Proteome Profiler Mouse Cytokine Array Kit, Panel A

Contains 4 membranes - each spotted in duplicate with 40 different cytokine antibodies
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The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample.
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Citations (363)
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Proteome Profiler Mouse Cytokine Array Kit, Panel A Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

  • Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.

Principle of the Assay

The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.



Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.  

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
C5a IL-6 CCL2/JE/MCP-1
CCL1/I-309 IL-12 p70 CCL3/MIP-1 alpha
CCL11/Eotaxin IL-13 CCL4/MIP-1 beta
IL-1 alpha/IL-1F1 IL-23 CXCL12/SDF-1
IL-1 beta/IL-1F2 IL-27 CCL17/TARC
IL-1ra/IL-1F3 CXCL10/IP-10 TIMP-1
IL-2 CXCL11/I-TAC TNF-alpha


Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
C5a   DY2150
CCL1/I-309/TCA-3   DY845
CCL11/Eotaxin MME00 DY420
ICAM-1 MIC100 DY796
IFN-gamma MIF00 DY485
IL-1 alpha MLA00 DY400
IL-1 beta MLB00B DY401
IL-1ra MRA00 DY480
IL-2 M2000 DY402
IL-3 M3000 DY403
IL-4 M4000B DY404
IL-5 M5000 DY405
IL-6 M6000B DY406
IL-7 M7000 DY407
IL-10 M1000b DY417
IL-12 p70 M1270 DY419
IL-13 M1300CB DY413
IL-17 M1700 DY421
IL-23 M2300 DY1887
IL-27 M2728  
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CCL12/MCP-5 MCC120 DY428
CCL3/MIP-1 alpha MMA00 DY450
CCL4/MIP-1 beta MMB00 DY451
CXCL2/MIP-2 MM200 DY452
CXCL12/SDF-1 MCX120 DY460
CCL17/TARC   DY529
TIMP-1 MTM100 DY980
TNF-alpha MTA00 DY410
TREM-1 MTRM10 DY1187


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Scientific Data

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Citations for Proteome Profiler Mouse Cytokine Array Kit, Panel A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

