibroblasts modulate the macrophage response to bacterial challenge
    Authors: R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum
    Sci Rep, 2017-09-14;7(1):11516.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017-04-14;12(4):e0175712.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. NOTCH1 mediates a switch between two distinct secretomes during senescence
    Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
    Nat. Cell Biol, 2016-08-15;18(9):979-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
    Authors: Wan-Chien Cheng
    Eur J Immunol, 2016-08-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. IL-1alpha released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts.
    Authors: Suwara M, Green N, Borthwick L, Mann J, Mayer-Barber K, Barron L, Corris P, Farrow S, Wynn T, Fisher A, Mann D
    Mucosal Immunol, 2013-10-30;7(3):684-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor.
    Authors: Becker, T M, Boyd, S C, Mijatov, B, Gowrishankar, K, Snoyman, S, Pupo, G M, Scolyer, R A, Mann, G J, Kefford, R F, Zhang, X D, Rizos, H
    Oncogene, 2013-03-04;33(9):1158-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
    Authors: Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T
    Mod Pathol, 2012-11-23;26(3):357-69.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012-09-25;10(9):e1001395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation.
    Authors: Steinbicker AU, Sachidanandan C, Vonner AJ
    Blood, 2011-03-10;117(18):4915-23.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  26. Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion.
    Authors: Rodier F, Coppe JP, Patil CK, Hoeijmakers WA, Munoz DP, Raza SR, Freund A, Campeau E, Davalos AR, Campisi J
    Nat. Cell Biol., 2009-07-13;11(8):973-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
    Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
    J. Allergy Clin. Immunol., 2008-12-05;123(1):239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Interleukin-6 as a photoreceptor neuroprotectant in an experimental model of retinal detachment.
    Authors: Chong DY, Boehlke CS, Zheng QD, Zhang L, Han Y, Zacks DN
    Invest. Ophthalmol. Vis. Sci., 2008-03-31;49(7):3193-200.
    Species: Mouse, Rat
    Sample Types: In Vivo
    Applications: Neutralization
  29. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005-06-01;46(6):659-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human IL-6 Antibody

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Human IL-6 Antibody
By Anonymous on 06/18/2022
Application: IHC Sample Tested: Colon tissue Species: Human

Human IL‑6 Antibody
By Jason Thall on 04/29/2016
Application: ELISA Sample Tested: Mouse ear fibroblast Species: Human