Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
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Proteome Profiler Mouse XL Cytokine Array
R&D Systems | Catalog # ARY028
Contains 4 membranes-each spotted in duplicate with 111 different cytokine antibodies
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Key Product Details
Species
Mouse
Product Summary for Proteome Profiler Mouse XL Cytokine Array
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine.
Troubleshooting GuideKey Benefits
- Detects 111 mouse cytokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Mouse XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
| Simultaneously detect the levels of these cytokines, chemokines, and growth factors in a single sample. | ||
|---|---|---|
| Adiponectin/Acrp30 | DPPIV/CD26 | IL-27 |
| Amphiregulin | EGF | IL-28 |
| Angiopoietin-1 | Endoglin/CD105 | IL-33 |
| Angiopoietin-2 | Endostatin | LDL R |
| Angiopoietin-like 3 | Fetuin A/AHSG | Leptin |
| BAFF/BLyS/TNFSF13B | FGF acidic | LIF |
| C1q R1/CD93 | FGF-21 | Lipocalin-2/NGAL, |
| CCL2/JE/MCP-1 | Flt-3 Ligand | LIX |
| CCL3/CCL4 MIP-1 alpha/beta | Gas6 | M-CSF |
| CCL5/RANTES | G-CSF | MMP-2 |
| CCL6/C10 | GDF-15 | MMP-3 |
| CCL11/Eotaxin | GM-CSF | MMP-9 |
| CCL12/MCP-5 | HGF | Myeloperoxidase |
| CCL17/TARC | ICAM-1/CD54 | Osteopontin (OPN) |
| CCL19/MIP-3 beta | IFN-gamma | Osteoprotegerin/TNFRSF11B |
| CCL20/MIP-3 alpha | IGFBP-1 | PD-ECGF/Thymidine phosphorylase |
| CCL21/6Ckine | IGFBP-2 | PDGF-BB |
| CCL22/MDC | IGFBP-3 | Pentraxin 2/SAP |
| CD14 | IGFBP-5 | Pentraxin 3/ TSG-14 |
| CD40/TNFRSF5 | IGFBP-6 | Periostin/OSF-2 |
| CD160 | IL-1 alpha/IL1F1 | Pref-1/DLK-1/FA1 |
| Chemerin | IL-1 beta/IL-1F2 | Proliferin |
| Chitinase 3-like 1 | IL-1ra/IL-1F3 | Proprotein Convertase 9/PCSK9 |
| Coagulation Factor III/Tissue Factor | IL-2 | RAGE |
| Complement Component C5/C5a | IL-3 | RBP4 |
| Complement Factor D | IL-4 | Reg3G |
| C-Reactive Protein/CRP | IL-5 | Resistin |
| CX3CL1/Fractalkine | IL-6 | E-Selectin/CD62E |
| CXCL1/KC | IL-7 | P-Selectin/CD62P |
| CXCL2/MIP-2 | IL-10 | Serpin E1/PAI-1 |
| CXCL9/MIG | IL-11 | Serpin F1/PEDF |
| CXCL10/IP-10 | IL-12p40 | Thrombopoietin |
| CXCL11/I-TAC | IL-13 | TIM-1/KIM-1/HAVCR |
| CXCL13/BLC/BCA-1 | IL-15 | TNF-alpha |
| CXCL16 | IL-17A | VCAM-1/CD106 |
| Cystatin C | IL-22 | VEGF |
| Dkk-1 | IL-23 | WISP-1/CCN4 |
Assays for Analytes represented in the Mouse XL Cytokine Array Kit
| Analyte | Qkit | Duoset | ||
|---|---|---|---|---|
| Adiponectin/Acrp30 | MRP300 | DY1119 | ||
| Amphiregulin | DY989 | |||
| Angiopoietin-1 | ||||
| Angiopoietin-2 | ||||
| Angiopoietin-like 3 | MANL30 | DY136 | ||
| BAFF/BLyS/TNFSF13B | MBLYS0 | DY2106-05 | ||
| C1q R1/CD93 | MCD930 | |||
| CCL2/JE/MCP-1 | MJE00 | DY479 | ||
| CCL3/CCL4 MIP-1 alpha/beta | ||||
| CCL5/RANTES | MMR00 | DY478 | ||
| CCL6/C10 | DY487 | |||
| CCL11/Eotaxin | MME00 | DY420 | ||
| CCL12/MCP-5 | MCC120 | DY428 | ||
| CCL17/TARC | MCC170 | DY529 | ||
| CCL19/MIP-3 beta | DY440 | |||
| CCL20/MIP-3 alpha | MCC200 | DY760 | ||
| CCL21/6Ckine | DY457 | |||
| CCL22/MDC | MCC220 | DY439 | ||
| CD14 | MC140 | DY982 | ||
| CD40/TNFRSF5 | MCCD40 | DY097 | ||
| CD160 | MCHM00 | DY2325 | ||
| Chemerin | ||||
| Chitinase 3-like 1 | MC3L10 | DY2649 | ||
| Coagulation Factor III/Tissue Factor | ||||
