Antibodies for SARS-CoV-2 Detection

R&D Systems offers a range of antibodies that recognize and bind various SARS-CoV-2 antigens, such as the S1 and S2 subunits, as well as the receptor binding domain (RBD). These antibodies are highly validated and can be used to detect SARS-CoV-2 in multiple sample types by a variety of applications including ELISA, flow cytometry, and immunohistochemistry. Explore our range of SARS-CoV-2 antibodies and learn more about a specific antibody by clicking its catalog number.

Explore SARS-CoV-2 antibodies for:

• ELISA Development
• Flow Cytometry
• Immunohistochemistry (IHC)
• Western Blot

Additionally, visit our main Antibodies for Coronavirus Research page for more information on our antibodies and related resources for SARS-CoV-2 research.

SARS-CoV-2 Matched Antibody Pairs for ELISA Development

Using ELISAs to detect SARS-CoV-2 antigens allows for the detection and quantification of the viral proteins in samples. R&D Systems offers SARS-CoV-2 antibodies for researchers who want to develop their own ELISAs. Our ELISA-validated antibodies have been tested for SARS-CoV-2 antigen detection using a sandwich immunoassay, allowing us to recommend a matched antibody pair for assay development.

SARS-CoV-2 Antibodies for Flow Cytometry

Detection of SARS-CoV-2 antigens by flow cytometry offers a range of uses for SARS-CoV-2 research and COVID-19 therapy development. Flow detection of the viral antigens in concert with live/dead cell stains or other conjugated antibodies, such as those for cellular markers, allows for the characterization of infected cells as well as screening potential therapeutics for antibody binding to infected cells. R&D Systems offers SARS-CoV-2 antibodies validated for flow cytometry for use in SARS-CoV-2 research and therapy development.

Detection of SARS-CoV-2 Spike Protein by Flow Cytometry. HEK293 human embryonic kidney cell line, transfected with human ACE-2 and eGFP, was incubated with Recombinant SARS-CoV-2 Spike S1 Subunit His-Tag Protein (Catalog # 10522-CV), then stained with a Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (A; Catalog # MAB105403) or a Mouse IgG1 Isotype Control Antibody (B; Catalog # MAB002), followed by an APC-Conjugated Goat Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). SARS-CoV-2 Spike protein, bound to ACE-2 expressed on the surface of the transfected HEK293 cells, was detected.

SARS-CoV-2 Antibodies for Immunohistochemistry (IHC)

Detection of SARS-CoV-2 by IHC is a vital tool for researching SARS-CoV-2. Visualization of the SARS-CoV-2 proteins within cells and tissues by IHC allows for the semiquantitative identification of the virus while maintaining the spatial context of its expression, such as cell types targeted by SARS-CoV-2. R&D Systems offers SARS-CoV-2 antibodies validated for IHC for characterizing SARS-CoV-2 infection.

Spike RBD in SARS-CoV-2 Infected Human Lung. Spike RBD was detected in immersion-fixed, paraffin-embedded sections of SARS-CoV-2 infected human lung tissue using a Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802), followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Prior to incubation with the primary antibody, the tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). The tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.

SARS-CoV-2 Antibodies for Western Blot

Immunoblotting is an invaluable tool for COVID-19 research. Investigating mechanisms that allow SARS-CoV-2 to infect cells and escape immune surveillance while maintaining high infectivity often utilizes in vitro cell models with western blot analysis of SARS-CoV-2 proteins to confirm SARS-CoV-2 infection. Western blot is also used to determine which protein antigens are recognized by potential therapies such as antibody-based vaccines or sera from convalescent patients. R&D Systems offers SARS-CoV-2 antibodies validated for western blot for use in SARS-CoV-2 research and therapy development.

Detection of SARS-CoV-2 Spike S2 Subunit and SARS-CoV-2 Spike (Active Trimer) by Western Blot. Western blot shows Recombinant SARS-CoV-2 Spike S2 Subunit Protein (Catalog # 10584-CV; left blot) and Recombinant SARS-CoV-2 Spike (Active Trimer) Protein (Catalog # 10549-CV; right blot). The PVDF membrane was probed with a Mouse Anti-SARS-CoV-2 Spike S2 Subunit Monoclonal Antibody (Catalog # MAB10557) followed by an HRP-Conjugated Donkey Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SARS-CoV-2 Spike S2 Subunit at approximately 70 kDa (as indicated).

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