363 Citations: Showing 1 - 10
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  1. Adenosine metabolized from extracellular ATP ameliorates organ injury by triggering A2BR signaling
    Authors: Kelestemur, T;Németh, ZH;Pacher, P;Beesley, J;Robson, SC;Eltzschig, HK;Haskó, G;
    Respiratory research  2023-07-13
  2. An LRP1-binding motif in cellular prion protein replicates cell-signaling activities of the full-length protein
    Authors: Mantuano, E;Zampieri, C;Azmoon, P;Gunner, CB;Heye, KR;Gonias, SL;
    JCI insight  2023-06-27
  3. Anti-inflammatory effects of Allium cepa L. peel extracts via inhibition of JAK-STAT pathway in LPS-stimulated RAW264.7 cells
    Authors: Lee, HS;Kwon, YJ;Seo, EB;Kim, SK;Lee, H;Lee, JT;Chang, PS;Choi, YJ;Lee, SH;Ye, SK;
    Journal of ethnopharmacology  2023-06-27
  4. Optineurin deficiency impairs autophagy to cause interferon beta overproduction and increased survival of mice following viral infection
    Authors: Fukushi, M;Ohsawa, R;Okinaka, Y;Oikawa, D;Kiyono, T;Moriwaki, M;Irie, T;Oda, K;Kamei, Y;Tokunaga, F;Sotomaru, Y;Maruyama, H;Kawakami, H;Sakaguchi, T;
    PloS one  2023-06-23
  5. p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation-driven pancreatic cancer
    Authors: Miller, P;Akama-Garren, EH;Owen, RP;Demetriou, C;Carroll, TM;Slee, E;Al Moussawi, K;Ellis, M;Goldin, R;O'Neill, E;Lu, X;
    Cell death and differentiation  2023-06-03
  6. MPC-n (IgG) improves long-term cognitive impairment in the mouse model of repetitive mild traumatic brain injury
    Authors: Zhang, C;Wei, C;Huang, X;Hou, C;Liu, C;Zhang, S;Zhao, Z;Liu, Y;Zhang, R;Zhou, L;Li, Y;Yuan, X;Zhang, J;
    BMC medicine  2023-05-30
  7. Tumour extracellular vesicles and particles induce liver metabolic dysfunction
    Authors: Wang, G;Li, J;Bojmar, L;Chen, H;Li, Z;Tobias, GC;Hu, M;Homan, EA;Lucotti, S;Zhao, F;Posada, V;Oxley, PR;Cioffi, M;Kim, HS;Wang, H;Lauritzen, P;Boudreau, N;Shi, Z;Burd, CE;Zippin, JH;Lo, JC;Pitt, GS;Hernandez, J;Zambirinis, CP;Hollingsworth, MA;Grandgenett, PM;Jain, M;Batra, SK;DiMaio, DJ;Grem, JL;Klute, KA;Trippett, TM;Egeblad, M;Paul, D;Bromberg, J;Kelsen, D;Rajasekhar, VK;Healey, JH;Matei, IR;Jarnagin, WR;Schwartz, RE;Zhang, H;Lyden, D;
    Nature  2023-05-24
  8. Myeloid Cell Derived IL1? Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction
    Authors: Agrawal, V;Kropski, JA;Gokey, JJ;Kobeck, E;Murphy, M;Murray, KT;Fortune, NL;Moore, CS;Meoli, DF;Monahan, K;Su, YR;Blackwell, T;Gupta, DK;Talati, MH;Gladson, S;Carrier, EJ;West, JD;Hemnes, AR;
    bioRxiv : the preprint server for biology  2023-05-18
  9. Progerinin, an Inhibitor of Progerin, Alleviates Cardiac Abnormalities in a Model Mouse of Hutchinson-Gilford Progeria Syndrome
    Authors: Kang, SM;Seo, S;Song, EJ;Kweon, O;Jo, AH;Park, S;Woo, TG;Kim, BH;Oh, GT;Park, BJ;
    Cells  2023-04-24
  10. Irradiation plus myeloid-derived suppressor cell-targeted therapy for overcoming treatment resistance in immunologically cold urothelial carcinoma
    Authors: S Yamamoto, M Kato, Y Takeyama, Y Azuma, N Yukimatsu, Y Hirayama, T Otoshi, T Yamasaki, M Fujioka, M Gi, H Wanibuchi, J Uchida
    British Journal of Cancer, 2023-04-17;0(0):.  2023-04-17
  11. Spatiotemporal Cofilin Signaling, Microglial Activation, Neuroinflammation, and Cognitive Impairment Following Hemorrhagic Brain Injury
    Authors: Almarghalani, DA;Sha, X;Mrak, RE;Shah, ZA;
    Cells  2023-04-13
  12. Astrocytic Slc4a4 regulates blood-brain barrier integrity in healthy and stroke brains via a NO-CCL2-CCR2 pathway
    Authors: Q Ye, J Jo, CY Wang, H Oh, TJ Choy, K Kim, A Dâ Alessan, YK Reshetnyak, SY Jung, Z Chen, SP Marrelli, HK Lee
    bioRxiv : the preprint server for biology, 2023-04-03;0(0):.  2023-04-03
  13. Correction of haemorrhagic shock-associated coagulopathy and impaired haemostasis by plasma, prothrombin complex concentrates or an activated protein C-targeted DNA aptamer in mice
    Authors: LJ Eltringham, SC Meixner, ELG Pryzdial, WP Sheffield
    Scientific Reports, 2023-03-07;13(1):3811.  2023-03-07
  14. CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy
    Authors: X Bai, ZQ Guo, YP Zhang, ZZ Fan, LJ Liu, L Liu, LL Long, SC Ma, J Wang, Y Fang, XR Tang, YJ Zeng, X Pan, DH Wu, ZY Dong
    Nature Communications, 2023-03-04;14(1):1247.  2023-03-04
  15. Polarized Anti-Inflammatory Mesenchymal Stem Cells Increase Hippocampal Neurogenesis and Improve Cognitive Function in Aged Mice
    Authors: M Tfilin, N Gobshtis, D Fozailoff, VE Fraifeld, G Turgeman