| Complement Component C5/C5a | ||||
| Complement Factor D | ||||
| C-Reactive Protein/CRP | MCRP00 | DY1829 | ||
| CX3CL1/Fractalkine | MCX310 | DY472 | ||
| CXCL1/KC | MKC00B | DY453 | ||
| CXCL2/MIP-2 | MM200 | DY452 | ||
| CXCL9/MIG | MCX900 | DY492 | ||
| CXCL10/IP-10 | MCX100 | DY466 | ||
| CXCL11/I-TAC | DY572 | |||
| CXCL13/BLC/BCA-1 | MCX130 | |||
| CXCL16 | DY503 | |||
| Cystatin C | MSCTC0 | DY1238 | ||
| Dkk-1 | MKK100 | DY1765 | ||
| DPPIV/CD26 | DY954 | |||
| EGF | MEG00 | DY2028 | ||
| Endoglin/CD105 | MNDG00 | DY1320 | ||
| Endostatin | ||||
| Fetuin A/AHSG | MFTA00 | DY1563 | ||
| FGF acidic | DY4686-05 | |||
| FGF-21 | MF2100 | DY3057 | ||
| Flt-3 Ligand | MFK00 | DY427 | ||
| Gas6 | MGAS60 | DY986 | ||
| G-CSF | MCS00 | DY414 | ||
| GDF-15 | MGD150 | DY6385 | ||
| GM-CSF | MGM00 | DY415 | ||
| HGF | MHG00 | DY2207 | ||
| ICAM-1/CD54 | MIC100 | DY796 | ||
| IFN-gamma | MIF00 | DY485 | ||
| IGFBP-1 | DY1588-05 | |||
| IGFBP-2 | DY797 | |||
| IGFBP-3 | MGB300 | DY775 | ||
| IGFBP-5 | DY578 | |||
| IGFBP-6 | DY776 | |||
| IL-1 alpha/IL1F1 | MLA00 | DY400 | ||
| IL-1 beta/IL-1F2 | MLB00C | DY401 | ||
| IL-1ra/IL-1F3 | MRA00 | DY480 | ||
| IL-2 | M2000 | DY402 | ||
| IL-3 | M3000 | DY403 | ||
| IL-4 | M4000B | DY404 | ||
| IL-5 | M5000 | DY405 | ||
| IL-6 | M6000B | DY406 | ||
| IL-7 | M7000 | DY407 | ||
| IL-10 | M1000B | DY417 | ||
| IL-11 | DY418 | |||
| IL-12p40 | MP400 non-allele specific |
M1240 allele specific |
DY2398 non-allele specific |
DY499 allele specific |
| IL-13 | M1300CB | DY413 | ||
| IL-15 | DY447 | |||
| IL-17A | M1700 | DY421 | ||
| IL-22 | M2200 | DY582 | ||
| IL-23 | M2300 | DY1887 | ||
| IL-27 | DY1789B | |||
| IL-28 | ||||
| IL-33 | M3300 | DY3626 | ||
| LDL R | MLDLR0 | DY2255 | ||
| Leptin | MOB00 | DY498 | ||
| LIF | MLF00 | |||
| Lipocalin-2/NGAL, | DY1857 | |||
| LIX | MX000 | DY443 | ||
| M-CSF | MMC00 | DY416 | ||
| MMP-2 | MMP200 | |||
| MMP-3 | MMP300 | DY548 | ||
| MMP-9 | MMPT90 | MMP900B | DY6718 | DY909 |
| Myeloperoxidase | DY3667 | |||
| Osteopontin (OPN) | MOST00 | DY441 | ||
| Osteoprotegerin/TNFRSF11B | MOP00 | DY459 | ||
| PD-ECGF/Thymidine phosphorylase | ||||
| PDGF-BB | MBB00 | |||
| Pentraxin 2/SAP | MPTX20 | |||
| Pentraxin 3/ TSG-14 | MPTX30 | DY2166 | ||
| Periostin/OSF-2 | MOSF20 | DY2955 | ||
| Pref-1/DLK-1/FA1 | ||||
| Proliferin | ||||
| Proprotein Convertase 9/PCSK9 | MPC900 | DY3985 | ||
| RAGE | MRG00 | DY1179 | ||
| RBP4 | MRBP40 | DY3476 | ||
| Reg3G | ||||
| Resistin | MRSN00 | DY1069 | ||
| E-Selectin/CD62E | MES00 | DY575 | ||
| P-Selectin/CD62P | MPS00 | DY737 | ||
| Serpin E1/PAI-1 | DY3828-05 | |||
| Serpin F1/PEDF | ||||
| Thrombopoietin | MTP00 | DY488 | ||
| TIM-1/KIM-1/HAVCR | MKM100 | DY1817 | ||
| TNF-alpha | MTA00B | DY410 | ||
| VCAM-1/CD106 | MVC00 | DY643 | ||
| VEGF | MMV00 | DY493 | ||
| WISP-1/CCN4 | MWSP10 | |||
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Scientific Data Images for Proteome Profiler Mouse XL Cytokine Array
Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Lysate.
Lysates from Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (200 µg lysate, 10 minute exposure).
Detection of Selected Mouse Cytokines in CMT-93 Cell Line Lysate.
Lysates from CMT-93 mouse rectal carcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (200 µg lysate, 10 minute exposure).
Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate.
Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (500 µL of cell culture supernate, 5 minute exposure).
Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Multiplex Cytokine Assay Kits
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Proteome Profiler Mouse XL Cytokine Array
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Proteome Profiler Mouse XL Cytokine Array
For research use only
Citations for Proteome Profiler Mouse XL Cytokine Array
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Customer Reviews for Proteome Profiler Mouse XL Cytokine Array (36)
4.9 out of 5
36 Customer Ratings
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Verified Customer | Posted 10/16/2025The Proteome Profiler Mouse XL Cytokine array performed with high sensitivity and reproducibility, enabling the simultaneous detection of multiple cytokines from limited sample volumes from our mouse retinal sampes.
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Verified Customer | Posted 09/17/2024High sensitivity with publishable results
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Verified Customer | Posted 05/23/2024mouse liver lysates
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Verified Customer | Posted 04/05/2024Performed on mammary tumor lysates.
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Verified Customer | Posted 06/29/2023I bought two boxes, and they're both great
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Verified Customer | Posted 06/21/2023easy to use kit
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Verified Customer | Posted 05/06/2023This product works very well
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Verified Customer | Posted 10/16/2022Conditioned medium of macrophages (36h) isolated from metastatic lungs.
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Verified Customer | Posted 08/28/2022This kit is really great!
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Verified Customer | Posted 08/07/2022Secretome of 4T1 murine breast cancer cells.
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Verified Customer | Posted 08/07/2022Cytokine array of the secretome of MLE12 cells.
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Verified Customer | Posted 07/11/2022
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Verified Customer | Posted 12/02/2021
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Verified Customer | Posted 10/21/2021
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Verified Customer | Posted 09/16/2021
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Verified Customer | Posted 08/10/2021We used the array kit for profiling inflammatory cytokines in plasma samples from mice. I trust that it works very well.
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Verified Customer | Posted 03/14/2021
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Verified Customer | Posted 09/17/2020Excellent antibody array to screen a multitude of cytokines, chemokines, and growth factors in one small strip of membrane. Readout is not a fluorescence so you don't need a special fluorescence reader. If you are doing Western blotting, you can do this easily.
