R&D Systems Proteome Profiler™ Human Chemokine Array Kit (Catalog # ARY017) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 31 chemokines in a single sample. No specialized equipment is necessary.
|Simultaneously detect the levels of these chemokines in a single sample.|
|CCL3/CCL4 (MIP-1 alpha/MIP-1 beta)||CCL26/Eotaxin-3||CXCL10/IP-10|
|CCL15/MIP-1 delta/LKN-1||CXCL1/GRO alpha||CXCL17/VCC-1|
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.