    International Journal of Molecular Sciences, 2023-02-24;24(5):.  2023-02-24
  16. Hypoxia alters the immune response in mouse peritoneal macrophages infected with influenza a virus with truncated NS1 protein
    Authors: M Hancková, L Miháliková, S Pastorekov, T Betáková
    Cytokine, 2023-02-14;164(0):156138.  2023-02-14
  17. CD26-negative and CD26-positive tissue-resident fibroblasts contribute to functionally distinct CAF subpopulations in breast cancer
    Authors: JM Houthuijze, R de Bruijn, E van der Bu, AP Drenth, E Wientjens, T Filipovic, E Bullock, CS Brambillas, EM Pulver, M Nieuwland, I de Rink, F van Diepen, S Klarenbeek, R Kerkhoven, VG Brunton, CLGJ Scheele, MC Boelens, J Jonkers
    Nature Communications, 2023-01-12;14(1):183.  2023-01-12
  18. TGFbeta1+CCR5+ neutrophil subset increases in bone marrow and causes age-related osteoporosis in male mice
    Authors: J Li, Z Yao, X Liu, R Duan, X Yi, A Ayoub, JO Sanders, A Mesfin, L Xing, BF Boyce
    Nature Communications, 2023-01-11;14(1):159.  2023-01-11
  19. Disruption of CCL2 in Mesenchymal Stem Cells as an Anti-Tumor Approach against Prostate Cancer
    Authors: QT Bui, KD Lee, YC Fan, BS Lewis, LW Deng, YC Tsai
    Cancers, 2023-01-10;15(2):.  2023-01-10
  20. Radiofrequency Ablation Remodels the Tumor Microenvironment and Promotes Neutrophil-Mediated Abscopal Immunomodulation in Pancreatic Cancer
    Authors: EY Faraoni, BJ O'Brien, LN Strickland, BK Osborn, V Mota, J Chaney, CL Atkins, P Cen, J Rowe, J Cardenas, KL Poulsen, CJ Wray, NC Thosani, JM Bailey-Lun
    Cancer Immunology Research, 2023-01-03;11(1):4-12.  2023-01-03
  21. Regeneration of collecting lymphatic vessels following injury
    Authors: Razavi, M;Lei, P;Amoozgar, Z;Leartprapun, N;Nadkarni, S;Baish, J;Padera, T;Munn, L;
    Research Square  2023-01-01
  22. CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy
    Authors: X Bai, ZQ Guo, YP Zhang, ZZ Fan, LJ Liu, L Liu, LL Long, SC Ma, J Wang, Y Fang, XR Tang, YJ Zeng, X Pan, DH Wu, ZY Dong
    Nature Communications, 2023;14(1):1247.  2023
  23. KLK6/PAR1 Axis Promotes Tumor Growth and Metastasis by Regulating Cross-Talk between Tumor Cells and Macrophages
    Authors: YS Hwang, HJ Cho, ES Park, J Lim, HR Yoon, JT Kim, SR Yoon, H Jung, YK Choe, YH Kim, CH Lee, YT Kwon, BY Kim, HG Lee
    Cells, 2022-12-16;11(24):.  2022-12-16
  24. Intermittent scavenging of storage lesion from stored red blood cells by electrospun nanofibrous sheets enhances their quality and shelf-life
    Authors: S Pandey, M Mahato, P Srinath, U Bhutani, TJ Goap, P Ravipati, PK Vemula
    Nature Communications, 2022-12-01;13(1):7394.  2022-12-01
  25. Neuronal deletion of MnSOD in mice leads to demyelination, inflammation and progressive paralysis that mimics phenotypes associated with progressive multiple sclerosis
    Authors: S Bhaskaran, G Kumar, N Thadathil, KM Piekarz, S Mohammed, SD Lopez, R Qaisar, D Walton, JL Brown, A Murphy, N Smith, D Saunders, MJ Beckstead, S Plafker, TL Lewis, R Towner, SS Deepa, A Richardson, RC Axtell, H Van Remmen
    Redox Biology, 2022-11-26;59(0):102550.  2022-11-26
  26. Oleuropein suppresses endometriosis progression and improves the fertility of mice with endometriosis
    Authors: Y Park, YJ Cho, N Sung, MJ Park, X Guan, WE Gibbons, BW O'Malley, SJ Han
    Journal of Biomedical Science, 2022-11-22;29(1):100.  2022-11-22
  27. Involvement of the p38 MAPK-NLRC4-Caspase-1 Pathway in Ionizing Radiation-Enhanced Macrophage IL-1beta Production
    Authors: JS Baik, YN Seo, YC Lee, JM Yi, MH Rhee, MT Park, SD Kim
    International Journal of Molecular Sciences, 2022-11-09;23(22):.  2022-11-09
  28. Systemic checkpoint blockade by PD-L1 single-chain antibody confers potent anti-tumor immunity and long-term survival
    Authors: H Wang, V Khattar, JA Hensel, R Ashton, Y Lu, AG Sorace, Y Wang, JS Deshane, JL Mieher, C Deivanayag, S Ponnazhaga
    Molecular Cancer Therapeutics, 2022-11-03;0(0):.  2022-11-03
  29. Glutamyl-prolyl-tRNA synthetase 1 coordinates early endosomal anti-inflammatory AKT signaling
    Authors: EY Lee, SM Kim, JH Hwang, SY Jang, S Park, S Choi, GS Lee, J Hwang, JH Moon, PL Fox, S Kim, CH Lee, MH Kim
    Nature Communications, 2022-10-29;13(1):6455.  2022-10-29
  30. AhR Mediated Activation of Pro-Inflammatory Response of RAW 264.7 Cells Modulate the Epithelial-Mesenchymal Transition
    Authors: P Selvam, CM Cheng, HU Dahms, VK Ponnusamy, YY Sun
    Toxics, 2022-10-27;10(11):.  2022-10-27
