High-Quality Matched Antibody Pairs for ELISA & More
Develop more sensitive assays with high-affinity capture and detection antibody pairs from R&D Systems. Whether you're building a sandwich ELISA or developing a custom immunoassay, our matched pairs are optimized for accuracy, reproducibility, and flexibility.
What Are Matched Antibody Pairs?
Designed for ELISA and Sandwich Immunoassays
Matched antibody pairs are carefully selected capture and detection antibodies that work together to accurately detect specific analytes. They’re essential for reliable sandwich ELISA development and compatible with various immunoassay platforms.
Key Benefits:
- Expand beyond preconfigured kits with full control over your assay design
- Skip the screening step with antibodies optimized to work together
- Easy to integrate into custom workflows
- Scalable supply-- available to order in bulk
Featured Matched Pairs
| Target | Capture Antibody | Detection Antibody |
| Human IL-10 Antibody | MAB2172 | BAF217 |
| Human ErbB2/Her2 Antibody | MAB1129 | BAF1129 |
| Human BDNF Antibody | MAB848R | MAB648 |
Why Choose R&D Systems Antibody Pairs?
Our Antibody Pairs are Better from the Beginning
R&D Systems antibody experts know antibody quality starts at the beginning. Our industry-leading immunoassay antibodies are produced using full-length, bioactive recombinant proteins expressed in mammalian cells. This preserves native glycosylation and folding, resulting in more sensitive antibody pairs. Working with R&D Systems gives you access to expert technical support to guide antibody selection and assay development.
Our matched antibody pairs are compatible with:
- Plate-based sandwich ELISA development
- Luminex®, Simple Plex and other Multiplex Immunoassay platforms
- Custom biomarker validation studies
- Lateral flow assay development
FAQs:
What are matched antibody pairs?
Matched antibody pairs are carefully selected combinations of two antibodies that specifically bind to different epitopes on the same target protein—one for capture and one for detection. These pairs are optimized for use in sandwich ELISA assays, enabling highly specific and sensitive quantification of target proteins in complex biological samples such as serum, plasma, or cell culture supernatants. R&D Systems™ offers a wide range of high-affinity matched antibody pairs designed for reproducible immunoassay development and protein quantification workflows.
Can I customize an antibody pair?
Yes, R&D Systems™ offers custom antibody pairing services to support your specific assay development needs. If you're targeting a novel protein, working with a rare sample type, or need a specific detection format, our team can help identify and validate a custom capture and detection antibody combination tailored for your assay platform—whether you're building a sandwich ELISA, a multiplex assay, or another immunoassay format.
Are your antibody pairs validated for ELISA?
Yes, all R&D Systems™ matched antibody pairs are rigorously validated for use in ELISA applications. Validation includes testing for sensitivity, specificity, dynamic range, and inter-lot consistency to ensure reliable and reproducible results. Many of our antibody pairs are also backed by supporting data, including standard curves, recommended protocols, and validated sample types, making them ideal for both discovery research and assay development.