Antibodies for Multiplex Immunofluorescence (mIF)

Multiplexed immunofluorescence (mIF) is a powerful technique for investigating complex biological systems and cellular interactions. It allows researchers to simultaneously detect multiple targets within a single tissue, providing a deeper understanding of cellular dynamics, spatial relationships, and biomarker expression patterns.

Table of Contents

• Featured COMET Qualified Antibodies
• Qualified Antibodies Table
• Qualification Process on COMET
• Other Spatial Tested Antibodies

Multiplex Immunofluorescence (mIF) on COMET™

The COMET platform from Lunaphore, is a fully-automated, high-throughput, spatial biology platform that performs sequential immunofluorescence (seqIF™) through staining, imaging, and elution cycles. It offers the unique flexibility to work with off-the-shelf, non-conjugated primary antibodies. 

COMET Qualified mIF Antibodies

Table 1. List of COMET Qualified Antibodies.

Antibody Testing Process on COMET

The antibody validation process on COMET includes the antibody qualification process for protocol optimization, with a focus on assessing specificity, sensitivity, and elution efficiency using positive control tissue. R&D Systems™, by Bio-Techne, IHC validated antibodies have already undergone a rigorous process to be added to the catalog.  Antibody qualification process for COMET involves several key aspects as shown in Figure 4.

Figure 4. Steps of Antibody Testing Process on COMET.

Multiplex IF (mIF) validated antibodies with a COMET application validation are taken through the qualification or verification process listed above. This rigorous testing process R&D Systems and Novus antibodies deliver high-quality and specific staining on the COMET platform.  R&D Systems COMET tested antibodies are also in the Panel Builder and added to the expanding COMET marker database.