  31. Oral administration of human carbonic anhydrase I suppresses colitis in a murine inflammatory bowel disease model
    Authors: K Tange, S Yagi, E Takeshita, M Abe, Y Yamamoto, H Tomida, T Kawamura, M Hanayama, B Matsuura, Y Ikeda, Y Hiasa
    Scientific Reports, 2022-10-26;12(1):17983.  2022-10-26
  32. Functional Complementation of Anti-Adipogenic Phytonutrients for Obesity Prevention and Management
    Authors: Y Urasaki, TT Le
    Nutrients, 2022-10-16;14(20):.  2022-10-16
  33. Nanoparticle-based modulation of CD4+ T cell effector and helper functions enhances adoptive immunotherapy
    Authors: A Isser, AB Silver, HC Pruitt, M Mass, EH Elias, G Aihara, SS Kang, N Bachmann, YY Chen, EK Leonard, JG Bieler, W Chaisawang, J Choy, SR Shannon, S Gerecht, JS Weber, JB Spangler, JP Schneck
    Nature Communications, 2022-10-14;13(1):6086.  2022-10-14
  34. OGG1 Inhibition Reduces Acinar Cell Injury in a Mouse Model of Acute Pancreatitis
    Authors: Z Hajnády, M Nagy-Pénze, MA Demény, K Kovács, T El-Hamoly, J Maléth, P Hegyi, Z Polgár, C Heged?s, L Virág
    Biomedicines, 2022-10-12;10(10):.  2022-10-12
  35. Mineralocorticoid receptor antagonists and glucocorticoids differentially affect skeletal muscle inflammation and pathology in muscular dystrophy
    Authors: ZM Howard, CK Gomatam, CP Rabolli, J Lowe, AB Piepho, SS Bansal, F Accornero, JA Rafael-For
    JCI Insight, 2022-10-10;0(0):.  2022-10-10
  36. Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses
    Authors: Q Wang, HY Li, ZM Ling, G Chen, ZY Wei
    Journal of Neuroinflammation, 2022-10-04;19(1):244.  2022-10-04
  37. Arginine methylation of integrin alpha-4 prevents fibrosis development in alcohol-associated liver disease
    Authors: M Schonfeld, MT Villar, A Artigues, SA Weinman, I Tikhanovic
    Cellular and Molecular Gastroenterology and Hepatology, 2022-09-30;0(0):.  2022-09-30
  38. FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation
    Authors: W Cai, M Hu, C Li, R Wu, D Lu, C Xie, W Zhang, T Li, S Shen, H Huang, W Qiu, Q Liu, Y Lu, Z Lu
    Autophagy, 2022-09-28;0(0):1-20.  2022-09-28
  39. NIL10: A New IL10-Receptor Binding Nanoparticle That Induces Cardiac Protection in Mice and Pigs Subjected to Acute Myocardial Infarction through STAT3/NF-kappaB Activation
    Authors: L Tesoro, I Hernández, R Ramírez-Ca, J Díez-Mata, N Alcharani, B Jiménez-Gu, K Ovejero-Pa, M Filice, JL Zamorano, M Saura, C Zaragoza, L Botana
    Pharmaceutics, 2022-09-26;14(10):.  2022-09-26
  40. SH005S7 Overcomes Primary and Acquired Resistance of Non-Small Cell Lung Cancer by Combined MET/EGFR/HER3 Inhibition
    Authors: S Kang, JH Kim, J Hong, JH Moon, YY Kwon, SG Ko
    BioMed Research International, 2022-09-15;2022(0):1840541.  2022-09-15
  41. Neutrophil-mediated fibroblast-tumor cell il-6/stat-3 signaling underlies the association between neutrophil-to-lymphocyte ratio dynamics and chemotherapy response in localized pancreatic cancer: A hybrid clinical-preclinical study
    Authors: I de Castro, A Bianchi, NU Deshpande, P Sharma, S Mehra, VT Garrido, SJ Saigh, J England, PJ Hosein, D Kwon, NB Merchant, J Datta
    Elife, 2022-09-15;11(0):.  2022-09-15
  42. CD8+ T-cell responses are boosted by dual PD-1/VEGFR2 blockade after EGFR inhibition in Egfr-mutant lung cancer
    Authors: K Nishii, K Ohashi, S Tomida, T Nakasuka, A Hirabae, S Okawa, J Nishimura, H Higo, H Watanabe, H Kano, C Ando, G Makimoto, K Ninomiya, Y Kato, T Kubo, E Ichihara, K Hotta, M Tabata, S Toyooka, H Udono, Y Maeda, K Kiura
    Cancer Immunology Research, 2022-09-01;0(0):.  2022-09-01
  43. Differential progression of unhealthy diet-induced hepatocellular carcinoma in obese and non-obese mice
    Authors: E Hymel, E Vlock, KW Fisher, PA Farazi
    PLoS ONE, 2022-08-22;17(8):e0272623.  2022-08-22
  44. Macrophage-Specific, Mafb-Deficient Mice Showed Delayed Skin Wound Healing
    Authors: Y Inoue, CW Liao, Y Tsunakawa, IL Tsai, S Takahashi, M Hamada
    International Journal of Molecular Sciences, 2022-08-19;23(16):.  2022-08-19
  45. Maternal soybean diet on prevention of obesity-related breast cancer through early-life gut microbiome and epigenetic regulation: Maternal soybean prevention on obesity-related breast cancer
    Authors: M Chen, S Li, I Arora, N Yi, M Sharma, Z Li, TO Tollefsbol, Y Li
    The Journal of nutritional biochemistry, 2022-08-03;0(0):109119.  2022-08-03
  46. Naegleria fowleri Cathepsin B Induces a Pro-Inflammatory Immune Response in BV-2 Microglial Cells via NF-kappaB and AP-1 Dependent-MAPK Signaling Pathway
