Advancing the Culture and Clonogenic Analysis of Gastrointestinal Stem Cells

Tuesday, June 19, 2018 - 09:19

In this webinar, Drs. Wa Xian and Frank McKeon, detail their work advancing model systems of the gastrointestinal tract. Specifically, their research focuses on utilizing a powerful system for the isolation, clonogenic culture, and differentiation of adult gastrointestinal stem cells, as described in Wang, X. et al (2015) Nature 522:173. They discuss why their system is vastly more simple and robust than the induced pluripotent stem cell (iPSC) and organoid platforms that presently dominate gastrointestinal research, how it is the only system that enables the dissection of stem cell heterogeneity underlying human disease, and why it is ideal for genome editing, autologous transplantation, large scale pathway analysis, and disease modeling. Their research is the foundation of MimEX GI, a 3-D cell culture system offered by R&D Systems.

Topics covered in the webinar:

  • Isolation of “ground-state” gastrointestinal stem cells
  • Their 200-fold proliferative advantage over organoids
  • Regional epigenetic commitment of gastrointestinal stem cells
  • Clonal accessibility for genome editing and autologous transplantation
  • Other advantages over both iPSC and organoid systems
  • Overview of MimEX GI reagents and media

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