Antibodies for Multiplex Immunofluorescence (mIF)

Multiplexed immunofluorescence (mIF) is a powerful technique for investigating complex biological systems and cellular interactions. It allows researchers to simultaneously detect multiple targets within a single tissue, providing a deeper understanding of cellular dynamics, spatial relationships, and biomarker expression patterns.

Table of Contents

• Featured COMET Qualified Antibodies
• Qualified Antibodies Table
• Qualification Process on COMET
• Other Spatial Tested Antibodies

Multiplex Immunofluorescence (mIF) on COMET™

The COMET™ platform, from Lunaphore, a Bio-Techne brand, is a fully-automated, high-throughput, spatial biology platform that performs sequential immunofluorescence (seqIF™) through staining, imaging, and elution cycles. It offers the unique flexibility to work with off-the-shelf, non-conjugated primary antibodies. 

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Featured Multiplex IF Antibodies Qualified on COMET™

COMET™ Qualified mIF Antibodies

Table 1: List of COMET™ Qualified mIF Antibodies from R&D Systems™ and Novus Biologicals™ part of Bio-Techne.

Antibody Testing Process on COMET™

Bio-Techne's antibody validation experts have collaborated and trained alongside Lunaphore's research and development team covering protocols and requirements involved in the antibody validation process on COMET™. This includes the antibody qualification process for protocol optimization, with a focus on assessing specificity, sensitivity, and elution efficiency using positive control tissue. At Bio-Techne, our IHC validated antibodies have already undergone a rigorous process to be added to the catalog.  Antibody qualification process for COMET™ involves several key aspects as shown in Figure 1.

Figure 4. Steps of Antibody Testing Process on COMET™ that leads to Lunaphore Certified badge

Multiplex IF (mIF) validated antibodies with a Lunaphore certified badge are taken through the qualification or verification process listed above. This rigorous testing process ensures that antibodies, from R&D Systems™ and Novus Biologicals™, part of Bio-Techne, deliver high-quality and specific staining on the COMET™ platform.  Bio-Techne Lunaphore Certified antibodies are also in the Lunaphore Panel Builder and added to the expanding COMET^™ marker database.