    Authors: HG Lê, JM Kang, TC Võ, BK Na
    International Journal of Molecular Sciences, 2022-07-29;23(15):.  2022-07-29
  47. Sulfaphenazole reduces thermal and�pressure injury severity through rapid restoration of tissue perfusion
    Authors: CT Turner, M Pawluk, J Bolsoni, MR Zeglinski, Y Shen, H Zhao, T Ponomarev, KC Richardson, CR West, A Papp, DJ Granville
    Scientific Reports, 2022-07-23;12(1):12622.  2022-07-23
  48. Synthetic Essentiality of Tryptophan 2,3-dioxygenase 2 in APC-Mutated Colorectal Cancer
    Authors: R Lee, J Li, J Li, CJ Wu, S Jiang, WH Hsu, D Chakravart, P Chen, KA LaBella, J Li, DJ Spring, D Zhao, YA Wang, RA DePinho
    Cancer Discovery, 2022-07-06;0(0):.  2022-07-06
  49. Spontaneous Myocarditis in Mice Predisposed to Autoimmune Disease: Including Vaccination-Induced Onset
    Authors: T Hayashi, M Ichikawa, I Konishi
    Biomedicines, 2022-06-18;10(6):.  2022-06-18
  50. Brain motor and fear circuits regulate leukocytes during acute stress
    Authors: WC Poller, J Downey, AA Mooslechne, N Khan, L Li, CT Chan, CS McAlpine, C Xu, F Kahles, S He, H Janssen, JE Mindur, S Singh, MG Kiss, L Alonso-Her, Y Iwamoto, RH Kohler, LP Wong, K Chetal, SJ Russo, RI Sadreyev, R Weissleder, M Nahrendorf, PS Frenette, M Divangahi, FK Swirski
    Nature, 2022-05-30;0(0):.  2022-05-30
  51. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions
    Authors: L Brandolini, M d'Angelo, R Novelli, V Castelli, C Giorgio, A Sirico, P Cocchiaro, F D'Egidio, E Benedetti, C Cristiano, A Bugatti, A Ruocco, PG Amendola, C Talarico, C Manelfi, D Iaconis, A Beccari, AU Quadros, TM Cunha, A Caruso, R Russo, A Cimini, A Aramini, M Allegretti
    Cell Death & Disease, 2022-05-25;13(5):500.  2022-05-25
  52. Mechanisms Underlying Antipsychotic-Induced NAFLD and Iron Dysregulation: A Multi-Omic Approach
    Authors: M May, D Barlow, R Ibrahim, KL Houseknech
    Oncogene, 2022-05-24;10(6):.  2022-05-24
  53. Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity
    Authors: TN Pham, C Spaulding, MA Shields, AE Metropulos, DN Shah, MG Khalafalla, DR Principe, DJ Bentrem, HG Munshi
    JCI Insight, 2022-05-09;0(0):.  2022-05-09
  54. Mesenchymal stem cells empower T cells in the lymph nodes via MCP-1/PD-L1 axis
    Authors: Y He, Y Qu, B Meng, W Huang, J Tang, R Wang, Z Chen, X Kou, S Shi
    Cell Death & Disease, 2022-04-18;13(4):365.  2022-04-18
  55. Presenilin 2 N141I mutation induces hyperactive immune response through the epigenetic repression of REV-ERBalpha
    Authors: H Nam, Y Lee, B Kim, JW Lee, S Hwang, HK An, KM Chung, Y Park, J Hong, K Kim, EK Kim, HK Choe, SW Yu
    Nature Communications, 2022-04-13;13(1):1972.  2022-04-13
  56. Recombinant Klotho Protein Ameliorates Myocardial Ischemia/Reperfusion Injury by Attenuating Sterile Inflammation
    Authors: J Myung, JH Beom, JH Kim, JS Woo, I Park, SP Chung, YE Chung, JS You
    Biomedicines, 2022-04-13;10(4):.  2022-04-13
  57. Survival of stem cells and progenitors in the intestine is regulated by LPA5-dependent signaling
    Authors: Z Liang, P He, Y Han, CC Yun
    Cellular and Molecular Gastroenterology and Hepatology, 2022-04-04;0(0):.  2022-04-04
  58. Modulation of the cell membrane lipid milieu by peroxisomal beta-oxidation induces Rho1 signaling to trigger inflammatory responses
    Authors: AS Nath, BD Parsons, S Makdissi, RL Chilvers, Y Mu, CM Weaver, I Euodia, KA Fitze, J Long, M Scur, DP Mackenzie, AP Makrigiann, N Pichaud, LH Boudreau, AJ Simmonds, CA Webber, B Derfalvi, Y Hammon, RA Rachubinsk, F Di Cara
    Cell Reports, 2022-03-01;38(9):110433.  2022-03-01
  59. P62/SQSTM1 enhances osteogenesis and attenuates inflammatory signals in bone marrow microenvironment
    Authors: D Agas, V Gabai, AA Sufianov, A Shneider, M Giovanna S
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    Am J Pathol, 2013-03-05;182(5):1740-9.  2013-03-05
  311. Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation.
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  312. The MSHA Strain of Pseudomonas Aeruginosa Activated TLR Pathway and Enhanced HIV-1 DNA Vaccine Immunoreactivity.
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  313. Interleukin-33 primes mast cells for activation by IgG immune complexes.
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  314. NLRP3 suppresses NK cell-mediated responses to carcinogen-induced tumors and metastases.
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    Cancer Res, 2012-09-17;72(22):5721-32.  2012-09-17
