The Human Apoptosis Array (Catalog # ARY009) is a rapid, sensitive, and economical tool to simultaneously detect the relative expression of 35 apoptosis-related proteins in a single sample. No specialized equipment is necessary.
|Simultaneously detect the relative levels of these apoptosis-related proteins in a single sample.|
|Pro-Caspase-3||HIF-1 alpha||Phospho-p53 (S46)|
|Cleaved Caspase-3||HO-1/HMOX1/HSP32||Phospho-p53 (S392)|
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cellular extracts are diluted and incubated with the Human Apoptosis Array overnight. The array is washed to remove unbound proteins, followed by incubation with a cocktail of biotinylated detection antibodies. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.