R&D Systems Human Cytokine Array, Panel A (Catalog # ARY005) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 36 different cytokines, chemokines, and acute phase proteins in a single sample. No specialized equipment is necessary.
|Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.|
|CCL1/I-309||IL-12 p70||CCL3/MIP-1 alpha|
|IL-1 beta||IL-17E||Serpin E1/PAI-1|
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.