  315. TGFbeta signalling plays an important role in IL4-induced alternative activation of microglia.
    J Neuroinflammation, 2012-09-04;9(0):210.  2012-09-04
  316. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
    J. Immunol., 2012-07-30;189(5):2545-52.  2012-07-30
  317. Exosomes isolated from mycobacteria-infected mice or cultured macrophages can recruit and activate immune cells in vitro and in vivo.
    Authors: Singh PP, Smith VL, Karakousis PC, Schorey JS
    J. Immunol., 2012-06-20;189(2):777-85.  2012-06-20
  318. Macrophage Dectin-1 Expression Is Controlled by Leukotriene B4 via a GM-CSF/PU.1 Axis.
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    J. Immunol., 2012-06-13;189(2):906-15.  2012-06-13
  319. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
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    Mucosal Immunol, 2012-06-13;5(0):702.  2012-06-13
  320. A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
    Acta Pharmacol. Sin., 2012-06-11;33(9):1204-16.  2012-06-11
  321. Histone Deacetylase 3 Mediates Allergic Skin Inflammation by Regulating Expression of MCP1 Protein.
    Authors: Kim Y, Kim K, Park D, Lee E, Lee H, Lee YS, Choe J, Jeoung D
    J. Biol. Chem., 2012-06-07;287(31):25844-59.  2012-06-07
  322. Abcg2 deficiency augments oxidative stress and cognitive deficits in Tg-SwDI transgenic mice.
    J. Neurochem., 2012-06-06;233(0):456-469.  2012-06-06
  323. Pten loss in CD4 T cells enhances their helper function but does not lead to autoimmunity or lymphoma.
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    J. Immunol., 2012-05-18;188(12):5935-43.  2012-05-18
  324. Early-outgrowth bone marrow cells attenuate renal injury and dysfunction via an antioxidant effect in a mouse model of type 2 diabetes.
    Diabetes, 2012-05-17;61(8):2114-25.  2012-05-17
  325. Clusterin induces the secretion of TNF-α and the chemotactic migration of macrophages.
    Authors: Shim YJ, Kang BH, Choi BK, Park IS, Min BH
    Biochem. Biophys. Res. Commun., 2012-05-07;422(1):200-5.  2012-05-07
  326. Dietary blue pigments derived from genipin, attenuate inflammation by inhibiting LPS-induced iNOS and COX-2 expression via the NF-kappaB inactivation.
    Authors: Wang QS, Xiang Y, Cui YL, Lin KM, Zhang XF
    PLoS ONE, 2012-03-30;7(3):e34122.  2012-03-30
  327. A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.
    Authors: Wang H, Beyer I, Persson J, Song H, Li Z, Richter M, Cao H, van Rensburg R, Yao X, Hudkins K, Yumul R, Zhang XB, Yu M, Fender P, Hemminki A, Lieber A
    J. Virol., 2012-03-28;86(11):6286-302.  2012-03-28
  328. The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
    Authors: Tran K, Risingsong R, Royce D
    Cancer Prev Res (Phila), 2012-03-08;5(5):726-34.  2012-03-08
  329. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
    Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
    PLoS ONE, 2012-02-29;7(2):e32125.  2012-02-29
  330. Preventing phosphorylation of sterol regulatory element-binding protein 1a by MAP-kinases protects mice from fatty liver and visceral obesity.
    Authors: Kotzka J, Knebel B, Haas J, Kremer L, Jacob S, Hartwig S, Nitzgen U, Muller-Wieland D
    PLoS ONE, 2012-02-27;7(2):e32609.  2012-02-27
  331. Liver-specific expression of transcriptionally active SREBP-1c is associated with fatty liver and increased visceral fat mass.
    Authors: Knebel B, Haas J, Hartwig S, Jacob S, Kollmer C, Nitzgen U, Muller-Wieland D, Kotzka J
    PLoS ONE, 2012-02-21;7(2):e31812.  2012-02-21
  332. The effect of oxidized low density lipoprotein (oxLDL)-induced heme oxygenase-1 on LPS-induced inflammation in RAW 264.7 macrophage cells.
    Authors: Min KJ, Cho KH, Kwon TK
    Cell. Signal., 2012-02-10;24(6):1215-21.  2012-02-10
  333. Identification and functional characterization of novel phosphorylation sites in TAK1-binding protein (TAB) 1.
    Authors: Wolf A, Beuerlein K, Eckart C, Weiser H, Dickkopf B, Muller H, Sakurai H, Kracht M
    PLoS ONE, 2011-12-22;6(12):e29256.  2011-12-22
  334. Murine bone marrow-derived macrophages differentiated with GM-CSF become foam cells by PI3Kgamma-dependent fluid-phase pinocytosis of native LDL.
    Authors: Anzinger JJ, Chang J, Xu Q, Barthwal MK, Bohnacker T, Wymann MP, Kruth HS
    J. Lipid Res., 2011-11-04;53(1):34-42.  2011-11-04
  335. Stromal interaction essential for vascular endothelial growth factor A-induced tumour growth via transforming growth factor-beta signalling.