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Verified Customer | Posted 02/12/2020Mouse breast tumor tissues
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Verified Customer | Posted 01/25/2020
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Verified Customer | Posted 01/06/2020Excellent array!!
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Verified Customer | Posted 06/06/2019
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Verified Customer | Posted 03/13/2019
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Verified Customer | Posted 12/05/2018
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Verified Customer | Posted 11/11/2018Excellent specificity and detection for large number of cytokines. Works perfectly with little optimization.
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Verified Customer | Posted 11/05/2018Pancreas lysate. 750ug protein/ membrane
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Verified Customer | Posted 07/18/2018
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Verified Customer | Posted 04/01/2018
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Verified Customer | Posted 03/22/2018
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Verified Customer | Posted 12/07/2017200 uL pooled serum from wild-type C57Bl/6 mice. 5 min exposure time
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Verified Customer | Posted 08/17/2017
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Verified Customer | Posted 07/17/2017
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Verified Customer | Posted 05/23/2017
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Verified Customer | Posted 11/28/2016150 uL of mouse serum
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Verified Customer | Posted 09/30/2016This is a great kit. It provides excellent reliability and a large number of cytokines, chemokines to screening in a single assay. I strongly recommend it.
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Verified Customer | Posted 08/26/2016The sample was cell culture supernatant collected from MEF.
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Protocols
View specific protocols for Proteome Profiler Mouse XL Cytokine Array (ARY028):
Refer to the product datasheet for complete product details.
Briefly, relative expression levels of mouse cytokines and chemokines in samples can be determined using the following procedure:
- Prepare membrane and incubate with prepared sample
- Incubate the membrane with Detection Antibody Cocktail
- Incubate the membrane array with Streptavidin-HRP
- Develop the membrane array with Chemi Reagents 1 and 2
- Expose the membrane array to autoradiography film
View Graphic Procedure
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
Other Supplies Required
Reagents
- Aprotinin
- Leupeptin (Catalog #EI002)
- Pepstatin (Catalog #EI003)
- Igepal® CA-630
- Deionized or distilled water
Materials
- Pipettes and pipette tips
- Gloves
- Plastic container with the capacity to hold 50 mL (for washing the arrays)
- Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides)
- Plastic wrap
- Absorbent lab wipes (KimWipes® or equivalent)
- Paper towels
- X-ray film (Kodak BioMax™ Light-1) or equivalent
- Flat-tipped tweezers
Equipment
- Rocking platform shaker
- Microcentrifuge
- Autoradiography cassette
- Film developer
- Flatbed scanner with transparency adapter capable of transmission mode
- Computer capable of running image analysis software and Microsoft Excel
Other Supplies Required for Cell Lysate Samples
- Phosphate-Buffered Saline (PBS)
- Lysis buffer (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 2 mM EDTA, 10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
Other Supplies Required for Tissue Lysate Samples
- PBS with protease inhibitors (10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
- Triton™ X-100
Procedure Overview
R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028)
- Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.
- Place each array membrane in a separate well of the 4-Well Multi-dish.
- Incubate for one hour on a rocking platform shaker.
- Add 0.5 mL Array Buffer 4 to each sample.
- Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6.
- Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples.
- Incubate overnight at 2 °C to 8 °C on a rocking platform.
- Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
- Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.
- Add 30 µL of Detection Antibody Cocktail to 1.5 mL of Array Buffer 4/6 for each array.
- Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish.
- Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail.
- Incubate for one hour on a rocking platform shaker.
- Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
- Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.
- Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish.
- Place the array membrane in the diluted Streptavidin-HRP solution.
- Incubate for 30 minutes on a rocking platform shaker.
- Wash each array membrane 3 times with 1X Wash Buffer in a separate container.
- Place the array membrane on a plastic sheet protector.
- Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane.
- Cover the membrane with the top sheet of the plastic protector.
- Incubate for 1 minute.
- Blot off excess Chemi Reagent Mix.
- Wrap the membrane and sheet protector in plastic wrap.
- Place the wrapped array membrane in an autoradiography film cassette and expose to X-ray film.
FAQs for Proteome Profiler Mouse XL Cytokine Array
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Q: Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
A: The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.