    Authors: Weidenaar AC, Ter Elst A, Kampen KR, Meeuwsen-de Boer TG, de Jonge HJ, Scherpen FJ, den Dunnen WF, Kamps WA, de Bont ES
    Br. J. Cancer, 2011-11-01;105(12):1856-63.  2011-11-01
  336. Attenuation of leukocyte recruitment via CXCR1/2 inhibition stops the progression of PAH in mice with genetic ablation of endothelial BMPR-II.
    Authors: Burton VJ, Holmes AM, Ciuclan LI
    Blood, 2011-09-07;118(17):4750-8.  2011-09-07
  337. Inhibition of IL-32 activation by alpha-1 antitrypsin suppresses alloreactivity and increases survival in an allogeneic murine marrow transplantation model.
    Authors: Marcondes AM, Li X, Tabellini L, Bartenstein M, Kabacka J, Sale GE, Hansen JA, Dinarello CA, Deeg HJ
    Blood, 2011-09-06;118(18):5031-9.  2011-09-06
  338. Fine-tuning nucleophosmin in macrophage differentiation and activation.
    Authors: Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, Jacquel A, Cally R, Manoury B, Vanden Berghe T, Vandenabeele P, Droin N, Solary E
    Blood, 2011-08-29;118(17):4694-704.  2011-08-29
  339. Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates Escherichia coli-induced acute lung injury in mice.
    Authors: Kim ES, Chang YS, Choi SJ, Kim JK, Yoo HS, Ahn SY, Sung DK, Kim SY, Park YR, Park WS
    Respir. Res., 2011-08-15;12(0):108.  2011-08-15
  340. Comparative kinetic analyses of gene profiles of naive CD4+ and CD8+ T cells from young and old animals reveal novel age-related alterations.
    Authors: Mirza N, Pollock K, Hoelzinger DB, Dominguez AL, Lustgarten J
    Aging Cell, 2011-08-07;10(5):853-67.  2011-08-07
  341. Immuno-therapy with anti-CTLA4 antibodies in tolerized and non-tolerized mouse tumor models.
    Authors: Persson J, Beyer I, Yumul R, Li Z, Kiem HP, Roffler S, Lieber A
    PLoS ONE, 2011-07-14;6(7):e22303.  2011-07-14
  342. TGF-beta limits IL-33 production and promotes the resolution of colitis through regulation of macrophage function.
    Authors: Rani R, Smulian AG, Greaves DR, Hogan SP, Herbert DR
    Eur. J. Immunol., 2011-05-27;41(7):2000-9.  2011-05-27
  343. Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation.
    Authors: Koroskenyi K, Duro E, Pallai A, Sarang Z, Kloor D, Ucker DS, Beceiro S, Castrillo A, Chawla A, Ledent CA, Fésüs L, Szondy Z
    J. Immunol., 2011-05-18;186(12):7144-55.  2011-05-18
  344. SHIP-deficient mice develop spontaneous intestinal inflammation and arginase-dependent fibrosis.
    Authors: McLarren KW, Cole AE, Weisser SB, Voglmaier NS, Conlin VS, Jacobson K, Popescu O, Boucher JL, Sly LM
    Am. J. Pathol., 2011-05-07;179(1):180-8.  2011-05-07
  345. TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
    Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
    J. Lipid Res., 2011-04-26;52(7):1307-18.  2011-04-26
  346. Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
    Authors: Diao J, Zhao J, Winter E
    J. Immunol., 2011-03-23;186(9):5058-67.  2011-03-23
  347. Reduced VEGF production, angiogenesis, and vascular regrowth contribute to the antitumor properties of dual mTORC1/mTORC2 inhibitors.
    Authors: Falcon BL, Barr S, Gokhale PC, Chou J, Fogarty J, Depeille P, Miglarese M, Epstein DM, McDonald DM
    Cancer Res., 2011-03-01;71(5):1573-83.  2011-03-01
  348. HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation.
    Authors: Braunstein M, Anderson MK
    Mol. Cell. Biol., 2010-12-28;31(5):971-82.  2010-12-28
  349. Caspase-3 is transiently activated without cell death during early antigen driven expansion of CD8(+) T cells in vivo.
    Authors: McComb S, Mulligan R, Sad S
    PLoS ONE, 2010-12-22;5(12):e15328.  2010-12-22
  350. A CD36-dependent pathway enhances macrophage and adipose tissue inflammation and impairs insulin signalling.
    Authors: Kennedy DJ, Kuchibhotla S
    Cardiovasc. Res., 2010-11-18;0(0):.  2010-11-18
  351. Tim3 binding to galectin-9 stimulates antimicrobial immunity.
    Authors: Jayaraman P, Sada-Ovalle I, Beladi S, Anderson AC, Dardalhon V, Hotta C, Kuchroo VK, Behar SM
    J. Exp. Med., 2010-10-11;207(11):2343-54.  2010-10-11
  352. Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
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    J. Immunol., 2010-09-15;185(8):4659-72.  2010-09-15
  353. Salmonella enterica serovar Typhimurium-induced placental inflammation and not bacterial burden correlates with pathology and fatal maternal disease.
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  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

View all Proteome Profiler Antibody Array FAQs

Reviews for Proteome Profiler Mouse Cytokine Array Kit, Panel A

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Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Khuynh Nguyen on 08/15/2023

The kit works very well

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/25/2023

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Andres Ocampo on 10/11/2022

Mouse Cytokine Antibody Array was used in serum from Prolactin Receptor wild type baby mice after the mother's high-fat diet feeding for 21 days of lactation, and results were compared with Control Diet (CD). A low-level pro-inflammatory mechanism could be confirmed due to the modulation of inflammation-associated cytokines.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/01/2022

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Martin Torres on 10/01/2021

The array was used to test the anti-inflammatory potential from Octopus vulgaris ink extracts on LPS-stimulated RAW 264.7 cells. Results obtained were in agreement with other biochemical and in vitro test. We have used this kit in several LPS-challenged murine macrophages. Fig. A shows the expression level of each cytokine against the control (LPS only-treated macrophages); B shows a bioinformatic arrangement of the modulated cytokines, and C shows a predictive analysis from the results obtained in this kit.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Martin Torres on 09/23/2021

Pro and anti-inflammatory cytokines were evaluated in RAW264.7 macrophages treated with LPS (1 microgram/mL) and a South American berry (ABJ). Results obtained showed the modulation of several cytokines indicated in this kit. We have conducted several evaluations of these cytokines using this kit and results never disappoint. What we like the most is that this assay allows the evaluation of several cytokines that would take a lot of time by conventional techniques such as ELISA or Western Blot.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/23/2021

20 ul serum was used to perform the experiment.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 08/31/2021

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anil Singh on 08/25/2021

mouse serum 200ul used for cancer model

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Gaurav Kumar on 08/17/2021

Used serum to see the role of gene in pavreatic cancer

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Allison Scarpitti on 05/10/2021

Phenomenal results, consistent performance and excellent translation to use with Streptavidin-800. On step 11, I swapped the Streptavidin-HRP for a 1:2,000 ratio of Streptavidin-800. Then followed with the wash steps and the result is as posted.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/06/2021

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By jinxiu PAN on 02/24/2020


Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020

mouse tissues

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020

serum from knock-out mice

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/08/2020

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/10/2019

Followed protocol exactly, 10 min exposure on film wasn't great, boosted to 20 min exposure, still not good. 5 min on digital imager and positive control spots were saturated! Much more sensitive!

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 06/06/2019

T cells isolated and stimulated with various agonists.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/05/2019


Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Ivan Luzardo on 03/13/2019

This array was used to measure the modulation of inflammation-associated cytokines in LPS-stimulated RAW264.7 macrophages treated with several doses of the non-digestible fraction from a corn/bean snack.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Brian Englar on 02/07/2019

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/26/2018

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Ivan Luzardo on 12/11/2018

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 11/11/2018

High quality results with simple and straightforward protocol. No optimization needed.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/19/2018

instructions easy to follow. I needed a little longer than the 10 minute exposure due to low protein concentrations from my very small tissues.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/26/2018

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Adam Guess on 10/20/2017

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/27/2017

Very sensitive cytokine assay!

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/14/2017

performed on cell conditoned media from mouse fibroblasts

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Michelle Chen on 05/10/2017

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Camila Idelí Morales Fénero on 03/15/2017

Assay tested in 200 ug of protein from pool of intestines of 10 dpf zebrafish larvae.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/27/2017

Mouse BALF samples from pooled control and treatment groups in 2 separate arrays to determine the altered cytokine response.

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 01/27/2017

Bronchoalveolar lavage fluid from C57BL/6J mouse

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/06/2016

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Kihong Lim on 09/29/2016